Equine frozen semen: Freezability and fertility field results

Vidament, Marianne; Dupere, A.M.; Julienne, P.; Evain, A.; Noue, P.; Palmer, Eric

doi:10.1016/s0093-691x(97)00319-1

Cited by 148 publications

(96 citation statements)

References 7 publications

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“…This includes morphological and functional changes of stallion spermatozoa, which was shown in a parallel study (Blottner et al, 2001) and is in accordance with many reports (Amann and Pickett, 1987;Samper et al, 1991;Harkema and Boyle, 1992;Wöckener et al, 1992;Braun et al, 1994;Dobrinski et al, 1995;Vidament et al, 1997). The results of the present study show a distinct decline especially in the number of motile spermatozoa after freezing and thawing, as is expected.…”

Section: Discussionsupporting

confidence: 93%

“…One of the prerequisites for successful preservation of stallion semen is a high initial quality, but this shows considerable differences between individual stallions. The distinct variation in freezability of stallion semen is one of the major factors determining the success of artificial insemination with frozen spermatozoa (Samper et al, 1991;Vidament et al, 1997). Furthermore, the normal equine breeding season begins in the spring and extends through mid-summer.…”

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confidence: 99%

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Characterisation of movement pattern and velocities of stallion spermatozoa depending on donor, season and cryopreservation

Warnke

Tuchscherer

Alm

et al. 2003

Acta Veterinaria Hungarica

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The aim of the study was to compare different types of movement pattern and velocities of stallion spermatozoa depending on cryopreservation during breeding and non-breeding season. Ejaculates were collected from four stallions during May (n = 24) and December (n = 24). Parameters of sperm movement were evaluated by computer-aided sperm analysis (CASA) system, and included percentages of motile spermatozoa, different patterns of motility, the velocity, linearity (LIN), amplitude of lateral head displacement (ALH) and beat-cross frequency (BCF). In winter the average percentages of motility were slightly higher compared to the breeding season in May (70.8 ± 12.7% vs. 66.8 ± 12.2%, respectively). Cryopreservation and thawing led to a significant decrease in the number of motile sperm to 11.3 ± 5.8% in May and 15.6 ± 7.0% in December. The pattern of motility was also changed. Detailed analysis by CASA demonstrated that cryopreservation resulted in a shift from the proportions of linear to more non-linear motile spermatozoa and to a significant increase of local motile and hyperactivated spermatozoa. Mean velocity of fresh motile spermatozoa differed between May and December (119.1 ± 43.9 vs. 164.4 ± 66.4 µm/sec, respectively; P < 0.05). Cryopreservation and thawing led to a slight increase of curvilinear velocity (VCL) and straight line velocity (VSL). The motility analysis has shown that the parameters BCF and ALH were highly correlated in stallion spermatozoa (r = -0.67; P < 0.001). The BCF of stallion spermatozoa was slightly reduced in the non-breeding season. Altogether, the influence of factors on the motility of stallion spermatozoa has the following rank order: cryopreservation (P < 0.0001) > stallion (P < 0.001) > season (P < 0.05).

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Characterisation of movement pattern and velocities of stallion spermatozoa depending on donor, season and cryopreservation

Warnke

Tuchscherer

Alm

et al. 2003

Acta Veterinaria Hungarica

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show abstract

“…6), artificial insemination (fresh, cooled and frozen semen) by hand or free running natural matings, had a highly significant effect on numerical productivity. On the contrary to the experimental results, [9][10][11], AI with fresh semen showed the best results in the field. AI with Fertility in French horse breeding 589 cooled and frozen semen did not differ from that in natural by hand mating.…”

Section: Resultscontrasting

confidence: 82%

Statistical analysis of some factors affecting the number of horse births in France

Langlois

Blouin

2004

Reprod. Nutr. Dev.

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-Declarations of matings (535 746) and 308 549 consecutive declarations of birth from 1989 to 1999 were analysed by logistic regression in order to determine the effects of year, breed and age of parents on numerical productivity (the number of foals declared per mated mare per year). For the years 1994 to 1999, the status of the mare, type of mating and month of first mating, were also available. The effect of inbreeding and, for warm-blooded horses, the effect of the level of performances or the effect of the level of breeding value estimation were also analysed. The main results are the following: numerical productivity progressed in France more for draught breeds than for saddle breeds and trotters. Thoroughbreds progressed less and just reached the level of significance. Cold-blooded horses, however, appeared less productive than warm-blooded horses for which thoroughbreds were at the lower level. It cannot be concluded if this figure reveals biological differences in fertility or if it is only the result of differences in managing the official declarations. For warm-blooded horses, the absence of negative relationships between the trends of selection and numerical productivity results appeared clearly. A high performance level for the mare was positively associated with higher productivity results in sport and trotting horses and showed no significant influence for galloping horses. The relationships with breeding value estimation illustrated the same trends.horse / fertility / logistic model / factors of variation / selection / inbreeding

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“…Although a wide variation between the stallions is generally recognized, practical experience has shown that the mares also affect the outcome. Older mares [1,2], and particularly old maiden mares (> 8 years of age), have a poor chance 244 K. Güvenc et al of conceiving with frozen semen [3]. The diagnosis most frequently encountered in uterine biopsies of older barren mares is widespread chronic degenerative endometrosis characterized by periglandular fibrosis and cystic distension of the glands [4].…”

Section: Introductionmentioning

confidence: 99%

Effect of frozen semen on the uterus of mares with pathological uterine changes

2004

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-Pregnancy rates after frozen semen inseminations (AI), particularly in older and problem mares, are lower than after fresh semen AI. Uterine contractility and the inflammatory reaction after frozen semen insemination were studied in two groups of mares: the abnormal group comprised of 6 old barren mares categorized in biopsy category IIB or III, and the control group including 6 reproductively normal young maiden mares in biopsy category I or IIA. All 12 mares were inseminated in the first cycle with 2 mL of phosphate-buffered saline (PBS) and in their second cycle with 2 mL of frozen semen containing 800 × 10 6 spermatozoa. Before and 1, 2, 4, 8, and 20 to 24 h after this treatment, all mares were examined by ultrasonography for intrauterine fluid accumulations (IUFA). The examinations were videotaped to count the number of uterine contractions later. Uterine fluid was obtained by tampon before treatment, and by the tampon method followed by uterine lavage after the last examination. Fluids were cultured bacteriologically, and polymorphonuclear leukocytes (PMN) were counted. Trypsin-inhibitor capacity (TIC), lysozyme concentration, and β-glucuronidase (BGase) and N-acetyl-β-D-glucosaminidase (NAGase) activities were determined in frozen-thawed tampon and lavage fluids. Both treatments induced significant neutrophilia in the uterine lumen. Although PMN concentrations were numerically higher after frozen semen AI than after PBStreatment, the difference was not significant. There was not any difference between the mare groups either. The amount of IUFA differed only in the normal group between frozen semen AI and PBS treatment, and between 0-and 24-h samples for frozen semen AI. Although abnormal mares showed consistently more fluid than normal mares, this difference was not significant. Uterine contractions and enzyme concentrations between groups did not differ. None of the variables showed significant differences between the normal and abnormal mares in their reaction to frozen semen AI.horse / insemination / frozen semen / uterine fluid / inflammation / leukocyte

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Equine frozen semen: Freezability and fertility field results

Cited by 148 publications

References 7 publications

Characterisation of movement pattern and velocities of stallion spermatozoa depending on donor, season and cryopreservation

Characterisation of movement pattern and velocities of stallion spermatozoa depending on donor, season and cryopreservation

Statistical analysis of some factors affecting the number of horse births in France

Effect of frozen semen on the uterus of mares with pathological uterine changes

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