Changes of Nitric Oxide Content in the Rat Hippocampus, Heart and Liver in Acute Phase of Ischemia

Андрианов, В. В.; Pashkevich, S. G.; Yafarova, G. G.; Denisov, A. B.; Iyudin, V. S.; Bogodvid, Tatiana; Dosina, Margarita; Kulchitsky, Vladimir A.; Гайнутдинов, Х. Л.

doi:10.1007/s00723-016-0815-3

Cited by 12 publications

(13 citation statements)

References 52 publications

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“…1E). Therefore, additional formation of NO from L-arginine (that we previously proved with atomic force microscopy [8] ), did not contributed to activation of SC migration processes in hippocampus that could probably happen due to double role of NO in brain: adjusting character on the one hand and -on the other hand -peroxynitrite formation that activates protective mechanisms against hypoxia in the organism including initiation of SC migration processes [9,10] .…”

Section: Resultsmentioning

confidence: 70%

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Stukach¹,

Gainutdinov²,

Dosina³

et al. 2017

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Section: Resultsmentioning

confidence: 70%

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Stukach¹,

Gainutdinov²,

Dosina³

et al. 2017

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“…Tissue samples were immediately frozen in liquid nitrogen and transported under frozen conditions from Minsk to Kazan. In this state, the complex spin trap with NO ((DETC) -Fe -NO) was well preserved and the signal from the complex did not change for a month (Vanin et al 2002, Andrianov et al 2016, Mikoyan et al 1997. The measurements of the spectra of the complex (DETC) -Fe -NO were performed on the spectrometer ER 200 SRC Bruker in the X band (9.50 GHz) with the modulation of the magnetic field of 100kHz, the modulation of the amplitude of 2Hz, the microwave radiation power of 30mW, time constant of 200ms and the temperature of 77ºK in the Finger Dewar Bruker.…”

Section: Formation Of the Complex Of No With The Spin Trap In Rat Tismentioning

confidence: 99%

“…In the vital functions of animals, the role of NO is especially significant in the cardiovascular function (Reutov et al 2007, Andrianov et al 2008) and in nervous systems (Steinert et al 2010, Terpolilli et al 2012, Gainutdinov et al 2011. At the present time, the development of cerebral ischaemia and the following stroke is associated with impaired cerebral blood flow, as well as violations of its regulation by the NO system (Remizova et al 2011, Terpolilli et al 2012.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, there is no doubt that the role of the NO system in the pathogenesis of a number of diseases associated with vascular disorders is defining , Terpolilli et al 2012, Godínez-Rubí et al 2013. Previously, by the methods of EPR spectroscopy, our team has evaluated the effect of ischaemic stroke on the intensity of NO production in the tissues of the brain, heart and liver of rats in vivo (Andrianov et al 2016). It was shown that within 5 hours after modelling of ischaemia, a double reduction of NO production was observed in the tissues of the hippocampus, heart and liver and it was maintained for 3 days.…”

Section: Introductionmentioning

confidence: 99%

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Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult

Гайнутдинов¹,

Pashkevich²,

Андрианов³

et al. 2017

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Electron paramagnetic resonance (EPR) was used as a method for recording the content of the nitric oxide (NO) in hippocampal tissues of intact rats and rats after modelling of ischaemic and haemorrhagic stroke. Based on direct measurements of NO by EPR spectroscopy, it was shown that, within 5 hours after the onset of symptoms of ischaemic and haemorrhagic stroke, the formation of NO in the hippocampus was reduced by a factor of 2-3 and this reduction was maintained for a period of between 24 and 72 hours. The results show that a systemic character of a decrease in the intensity of NO production during the modelling of ischaemic events in the brain reflects the effects of central dysregulation of the functions at the level of the whole organism such that it is appropriate to consider implementing the correction of the vital systems of the body in a stroke. It has indicated that non-selective NO-synthase blocker L-NAME reduced the low level of NO production by a factor of 3 by its administration within 72 hours after post-ischaemic and haemorrhagic stroke. It was discovered however that L-NAME returns the level of NO production to baseline (control) by its administration within 5 hours after ischaemia.

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“…Дисмутирование супероксида с помощью цитозольного фермента Cu, Zn-COD (супероксиддисмутаза -СОД) является первичной и основной защитой от процессов свободнорадикального окисления, однако образующийся при избыточной продукции NO пероксинитрит способен инактивировать фермент COD и ускорять процессы свободнорадикального окисления [17]. Нами в данном исследовании показано, что содержание меди (как показателя уровня 1-й и 3-й субъединиц COD) в обонятельной луковице увеличивается в 1-е сутки после моделирования ишемиче- [38,43,49,64]. Нам представляется, что обнаружен тот временной отрезок развития процессов, связанных с ишемией мозга, когда целесообразно проводить эффективную терапию с привлечением доноров NO.…”

Section: материалы и методы исследованияunclassified

Content of nitric oxide and copper in the olfactory bulbs of the rats brain after modeling of cerebral stroke and intranasal administration of mesenchymal stem cells

Андрианов

Кульчицкий

Яфарова

et al. 2021

Regional blood circulation and microcirculation

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Introduction. With a decrease in the oxygen content in the inhaled air, violations of the cerebral blood flow, brain ischemia occurs, which can end in an ischemic stroke. Aim. Comparative analysis of the intensity of nitric oxide (NO) production and the copper content in the olfactory bulb tissues of the brain of male Wistar rats after modeling an ischemic stroke. Materials and methods. Modeling of ischemic stroke by ligation at the bifurcation level of both common carotid arteries and measuring the content of NO and copper by EPR spectroscopy. Results. The relative changes in the number of NO-containing complexes and the copper content were estimated from the integrated signal intensity of the complexes (DETC)2-Fe2+-NO and (DETC)2- Cu. A significant decrease by 47 % after 1 and 57 % after 2 days, respectively, in the NO content in the olfactory bulb of the rat brain was found after the ischemia modeling. The level of NO production in rats that underwent ischemia simulation with simultaneous intranasal administration of mesenchymal stem cells (MSCs) was also reduced by 51 % after 1 and 70 % after 2 days, respectively, after ischemia modeling. There was no significant difference in the NO content in the rats after ischemia modeling with simultaneous intranasal administration of MSCs compared to the ischemic rats. The copper content, which corresponds to the level of superoxide dismutase 1 and 3, in the rat’s olfactory bulb tended to increase after ischemia modeling and it persisted for two days of observation (an increase of 50 % in both cases). Intranasal administration of MSCs was accompanied by a significant increase in the Cu content (by 89 %) 1 day after the ischemia modeling, and 2 days later – by a decrease in its content by 36 % (compared to the control). In the control animals that were not subjected to surgical operations, no changes in the content of NO or copper were observed. Conclusion. The experiments showed a 2-fold decrease in the NO content in the olfactory bulb of the rat brain 1 and 2 days after the ischemia modeling, and demonstrated that the intranasal administration of MSCs did not affect the intensity of NO production on the 1st and 2nd days after the brain ischemia modeling, but was accompanied by an increase in the antioxidant protection of the nervous tissue one day after ischemia.

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Changes of Nitric Oxide Content in the Rat Hippocampus, Heart and Liver in Acute Phase of Ischemia

Cited by 12 publications

References 52 publications

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Migration of Neural Stem Cells in Hippocampal Slices in Hypoxia Modeling

Participation of NO-synthase in Control of Nitric Oxide Level in Rat Hippocampus after Modelling of Ischaemic and Haemorrhagic Insult

Content of nitric oxide and copper in the olfactory bulbs of the rats brain after modeling of cerebral stroke and intranasal administration of mesenchymal stem cells

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