Epitope-targeted proteome analysis: towards a large-scale automated protein?protein-interaction mapping utilizing synthetic peptide arrays

Białek, Krzysztof; Swistowski, Andrzej; Frank, Ronald

doi:10.1007/s00216-003-1876-3

Cited by 33 publications

(18 citation statements)

References 20 publications

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“…For example, epitope specific antibodies can be isolated from polyclonal sera [28,29], thus, combining monospecificity with the ease of rabbit serum preparation. More recent novel options include, e.g., the preparation of miniprotein (protein domain) arrays by combination of solid phase synthesis and chemical ligation [30] or the multiplexed biopanning of phage libraries for genome wide protein interaction mapping [31].…”

Section: Other Protein Ligand Interactionsmentioning

confidence: 99%

Large-Scale Analysis of Protein–Protein Interactions Using Cellulose-Bound Peptide Arrays

Beutling

Städing

Stradal

et al. 2008

Protein – Protein Interaction

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Section: Other Protein Ligand Interactionsmentioning

confidence: 99%

Large-Scale Analysis of Protein–Protein Interactions Using Cellulose-Bound Peptide Arrays

Beutling

Städing

Stradal

et al. 2008

Protein – Protein Interaction

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“…Bialek et al described the development of a process for the genome-wide mapping of interactions between protein domains and peptide ligands entirely based on high-throughput microarray technology. [159] It is possible to attach a phage library displaying protein domains from a randomly fragmented and cloned complementary DNA (cDNA) library onto a peptide microarray; peptide-specific phage populations are then automatically eluted, propagated, labeled, and identified by hybridization to a DNA microarray after multiple enrichment. Takahashi et al constructed a novel protein-detection system in which 126 de novo peptides designed to form loop structures were labeled site-specifically with a fluorescent dye and immobilized into 96-well plastic plates.…”

Section: Peptide Microarraysmentioning

confidence: 99%

Protein‐Detecting Microarrays: Current Accomplishments and Requirements

2005

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The sequencing of the human genome has been successfully completed and offers the chance of obtaining a large amount of valuable information for understanding complex cellular events simply and rapidly in a single experiment. Interestingly, in addressing these proteomic studies, the importance of protein-detecting microarray technology is increasing. In the coming few years, microarray technology will become a significantly promising and indispensable research/diagnostic tool from just a speculative technology. It is clear that the protein-detecting microarray is supported by three independent but strongly related technologies (surface chemistry, detection methods, and capture agents). Firstly, a variety of surface-modification methodologies are now widely available and offer site-specific immobilization of capture agents onto surfaces in such a way as to keep the native conformation and activity. Secondly, sensitive and parallel detection apparatuses are being developed to provide highly engineered microarray platforms for simultaneous data acquisition. Lastly, in the development of capture agents, antibodies are now probably the most prominent capture agents for analyzing protein abundances. Alternative scaffolds, such as phage-displayed antibody and protein fragments, which provide the advantage of increasing diversity of proteinic capture agents, however, are under development. An approach involving recombinant proteins fused with affinity tag(s) and coupled with a highly engineered surface chemistry will provide simple production protocols and specific orientations of capture agents on the microarray formats. Peptides and other small molecules can be employed in screening highly potent ligands as well as in measuring enzymatic activities. Protein-detecting microarrays supported by the three key technologies should contribute in accelerating diagnostic/biological research and drug discovery.

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“…The authors "panned" a phage library displaying protein domains from a randomly fragmented and cloned cDNAlibrary on an array of synthetic peptide ligands. After multiplexed affinity enrichment, peptide specific phage populations will be automatically eluted, propagated, labeled and identified by hybridization to a DNA-microarray [153].…”

Section: Applicationsmentioning

confidence: 99%

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

Sobek¹,

Bartscherer²,

Jacob³

et al. 2006

CCHTS

110

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Over the last years microarray technology has become one of the principal platform technologies for the highthroughput analysis of biological systems. Starting with the construction of first DNA microarrays in the 1990s, microarray technology has flourished in the last years and many different new formats have been developed. Peptide and protein microarrays are now applied for the elucidation of interaction partners, modification sites and enzyme substrates. Antibody microarrays are envisaged to be of high importance for the high-throughput determination of protein abundances in translational profiling approaches. First cell microarrays have been constructed to transform microarray technology from an in vitro technology to an in vivo functional analysis tool. All of these approaches share a common prerequisite: the solid support on which they are generated. The demands on this solid support are thereby as manifold as the applications themselves. This review is aimed to display the recent developments in surface chemistry and derivatization, and to summarize the latest developments in the different application areas of microarray technology.

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Epitope-targeted proteome analysis: towards a large-scale automated protein?protein-interaction mapping utilizing synthetic peptide arrays

Cited by 33 publications

References 20 publications

Large-Scale Analysis of Protein–Protein Interactions Using Cellulose-Bound Peptide Arrays

Large-Scale Analysis of Protein–Protein Interactions Using Cellulose-Bound Peptide Arrays

Protein‐Detecting Microarrays: Current Accomplishments and Requirements

Microarray Technology as a Universal Tool for High-Throughput Analysis of Biological Systems

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