Equilibrium Dialysis: A New Approach

Coombs, Timothy J.; Coulson, Christopher J.

doi:10.1042/bst0061048

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1980

1981

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Cited by 3 publications

(3 citation statements)

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“…In addition, these units can be used to measure the binding of radioactive drug to whole plasma (6) and, potentially, the binding of any ligand to a macromolecule. The alternative is to purchase an expensive instrument.…”

Section: Resultsmentioning

confidence: 99%

Correlation of hydrophobicity with protein binding for clorobiocin analogs

Coulson

Smith

1980

Journal of Pharmaceutical Sciences

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Section: Resultsmentioning

confidence: 99%

Correlation of hydrophobicity with protein binding for clorobiocin analogs

Coulson

Smith

1980

Journal of Pharmaceutical Sciences

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“…The perspex equilibrium dialysis units, previously described in detail (Coombs & Coulson, 1978) are made in two halves, which are screwed together with a sheet of Spectrapor 2 membrane (MSE/Fisons, Crawley, Sussex, UK) between them. Solutions of macromolecule and small molecules are placed either side of the membrane and left to equilibrate at a convenient temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The perspex equilibrium dialysis units, previously described in detail (Coombs & Coulson, 1978) Blood was obtained from human volunteers between 10.00 and 13.00 h and immediately centrifuged to give plasma without coagulation. Anticoagulants were not used in case they altered the binding characteristics of the drug.…”

Section: Methodsmentioning

confidence: 99%

Blood plasma binding of acebutolol and diacetolol in man.

Coombs¹,

Coulson²,

Smith³

1980

Brit J Clinical Pharma

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1 Acebutolol or diacetolol were added to fresh human plasma in varying concentrations and their extent of binding at 25 degrees C measureed by an equilibrium dialysis technique. 2 The extent of binding for both compounds was shown to be very low, being 11‐19% for acebutolol and 6‐ 9% for diacetolol. 3 Partition coefficients were measured in an n‐ octanol/phosphate buffer (0.05M, pH 7.4) solvent system. For a cebutolol, P = 0.62 and for diacetol, P = 0.08. 4 The very low plasma binding is in accord with the hydrophilic partition coefficients of these compounds.

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Coumarin antibiotic binding to human albumin

Coulson¹,

Smith²

1981

Biochemical Pharmacology

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Equilibrium Dialysis: A New Approach

Cited by 3 publications

References 0 publications

Correlation of hydrophobicity with protein binding for clorobiocin analogs

Correlation of hydrophobicity with protein binding for clorobiocin analogs

Blood plasma binding of acebutolol and diacetolol in man.

Coumarin antibiotic binding to human albumin

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