ER-to-Golgi transport visualized in living cells

Presley, John F.; Cole, Nelson B.; Schroer, Trina A.; Hirschberg, Koret; Zaal, Kristien J.M.; Lippincott‐Schwartz, Jennifer

doi:10.1038/38001

Cited by 972 publications

(1,008 citation statements)

References 1 publication

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“…These travel inwards to the central and juxtanuclear Golgi apparatus along microtubules with the help of the dynein/dynactin motor complex (Presley et al, 1997;Scales et al, 1997). The converse process, slow constitutive recycling from the Golgi apparatus back to the ER, does not seem to involve long-range processes, at least not any that can be easily detected.…”

Section: Rab6mentioning

confidence: 99%

Regulation of Microtubule-dependent Recycling at theTrans-Golgi Network by Rab6A and Rab6A'

Young

Stauber

Nery

et al. 2005

MBoC

102

122

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The small GTPase rab6A but not the isoform rab6A has previously been identified as a regulator of the COPIindependent recycling route that carries Golgi-resident proteins and certain toxins from the Golgi to the endoplasmic reticulum (ER). The isoform rab6A has been implicated in Golgi-to-endosomal recycling. Because rab6A but not A , binds rabkinesin6, this motor protein is proposed to mediate COPI-independent recycling. We show here that both rab6A and rab6A GTP-restricted mutants promote, with similar efficiency, a microtubule-dependent recycling of Golgi resident glycosylation enzymes upon overexpression. Moreover, we used small interfering RNA mediated down-regulation of rab6A and A' expression and found that reduced levels of rab6 perturbs organization of the Golgi apparatus and delays Golgi-to-ER recycling. Rab6-directed Golgi-to-ER recycling seems to require functional dynactin, as overexpression of p50/dynamitin, or a C-terminal fragment of Bicaudal-D, both known to interact with dynactin inhibit recycling. We further present evidence that rab6-mediated recycling seems to be initiated from the trans-Golgi network. Together, this suggests that a recycling pathway operates at the level of the trans-Golgi linking directly to the ER. This pathway would be the preferred route for both toxins and resident Golgi proteins. INTRODUCTIONAnterograde flow of material through the exocytic pathway is offset by the constant recycling of both proteins and lipids. This recycling helps to ensure that steady-state distributions of resident proteins are maintained within the pathway at the same time as keeping a lipid balance. Besides intra-Golgi recycling between cisternae (Hoe et al., 1995;Love et al., 1998;Lanoix et al., 1999;Lin et al., 1999), Golgi glycosylation enzymes also can recycle directly to the endoplasmic reticulum (ER). It is estimated that at least 5% of Golgi resident enzymes reside in the ER, at steady state (Pelletier et al., 2000). This pool constitutes recycled material originating from the Golgi apparatus (Cole et al., 1998;Storrie et al., 1998).The extent of ER recycling of Golgi resident membrane proteins is sufficient to allow for complete assembly of functional Golgi stacks. Thus, upon addition of nocodazole to depolymerize microtubules, the central and juxtanuclear Golgi apparatus relocates to the 100 or so peripheral ER exit sites scattered throughout the cell. This microtubule-independent relocation occurs in the absence of any detectable elements tracking outwards from the central Golgi apparatus. Rather, the Golgi apparatus undergoes a molecular deconstruction in the trans-to-cis-direction, i.e., resident glycosylation enzymes of the trans-cisternae/trans-Golgi network (TGN) relocate with faster kinetics than do enzymes of the medial-cisternae (Yang and Storrie, 1998). Enzymes seemingly enter the ER close to the microtubule organizing center (MTOC), diffuse through the ER, and then reemerge at peripheral ER exit sites. Here, Golgi stacks are then reassembled into discrete, yet fully functiona...

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Section: Rab6mentioning

confidence: 99%

Regulation of Microtubule-dependent Recycling at theTrans-Golgi Network by Rab6A and Rab6A'

Young

Stauber

Nery

et al. 2005

MBoC

102

122

View full text Add to dashboard Cite

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“…Two groups of mutants were observed, with a strong inhibition of FRET with Ala substitutions in positions 8, 10, 11, 12, 15, 19 and 20 (group 1), and less inhibition in positions 13,16,17,18, and 21 (group 2) ( Supplementary Fig. 4).…”

mentioning

confidence: 99%

“…As p24 acts as membrane machinery for the formation of COPI vesicles, we analysed if a loss of binding of SM 18 would affect transport of biosynthetic cargo 18,19 . We analysed transport of vesicular stomatitis G (VSV-G) protein 20,21 to determine the transport rates in HeLa cells stably transfected with full-length p24 wt or p24L17F, N-terminally fused to YFP.…”

mentioning

confidence: 99%

Molecular recognition of a single sphingolipid species by a protein’s transmembrane domain

Contreras

Ernst

Haberkant

et al. 2012

Nature

337

315

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This is an accepted version of a paper published in Nature. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination.Citation for the published paper: von Heijne, G., Contreras, X., Ernst, A., Haberkant, P., Björkholm, P. et al. (2012) "Molecular recognition of a single sphingolipid species by a protein's transmembrane domain" Nature, 481 (7382): 525-529 URL: http://dx

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“…The authors suggested that Nme2 participates in the formation of a proteinaceous scaffold along which ER exit sites are organized. Another possibility is that ER-to-Golgi transport requires microtubules (Presley et al 1997). Interestingly, one of the earliest observed properties of Nme, in this case awd and the bovine NDPK, was its association with microtubules (Biggs et al 1990;Nickerson and Wells 1984).…”

Section: Future Perspectives: a Potential Unifying Themementioning

confidence: 99%

Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: studies on development, cardiovascular disease, and cancer metastasis exemplified

Hsu

Steeg

Zollo

et al. 2015

Naunyn-Schmiedeberg's Arch Pharmacol

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ER-to-Golgi transport visualized in living cells

Cited by 972 publications

References 1 publication

Regulation of Microtubule-dependent Recycling at theTrans-Golgi Network by Rab6A and Rab6A'

Regulation of Microtubule-dependent Recycling at theTrans-Golgi Network by Rab6A and Rab6A'

Molecular recognition of a single sphingolipid species by a protein’s transmembrane domain

Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: studies on development, cardiovascular disease, and cancer metastasis exemplified

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