Erbin plays a critical role in human umbilical vein endothelial cell migration and tubular structure formation via the Smad1/5 pathway

Abstract

Angiogenesis is an important process in atherosclerosis. ErbB2 was proved to have an important role in vascular development, but it is still unclear whether Erbin expresses in vessels as well as its location and function in the vessels. In the current study，we investigated the location and function of Erbin in human umbilical veins. The human umbilical veins were prepared, and immunofluorescent analysis was performed to determine the expression of Erbin. Human umbilical vein endothelial cells (HUVECs) were cultured and the lentivirus (LV) containing Erbin RNAi was also prepared. After transfection with the lentivirus, CCK-8 assay and Annexin V-PI assay were used for cell proliferation and apoptosis, respectively. Cell migration was studied using the scratch wound healing assay and the transwell assay. The capillary-like tube formation assay was performed to illustrate the effect of Erbin on HUVEC tube formation.Expression of signaling pathway molecules was assessed with Western blot. The immunofluorescent analysis suggested that Erbin expressed in human umbilical veins and the majority of the Erbin is strongly colocalized in endothelial cells.Although knockdown of Erbin did not affect HUVEC proliferation and apoptosis, it significantly suppressed HUVEC migration and tubular structure formation. Erbin knockdown showed no effect on the ERK1/2 and Smad2/3 signaling pathways but significantly promoted Smad1/5 phosphorylation and J Cell Biochem. 2019;120:4654-4664. wileyonlinelibrary.com/journal/jcb 4654 |

Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation

“…Other than evidence for an interaction of erbin with integrin β4 in hemidesmosomes, it has few published links to adhesome components, and a potential role in IAC regulation has yet to be explored (Favre et al, 2001). Like scribble, erbin has been localized to the leading edge of cells and was identified in the meta-adhesome (one of seven datasets; Jin et al, 2019;Dan et al, 2010;Horton et al, 2015). However, considering that erbin is identified as a proximal interactor by a different subset of adhesome baits, and is not detected by BirA*-β-Pix and -GIT1, it is likely that the two proteins have different binding partners and perform different roles.…”

CircERBB2IP promotes post-infarction revascularization via the miR-145a-5p/Smad5 axis

“…ERBB2IP is a member of the leucine-rich repeat sequence and PDZ structural domain protein family and has a protective effect on cardiomyocyte apoptosis under the stimulation of catecholamines, 13 and it has been reported that ERBB2IP can promote angiogenesis. 14 Using the transcriptome dataset reported in a previous study, 11 we analyzed the circRNA expression profiles of neonatal and adult mouse hearts and screened the circRNA circERBB2IP, which is a circRNA containing 364 bases. It is produced by the head-to-tail splicing of exons 2, 3, and 4 of the parental gene ERBB2IP.…”

Sustained Release of VEGF to Promote Angiogenesis and Osteointegration of Three-Dimensional Printed Biomimetic Titanium Alloy Implants

“…To detect cellular activity and proliferation, live/dead cell staining (BestBio, Shanghai, China) and CCK-8 assay (Bioss, Beijing, China) were performed. As described in previous experiments ( Jin et al, 2019 ), the medium required for the experiment was endothelial cell medium (Sciencell Research Laboratories, China). Empty titanium (eTi), hydrogel-bound titanium (cTi), and hydrogel-bound titanium scaffolds loaded with VEGF (cTi/VEGF) were placed into 24-well plates.…”