Er-xian decoction drug-containing serum promotes Mc3t3-e1 cell proliferation and osteogenic differentiation via regulating BK channel

Ren, Ming-Shi; Xie, Huihui; Ding, Yulong; Li, Zi-Han; Liu, Bo

doi:10.1016/j.jep.2022.115887

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…Capsule also improves the bone growth factor bone morphogenetic protein 2 (BMP-2) expression in osteoporotic rats with fractures, enhances insulin-like growth factor-1 (IGF-1) expression, and promotes bone (45). EXD exerts estrogen-like effects and is effective in reducing bone loss by increasing BMD and improving bone microarchitecture as well as restoring serum levels of the osteoblast-secreted (OCN) protein (44); it also enhances the proliferation rate of osteoblast-like UMR-0 cells in rats (46), and promotes the proliferation of mouse embryonic osteoblast precursor cells, Mc3t3-e1 cells, and osteogenic differentiation by regulating the BK channel (47). EXD also activates the Insulin-like growth factor 1 receptor/Phosphatidylinositol 3-kinase/Protein kinase B(IGF1/PI3K/AKT) signaling pathway by increasing serum IGF1 concentration and tibial Insulin-like growth factor 1 receptor (IGF1R), PI3K, and AKT expression, thereby promoting osteogenic differentiation and proliferation of osteoblasts.…”

Section: Active Ingredients and Mechanisms Of Action Of Tcmmentioning

confidence: 99%

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Zhang,

Li,

Xie

et al. 2024

Front. Endocrinol.

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ObjectiveThe aims of this study were to evaluate the quality of osteoporosis guidelines on traditional Chinese medicine (TCM) drug therapies and to analyze the specific recommendations of these guidelines.MethodsWe systematically collected guidelines, evaluated the quality of the guidelines using the Appraisal of Guidelines Research and Evaluation (AGREE) II tool, and summarized the recommendations of TCM drug therapies using the Patient-Intervention-Comparator-Outcome (PICO) model as the analysis framework.Results and conclusionsA total of 20 guidelines were included. Overall quality evaluation results revealed that four guidelines were at level A, four at level B, and 12 at level C, whose quality needed to be improved in the domains of “stakeholder involvement”, “rigor of development”, “applicability” and “editorial independence”. Stratified analysis suggested that the post-2020 guidelines were significantly better than those published before 2020 in the domains of “scope and purpose”, “stakeholder involvement” and “editorial independence”. Guidelines with evidence systems were significantly better than those without evidence systems in terms of “stakeholder involvement”, “rigor of development”, “clarity of presentation” and “applicability”. The guidelines recommended TCM drug therapies for patients with osteopenia, osteoporosis and osteoporotic fracture. Recommended TCM drugs were mainly Chinese patent medicine alone or combined with Western medicine, with the outcome mainly focused on improving bone mineral density (BMD).

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Section: Active Ingredients and Mechanisms Of Action Of Tcmmentioning

confidence: 99%

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Zhang,

Li,

Xie

et al. 2024

Front. Endocrinol.

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“…They can differentiate into bone, cartilage, fat, nerve and muscle cells (Ning et al, 2022). MC3T3-E1 precursor osteoblasts are the main cells used in osteogenic differentiation studies performed in vitro (Ren et al, 2023). The development of the chronic inflammatory environment induced by wear particles can inhibit the differentiation of BMSCs into osteoblasts and promote the differentiation and proliferation of osteoclasts.…”

Section: The Dual Role Of Autophagy In Osteogenic Differentiationmentioning

confidence: 99%

Regulatory signaling pathways of osteoblast autophagy in periprosthetic osteolysis

Gu,

Wu,

Xie

et al. 2024

Front. Cell Death

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Periprosthetic osteolysis is a difficult-to-treat complication of arthroplasty. The pathological mechanisms of periprosthetic osteolysis are mainly weakened function of osteoblasts and excessive activation of osteoclasts. Many studies have demonstrated that the imbalance between the formation of bone by osteoblasts and the absorption of bone by osteoclasts is the direct cause of osteolytic diseases. Autophagy, as an important self-protective cellular mechanism, has significant effects on the regulation of osteoblast function, such as osteoblast differentiation, proliferation, and apoptosis. Osteoblasts, which play an important role in maintaining bone homeostasis, have attracted increasing attention in recent years. Up till now, Several signaling pathways have been proved to regulate autophagy of osteoblasts, including the AMPK, NF-κB, FoxO3 and other signaling pathways. This article reviews the recent progress in understanding osteoblast autophagy and mitophagy in the context of periprosthetic osteolysis and the signaling pathways which are involved in these processes. By summarizing previous studies describing the mechanism underlying osteoblast autophagy, we wish to contribute new therapeutic ideas and potential therapeutic targets for periprosthetic osteolysis.

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“…Er-xian Decoction (EXD) is a well-known prescription drug widely used to prevent and treat climacteric syndrome and osteoporosis in China. It is now suggested that its osteogenesis-promoting effects are attributable to upregulating BKα expression [ 160 ].…”

Section: Calcium-dependent Potassium Channelsmentioning

confidence: 99%

Ca2+-Activated K+ Channels in Progenitor Cells of Musculoskeletal Tissues: A Narrative Review

Takács

Kovács

Ebeid

et al. 2023

IJMS

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Musculoskeletal disorders represent one of the main causes of disability worldwide, and their prevalence is predicted to increase in the coming decades. Stem cell therapy may be a promising option for the treatment of some of the musculoskeletal diseases. Although significant progress has been made in musculoskeletal stem cell research, osteoarthritis, the most-common musculoskeletal disorder, still lacks curative treatment. To fine-tune stem-cell-based therapy, it is necessary to focus on the underlying biological mechanisms. Ion channels and the bioelectric signals they generate control the proliferation, differentiation, and migration of musculoskeletal progenitor cells. Calcium- and voltage-activated potassium (KCa) channels are key players in cell physiology in cells of the musculoskeletal system. This review article focused on the big conductance (BK) KCa channels. The regulatory function of BK channels requires interactions with diverse sets of proteins that have different functions in tissue-resident stem cells. In this narrative review article, we discuss the main ion channels of musculoskeletal stem cells, with a focus on calcium-dependent potassium channels, especially on the large conductance BK channel. We review their expression and function in progenitor cell proliferation, differentiation, and migration and highlight gaps in current knowledge on their involvement in musculoskeletal diseases.

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Er-xian decoction drug-containing serum promotes Mc3t3-e1 cell proliferation and osteogenic differentiation via regulating BK channel

Cited by 6 publications

References 43 publications

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Osteoporosis guidelines on TCM drug therapies: a systematic quality evaluation and content analysis

Regulatory signaling pathways of osteoblast autophagy in periprosthetic osteolysis

Ca2+-Activated K+ Channels in Progenitor Cells of Musculoskeletal Tissues: A Narrative Review

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