Epstein‐Barr virus‐associated complement‐fixing and nuclear antigens in Burkitt lymphoma biopsies

Abstract

Cells from Burkitt lymphoma ( B L ) biopsies were examined for Epstein-Barr virus ( E B V)-associated antigens by complement fixation (CF) tests

Continuous Lymphoid Cell Lines with Characteristics of B Cells (Bone-Marrow-Derived), Lacking the Epstein-Barr Virus Genome and Derived from Three Human Lymphomas

“…It has been derived from the biopsy of an African patient with the diagnosis of Burkitt's lymphoma, JA (KCC 1490) (8,20). In previous nucleic acid hybridization studies and EBNA tests, repeated biopsies of this patient were EBV DNA and EBNA negative.…”

“…The history and the morphological and growth properties of these lines differed from the usual, EBV-genome-carrying human lymphoblastoid cell lines and it was therefore of interest to study whether these lines did carry the viral genome. (8,20). It was therefore of great interest to determine whether this cell line was representative for the virus-genome-negative biopsy or was merely the outgrowth of an admixed, nonrepresentative EBV-genome-carrying lymphoid cell-type.…”

Direct Evidence for the Presence of Epstein-Barr Virus DNA and Nuclear Antigen in Malignant Epithelial Cells from Patients with Poorly Differentiated Carcinoma of the Nasopharynx

“…Tests for the EBV-determined nuclear antigen (EBNA) were carried out on smear preparations, fixed in methanol-acetone, as described (4,5). …”

“…DNA of Epstein-Barr virus (EBV) has been regularly detected by nucleic acid hybridization in African Burkitt lymIhoma cells (1)(2)(3), which also contain a characteristic EBVdetermined nuclear antigen (EBNA) (3)(4)(5). Since Burkitt lymphoma is known to have a single cell origin (6), this disease can be regarded as a malignant proliferation of a clone carrying the EBV genome.…”

Surface marker characteristics and Epstein-Barr virus studies of two established North American Burkitt's lymphoma cell lines.

“…The presence of EBNA in the SU-AmB-2 cells was not recognized initially because the fluorescent staining of more than 90% of the nuclei was weak and punctate, rather than brilliant and diffuse as in all other EBNA-positive lines and biopsies examined to date (24,30,31). However, the fact that this reaction, though atypical, was consistently found with eight VCA-positive AmBL sera and not with three VCA-negative AmBL sera established that it was due to EBNA.…”