Equine chondrocyte activation by a variety of stimuli

May, S. A.; Hooke, R.E.; Lees, P.

doi:10.1016/0007-1935(92)90026-w

Cited by 14 publications

(13 citation statements)

References 25 publications

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“…Equine IL‐1 has been identified and characterized, 8,9,49 and exhibits a similar molecular‐weight, isoelectric point, and >80% homology with human IL‐1 9,22 . Cross‐reactivity of the anti‐human IL‐1 antibody to equine‐origin IL‐1 would be expected 16,37–40 . Parallelism of ELISA‐derived concentrations of equine proteins with human proteins has been reported for thrombin–antithrombin III complexes 50,51 .…”

Section: Discussionmentioning

confidence: 93%

Synovial Fluid Cytokines and Eicosanoids as Markers of Joint Disease in Horses

2001

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The ability of a single value from a joint in an adult horse predicting the presence of joint disease was often good (.5-.75), and was excellent (> or =.75) for IL-6 and PGE(2). TNF-alpha and IL-1beta were no more effective than white blood cell count in screening for joint disease. IL-6 was the most sensitive and specific for joint disease and could be an excellent screening test for the presence of joint disease when lameness is difficult to identify or is intermittent. PGE(2) would be a functional screening test for the presence of any joint disease and offers a differentiating feature because values were not influenced by white blood cell count. PGF(1)-alpha values > 16.5 pg/mL identified chronic severe joint disease and may be clinically useful when there are minimal radiographic changes but substantial articular cartilage degradation.

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Section: Discussionmentioning

confidence: 93%

Synovial Fluid Cytokines and Eicosanoids as Markers of Joint Disease in Horses

2001

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“…NO and PGE2 were shown to be produced by synoviocytes and chondrocytes of different species as an immediate reaction to a variety of stimuli (Stadler et al, 1991 ;Evans, 1992;May eI al., 1992', Mort et al, 1993Palmer et al, 1993;Frean et al, 1997). Most of these studies were carried out with normal synovial membrane and afticular cartilage specimens that were subjected to stimulation with cytokines, such as interleukin-1 (lL-1) (Bandara et al, 1989;May et al, 1992;Stefanovic-Racrc et al, 1993b;Frean eI al., 1997), tumor necrosis factor B [NF-B) $tadler et al, 1991) and lipopolysaccharides from bacterial wall fragments (LPS) (Maier et al, 1992', May et al, 1992', BirkedalHansen, 1993MacDonald et al, 1994;Frean et al, 1997). Measurements of NO production by monolayer cell cultures of equine synovial membrane and articular cartilage after stimulation with lL-1 B and LPS showed that both cell types were capable of releasing this mediator, but that synoviocytes produced less than chondrocytes (Evans et al, 1996;Frean et al, 1997).…”

Section: Pferdeheilkunde 15mentioning

confidence: 99%

Temporal production of nitric oxide and Prostaglandin E2 in media of explant cultures of equine synovial membrane and articular cartilage

Rechenberg¹,

McIllwraith²,

Akens³

et al. 1999

PHK

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SummaryThe local mediators nitric oxide (NO) and prostaglandin E, (PGE ) play a role in the cascade of extracellular matrix breakdown in articular cartilage. In the present study the spontaneous production of NO and PGE, in explant cultures of equine synovial membrane and articular cartilage and their concentration over tjme n culture was investigated and correlated. Synovial membrane and articular cartilage was harvested from metacarpophalangeal jonts of 33 horses durlng arthroscopic surgery (n=4) or after being slaughtered/euthanised for reasons other than infectious or metabolic dlsease (n=29). Trssues were cultured as explant cuJtures in the presence of 4 ml Ham's F 12 medium, supplemented with streptomycin (100 pg/ml) and penicillin (100 U/ml) and incubated in a 5% CO2 and water saturated atmosphere for 8 days. Re sults showed that generally equine synovial membrane produced less NO than articular cartilage explant cultures with comparable wet weights/ml of media, but significantly more PGE, (P<0.05). The concentrations of both mediators were relatively stable over the first 4 days of culture, at least in the group of 25 mg wet wdghvml of media. In this group, there was a weak tendency noticed for NO to decrease, and PGE, to increase over time with day 4 being the intersection for both. Tissue cultures of articular cartilage with 12.5 mg wet weight/ml of culture media produced less reliable results, especially with NO measurements, which was thought to be associated with the sensitivity of the assay. lt is recommended to collect conditioned media for the measurements of NO and PGE, within the first 4 days of explant culture, since atter this time period culture effects may mask the results at least for NO measurements. Both mediators may be factors that play an impor tant role in the local med ation of ioint inflammations. Keywords:nitric oxide, prostaglandin Er, synovial membrane, articular cartilage, equine Die zeitlich bedingte Produktion von Stickoxid (NO) und Prostaglandin E, (PGEr) in Medium von Gewebekultur€n aus Synovialmembran und GelenkknorDel von PferdenDie lokalen Mediatoren, Stickoxid (nitric oxide = NO) und Prostaglandin E, (PGEr) spielen eine Rolle in der Kaskade des Abbaus der extrazellulären lvlatrix von Knorpel und Knochen. In der vorliegenden Studie wurde die spontane Produktion und Konzentration von NO und PGE2 in Gewebekulturen aus Synovialmembran und Gelenkknorpel von Pferden über eine Zeit von B Tagen untersucht und korreliert. Bei 33 Pferden wurde während der Arthroskopie des Gelenkes {n=4) oder zum Zeitpunkt der Schlachtung (n=29) Synovialmembran und Gelenkknorpel aus dem Fesselgelenk entnommen. Das Gewebe wurde in der Gewebekultur ('12.5 mg/ml Medium und 25 mg/ml Medium) n Ham's F-1 2 Medi um, angereichert mit Streptomycin (100 pg/ml) und Penicillin (100 U/ml), unter Standardbedingungen von 5% CO, und mit Wasser gesättig-ter Atmosphäre für B Tage inkubiert. Es wurde gezeigt, dass allgemein die Synovialmembran Gewebekulturen deutlich weniger NO produzierten als die Gelenkknorpel Gew...

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“…1 In the Thoroughbred racehorse industry worldwide, musculoskeletal injury accounts for the highest proportion of days lost from training [2][3][4][5] and is the most common cause of athletic morbidity. [9][10][11][12][13] Intra-articular analgesics (mepivacaine, lidocaine, and bupivacaine 14 ) and intraarticular therapeutic agents (corticosteroids, polysulfated Anti-inflammatory and analgesic effects of intra-articular injection of triamcinolone acetonide, mepivacaine hydrochloride, or both on lipopolysaccharide-induced lameness in horses glycosaminoglycans, and hyaluronic acid derivatives) remain important aids in the diagnosis of acute synovitis and treatment of affected horses and help in the prevention of additional degenerative articular changes. [9][10][11][12][13] Intra-articular analgesics (mepivacaine, lidocaine, and bupivacaine 14 ) and intraarticular therapeutic agents (corticosteroids, polysulfated Anti-inflammatory and analgesic effects of intra-articular injection of triamcinolone acetonide, mepivacaine hydrochloride, or both on lipopolysaccharide-induced lameness in horses glycosaminoglycans, and hyaluronic acid derivatives) remain important aids in the diagnosis of acute synovitis and treatment of affected horses and help in the prevention of additional degenerative articular changes.…”

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confidence: 99%