EPR Study of the Intensity of the Nitric Oxide Production in Rat Brain After Ischemic Stroke

Гайнутдинов, Х. Л.; Гаврилова, С.А.; Iyudin, V. S.; Golubeva, Anna V.; Davydova, M. P.; Jafarova, G. G.; Андрианов, В. В.; Koshelev, V. B.

doi:10.1007/s00723-011-0207-7

Cited by 24 publications

(12 citation statements)

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“…As, a result of their interaction within the body, DETC 2 -Fe 2+ water-insoluble complex is formed, capable of capturing NO with the formation of (DETC) 2 -Fe 2+ -NO stable radical, which is detected by EPR [Bruker ER 200E SRC in X-band (9.50 GHz) spectrometer at 77 oC]. The method was described by us earlier (Gainutdinov et al, 2011 , 2013 ). The trapping of NO was introduced 30 min before decapitation.…”

Section: Methodsmentioning

confidence: 99%

Molecular Bases of Brain Preconditioning

et al. 2017

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Preconditioning of the brain induces tolerance to the damaging effects of ischemia and prevents cell death in ischemic penumbra. The development of this phenomenon is mediated by mitochondrial adenosine triphosphate-sensitive potassium (KATP+) channels and nitric oxide signaling (NO). The aim of this study was to investigate the dynamics of molecular changes in mitochondria after ischemic preconditioning (IP) and the effect of pharmacological preconditioning (PhP) with the KATP+-channels opener diazoxide on NO levels after ischemic stroke in rats. Immunofluorescence-histochemistry and laser-confocal microscopy were applied to evaluate the cortical expression of electron transport chain enzymes, mitochondrial KATP+-channels, neuronal and inducible NO-synthases, as well as the dynamics of nitrosylation and nitration of proteins in rats during the early and delayed phases of IP. NO cerebral content was studied with electron paramagnetic resonance (EPR) spectroscopy using spin trapping. We found that 24 h after IP in rats, there is a two-fold decrease in expression of mitochondrial KATP+-channels (p = 0.012) in nervous tissue, a comparable increase in expression of cytochrome c oxidase (p = 0.008), and a decrease in intensity of protein S-nitrosylation and nitration (p = 0.0004 and p = 0.001, respectively). PhP led to a 56% reduction of free NO concentration 72 h after ischemic stroke simulation (p = 0.002). We attribute this result to the restructuring of tissue energy metabolism, namely the provision of increased catalytic sites to mitochondria and the increased elimination of NO, which prevents a decrease in cell sensitivity to oxygen during subsequent periods of severe ischemia.

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Section: Methodsmentioning

confidence: 99%

Molecular Bases of Brain Preconditioning

et al. 2017

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“…For the quantitative determination of NO and Cu in the brain, we used the method of electron paramagnetic resonance -EPR [6,22]. This method is based on the reaction of a radical (in this case, NO) with a spin trap, which is the Fe 2+ complex with diethyldithiocarbamate (DETC), which captures NO to form a stable ternary complex (DETC) 2 -Fe 2+ -NO in various animal tissues.…”

Section: Formation Of a No Complex With A Spin Trapmentioning

confidence: 99%

“…The sample weight was 100 mg. The amplitude of the EPR spectra was always normalized in proportion to the mass of the sample and the amplitude of the EPR signal of the reference sample [6].…”

Section: Measurements Of No and Cumentioning

confidence: 99%

“…These hypotheses concern problems of metabolism and imbalance of neurotransmitters, active transport of ions across membranes and an increase in the level of ionized calcium in neurons. The mechanisms of neuronal damage are diverse and are caused, including paradoxically, by the neurotoxicity of the signaling molecule glutamate, in the development of which nitric monoxide (NO) plays a key role [5][6][7]. NO is a highly chemically reactive free radical, which can act as an oxidizing agent as well as a reducing agent [8].…”

Section: Introductionmentioning

confidence: 99%

“…NO is a highly chemically reactive free radical, which can act as an oxidizing agent as well as a reducing agent [8]. The regulatory role of NO has been shown in the nervous, cardiovascular, and other systems [6,[9][10][11]. The main components of the NO cycle are NO-synthase and nitrite reductase enzymatic systems [7,9,10,12,13].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Perineural Stem Cell Implantation on Motor Activity and Content of NO and Copper in the Olfactory System After Brain Ischemia

Андрианов

Yafarova

Tokalchik

et al. 2021

Advances in Cognitive Research, Artificial Intelligence and Neuroinformatics

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It was shown by electron paramagnetic resonance (EPR) spectroscopy that one day after bilateral occlusion of the common carotid arteries (to simulate ischemia) in Wistar rats (group 1, n = 15) under ketamine-xylazine-acepromazine anesthesia (55.6, 5.5, 1.1 mg/kg, respectively, intraperitoneally), the content of nitric monoxide (NO) in the olfactory bulbs decreased, and recovered after two days. A similar trend was observed in Wistar rats (group 2, n = 15), which, simultaneously with ischemia modeling, were implanted intranasally with mesenchymal stem cells (MSCs). An intact group of rats (group 3, n = 15) was kept in the usual conditions of the vivarium and did not undergo surgical interventions. The content of copper in the olfactory bulbs of rats (for assessing the activity of superoxide dismutases 1 and 3) after ischemia modeling (group 1) tended to increase and maintain the increase for two days of observation. Intranasal administration of MSCs during the ischemia modeling was accompanied by a more significant increase in copper content on the first day after brain ischemia modeling, but after two days, a tendency towards restoration of the initial copper level was noted.Intranasal administrating of MSC simultaneously with ischemia modeling (group 2) was accompanied by a more rapid recovery of orientational-motor activity in experimental animals compared to rats in which only modeling of ischemic stroke was performed (group 1).

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