Epstein–Barr virus-associated adenocarcinomas and squamous-cell lung carcinomas

Abstract

The association of Epstein-Barr virus with pulmonary neoplasms has been restricted to lymphoepithelioma-like carcinomas in Asian patients. We have selected 19 pulmonary adenocarcinomas and squamous-cell carcinomas from 1545 pulmonary neoplasms diagnosed from 1996 to 2007 in an occidental population. All of them showed a low-power appearance confusing between an epithelial and a lymphoid neoplasm, with a dense lymphocytic infiltrate intermingled with neoplastic cells giving an image akin to lymphoepithelial complexes. Five carcinomas presented typical features of Lymphoepithelioma-like lung carcinomas; but six cases could be classified as squamous-cell carcinomas and eight as adenocarcinomas. A semiquantitative polymerase chain reaction method, Early RNA genes 1 and 2 in situ hybridization as well as Latent membrane protein immunostaining for Epstein-Barr virus DNA, RNA and protein detection methods were used in every case. None of Lymphoepithelioma-like carcinomas showed positivity for Epstein-Barr virus in any used method. Otherwise four squamous-cell carcinomas and eight adenocarcinomas (12 cases) demonstrated viral sequences in polymerase chain reaction and/or in situ hybridization analysis in neoplastic cells. Moreover two adenocarcinomas also displayed human herpesvirus 6 DNA sequences coamplification in molecular analysis. Protein immunostaining was focally positive in only three cases. We performed the same analysis in 70 more cases of conventional pulmonary squamous-cell carcinomas and adenocarcinomas that gave negative results. In conclusion, a subset of pulmonary squamous-cell carcinomas and adenocarcinomas show Epstein-Barr DNA and/or RNA sequences in neoplastic cells. This finding expands the spectra of epithelial cell common tumours Epstein-Barr virus associated.

Prevalence of human papillomavirus, Epstein‐Barr virus, and cytomegalovirus in fine needle aspirates from lung carcinoma: A case–control study with review of literature

“…38 However, studies from the west have reported either a complete lack or a very low prevalence of EBV in lung carcinoma. 12,[39][40][41][42][43][44] The reported seroprevalence of CMV ranges from 50 to 90% in different countries of the world. 12 In the present study, 2 cases (2.7%) of lung carcinoma showed positivity for EBV DNA.…”

“…Review of Literature for EBV in Lung Carcinoma12,[39][40][41][42][43][44] ADC 5 adenocarcinoma, IHC 5 immunohistochemistry, ISH 5 in-situ hybridization, NISH 5 non in-situ hybridization, NEC 5 neuroendocrine carcinoma, RFLP 5 restriction fragment length polymorphism, PCR 5 polymerase chain reaction, q PCR 5 quantitative polymerase chain reaction, SB 5 southern blot, SCC 5 squamous cell carcinoma, mi RNA 5 micro RNA, SCLC 5 small cell lung carcinoma, LCLC 5 large cell lung carcinoma.Diagnostic Cytopathology DOI 10.1002/dc VIRUSES IN FINE NEEDLE ASPIRATES FROM LUNG CARCINOMA Diagnostic Cytopathology, Vol. 44, No 12…”

Pulmonary adenocarcinoma with massive lymphocytic infiltration: a case report with review of the literature of a rare histological entity with a peculiar biological behaviour

“…Gomez-Roman et al reported eight cases of lung adenocarcinoma with a diffuse lymphoid stroma and a tubuloglandular pattern, associated with EBV infection [14]. …”

“…In nine cases [11,12], EBV infection was ruled out by immunohistochemical or molecular biology techniques, and in the other eight cases reported in literature [14] EBV positivity was demonstrated in neoplastic tissue.…”

Considerations on the Link Between Merkel Cell Polyomavirus And Lung Cancer

“…A higher incidence of HPV in lung cancers has been reported in Asian cohorts [4]. Another human tumor virus, Epstein-Barr virus, is found in lung cancers, especially in lymphoepithelioma-like carcinomas, predominantly in Asian patients [5]. Recently, the presence of murine mammary tumor virus-like sequence was identified in 18% of the lung cancer from Mexican patients [6].…”