Prevalence of human papillomavirus, Epstein‐Barr virus, and cytomegalovirus in fine needle aspirates from lung carcinoma: A case–control study with review of literature

Gupta, Parikshaa; Haldar, Dipanjan; Naru, Jasmine; Dey, Pranab; Aggarwal, Ashutosh N.; Minz, Ranjana Walker; Aggarwal, Ritu

doi:10.1002/dc.23613

Cited by 11 publications

(12 citation statements)

References 40 publications

(106 reference statements)

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“…Of interest, a number of authors have reported findings similar to ours or even higher HPV positivity rates in lung cancer tissues: 21% in Italy, 30% in Finland, 49% in Norway, 69% and 19% in Greece . In addition, some recent publications have indicated a positive link between HPV and etiopathogenesis of lung carcinoma, supporting the present findings . However, others have failed to identify such a causative link .…”

Section: Discussionsupporting

confidence: 87%

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Shikova

Ivanova

Alexandrova

et al. 2017

Microbiology and Immunology

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A possible association between high-risk human papillomaviruses (HPV) and lung cancer has been investigated for decades with discrepant results. The aim of this study was to determine the prevalence of HPV16 and 18 in Bulgarian patients with lung cancer. Two hundred and nine biopsy specimens from patients with histologically proven lung cancer and without cancer were analyzed. Each sample was subjected to three parallel PCRs using broad spectrum GP5+/6+ primers and type-specific (TS) primers for HPV types 16 and 18. Of the 132 lung carcinoma samples, 33 (25%) were positive for HPV16 and/or HPV18 by TS PCR whereas only five (3.8%) samples were HPV positive by consensus PCR. All non-malignant controls were HPV negative. HPV18 was the more prevalent, being found in 11.4% of samples, followed by HPV16 in 9.1% samples; 4.5% of lesions were positive for both HPV16 and HPV18. HPV16/18 were most prevalent in small cell carcinoma (29.2%) and least prevalent in squamous cell carcinoma (23.3%). HPV was only detected in squamous cell carcinoma and adenosquamous carcinoma by consensus PCR. This study revealed a high HPV16/18 prevalence in lung carcinoma samples from Bulgarian patients when TS PCR was used to detect them. The difference between HPV positivity as detected by consensus and by TS PCR was significant, indicating the importance of methodological issues in explaining the discrepancies between previous studies. HPV18 was more common than HPV16. No association between HPV16/18 status and histopathological diagnosis was identified.

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Section: Discussionsupporting

confidence: 87%

Human papillomavirus prevalence in lung carcinomas in Bulgaria

Shikova

Ivanova

Alexandrova

et al. 2017

Microbiology and Immunology

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“…HPV infection is a strongly associated and numerous studies have shown that infection with HR-HPV genotypes represents an associated factor for its development ( Gillison et al, 2000 ; Vokes, Agrawal & Seiwert, 2015 ). In addition, other viruses with oncogenic potential such as EBV and MCPV that have been detected in the nasopharyngeal tract, tonsils, salivary glands, and lungs may play an important role ( Baez et al, 2015 ; Pezzuto et al, 2015 ; Gupta et al, 2016 ). At present, there are insufficient reports describing the epidemiology of viral coinfections in tumors of the larynx, so studies in this regard could contribute to clarify their association with the carcinogenic progression and to facilitate preventive strategies ( Bansal, Singh & Rai, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Infection and coinfection by human papillomavirus, Epstein–Barr virus and Merkel cell polyomavirus in patients with squamous cell carcinoma of the larynx: a retrospective study

Vazquez-Guillen

Palacios-Saucedo

Rivera-Morales

et al. 2018

PeerJ

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BackgroundHuman papillomavirus (HPV) is recognized as an important risk factor for laryngeal carcinogenesis. Although HPV-16 and 18 have been strongly implicated, the presence of other high-risk HPV (HR-HPV) genotypes or the coinfection with Epstein-Barr virus (EBV) or Merkel cell polyomavirus (MCPV) may increase the risk, but their etiological association has not been definitively established.MethodsWe characterized the genotype-specific HPV and the frequency of EBV and MCPV infections through the detection of their DNA in 195 laryngeal specimens of squamous cell carcinoma (SCC) histologically confirmed.ResultsHPV DNA was detected in 93 (47.7%) specimens. HPV-11 was the most frequent with 68 cases (73.1%), and HPV-52 was the most frequently HR-HPV found with 51 cases, which corresponds to 54.8% of all HPV-positive specimens. EBV DNA was detected in 54 (27.7%) tumor tissue specimens of which 25 (46.3%) were in coinfection with HPV. MCPV DNA was detected only in 11 (5.6%) cases of which 5 (45.4%) were in coinfection with an HR-HPV. No association between the presence of DNA of the three examined viruses and the patient smoking habits, alcohol consumption, age, the keratinization status, differentiation grade, or localization of the tumor in the larynx were found.DiscussionHPV-52 was the most prevalent HR-HPV, which may suggest that this and other genotypes in addition to HPV-16 and 18 could be considered for prophylaxis. However, further studies including non-cancer larynx cases and the evaluation of other molecular markers and viral co-infection mechanisms are needed to determine the role of the different HR-HPV genotypes, EBV, and MCPV in the etiology of SCC of the larynx.

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“…The low rate of HPV detection in our patient cohort was expected because HPV-positive HNSCC does not tend to form distant metastases and primary lung SCC also tends to be HPV-negative. 9,10,38 By contrast, the observation that correctly identifying the lung tumor origin depended on mutational profiling of TP53 exons other than 5 to 8 in 6 of 32 cases (19%) puts the common limitation of TP53 mutation analysis to exons 5 to 8 in question and shows that detection of TP53 mutations should comprise all coding exons in order to maximize the sensitivity of the assay. 35 Remarkably, a comparison of lung tumor origin diagnoses made with or without molecular genetic data revealed that 16 of 29 patients (55%) for whom a clear diagnosis could be made by HPV typing and mutational profiling had received an incorrect diagnosis of the lung tumor origin when this diagnosis was based on clinical and morphological data alone.…”

Section: Daher Et Almentioning

confidence: 99%