Epitope-Blocking Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Ross River Virus in Vertebrate Sera

Abstract

We describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) for the sensitive and rapid detection of antibodies to Ross River virus (RRV) in human sera and known vertebrate host species. This ELISA provides an alternative method for the serodiagnosis of RRV infections.

Nonstructural Protein 1-Specific Immunoglobulin M and G Antibody Capture Enzyme-Linked Immunosorbent Assays in Diagnosis of Flaviviral Infections in Humans

“…b Control panels were other serocomplex panels and negative panels. lished indirect ELISA, an epitope-blocking ELISA, or a complement-dependent cytotoxicity assay (29)(30)(31)(32), the use of an NS1-MAC-ELISA has not been reported, and validated test kits…”

Arthrogenic Alphaviruses and Inflammatory Myopathies

“…Like all alphaviruses, the virus is maintained in transmission cycles between its mosquito vector and vertebrate hosts. In the case of RRV the predominant vectors are the mosquitoes Culex annulirostris and Aedes vigilax and the vertebrate hosts are commonly found to be native marsupials (Harley et al, 2001;Old & Deane, 2005;Oliveira et al, 2006). In Australia, there are between 5,000 to 8,000 cases of RRV reported annually (Harley et al, 2001), with patients displaying symptoms of arthritis, arthralgia, myalgia, fatigue, febrile illness and rash (Fraser, 1986;Harley et al, 2002;Harley et al, 2001).…”

Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications

“…Scoring: +++++, OD.1.0; ++++, OD50.75-1.0; +++, OD50.5-0.75; ++, OD50.3-0.5; +, OD50.25-0.3. DmAb G8 was generated to the E1 protein of RRV and is cross-reactive with CHIKV and SFV (Oliveira et al, 2006). dmAb 2F2 was previously raised to the Australian prototype strain MRM39, and has been shown to be SINV-specific.…”

“…Expression of recombinant E1 (rE1) and E2 (rE2) of CHIKV MAU was also carried out for A cocktail of five mAbs generated in a previous study was used for the detection of CHIKV E2 (Goh et al, 2013). B10/G8 mAbs (anti-E1 cocktail) were generated to the E1 protein of RRV and are cross-reactive with the E1 protein of CHIKV (Oliveira et al, 2006). Successful expression of recombinant proteins was demonstrated using anti-V5 mAb.…”