Epithelial to mesenchymal transition is involved in ethanol promoted hepatocellular carcinoma cells metastasis and stemness

Chen, Danlei; Yu, Dandan; Wang, Xinyi; Liu, Yan; He, Yongjing; Deng, Ruiqing; Jiang, Yu; Zhang, Fengyun; Liu, Yakun; Xu, Mei; Li, Jiabin; Luo, Jia; Wang, Siying

doi:10.1002/mc.22850

Cited by 19 publications

(19 citation statements)

References 45 publications

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“…The pathological features of EMT include loss of cell-to-cell adhesion and cell polarity by epithelioid cells, which then transform into mesenchymal cells with increased proliferative, migratory, and invasive capacities. 23,24 In our study, western blot analysis revealed that overexpression of circRNA_100395 increased the expression of the epithelial marker, E-cadherin, and suppressed the mesenchymal proteins, N-cadherin, vimentin, and Slug, in PC3 and DU145 cells. Emerging studies have indicated that cell growth and metastasis are affected by EMT.…”

Section: Discussionsupporting

confidence: 50%

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Luo

et al. 2021

J Int Med Res

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Objective Circular RNAs (circRNAs) are non-coding RNAs with high cancer-specific expression and the potential for regulating tumorigenesis. CircRNA_100395 is expressed at low levels in many cancers and is involved in the regulation of tumor cell proliferation and metastasis. However, its expression and function in prostate cancer remain unclear. Methods Endogenous expression levels of circRNA_100395 and microRNA-1228 (miR-1228) in prostate cancer tissue samples and cell lines were detected by quantitative reverse transcription-polymerase chain reaction. Cell proliferation, invasion, and migration, cell cycle distribution, and epithelial–mesenchymal transition (EMT) were analyzed in circRNA_100395-overexpressing prostate cancer cells by Cell Counting Kit-8, flow cytometry, Transwell assay, and western blotting, respectively. Results CircRNA_100395 expression was downregulated in cancerous prostate tissues relative to adjacent normal tissues. CircRNA_100395 expression was negatively correlated with tumor size, Gleason score, tumor stage, and lymph node metastasis. Moreover, circRNA_100395 overexpression inhibited cell proliferation, altered cell cycle distribution, reduced cell migration and invasion abilities, and suppressed EMT in prostate cancer cells. Moreover, miR-1228 was a direct downstream target of circRNA_100395, and the anti-tumor ability of circRNA_100395 was significantly reversed by miR-1228. Conclusion This study identified circRNA_100395 as an anti-tumor circRNA and a potential therapeutic target for prostate cancer.

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Section: Discussionsupporting

confidence: 50%

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Luo

et al. 2021

J Int Med Res

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“…The transition of cells from epithelial to mesenchymal phenotype (EMT) enhances the migratory and invasive properties of cancerous cells and cause metastasis of malignant tumor 19,20. MiRNAs are reported to regulate EMT mediated metastasis 21–23.…”

Section: Discussionmentioning

confidence: 99%

<p>Downregulation of miR-216a-5p by long noncoding RNA PVT1 suppresses colorectal cancer progression via modulation of YBX1 expression</p>

Zeng¹,

Liu

Zhu³

et al. 2019

CMAR

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Purpose Increasing evidence has demonstrated that microRNAs (miRNAs) are closely related to the occurrence and development of tumors. MiR-216a-5p, located at 2p16.1, has been shown to suppress proliferation of cancerous cells. However, its expression and function in colorectal cancer (CRC) remain unclear. Materials and methods The significance of miR-216a-5p in CRC was studied by analyzing miR-216a-5p expression in CRC tissues and its association with clinicopathological parameters. CRC cells, stably overexpressing miR-216a-5p, were evaluated for cell proliferation and metastasis using cell counting kit-8 (CCK-8) and transwell assay methods. Epithelial–mesenchymal transition (EMT) pathway was analyzed by Western blotting. Bioinformatics, quantitative real-time polymerase chain reaction (RT-qPCR), and luciferase reporter assay were performed to define the regulation of PVT1/miR-216a-5p/Y Box Binding Protein 1 (YBX1) axis in CRC. Results The expression of miR-216a-5p was found to be significantly downregulated in CRC and was correlated with the various stages and differentiation degree of the tumors. Moreover, the overexpression of miR-216a-5p could significantly inhibit the tumor growth, metastasis, and EMT progression in CRC. Furthermore, the expression of miR-216a-5p was negatively correlated with the expression of PVT1, and PVT1 could reverse tumor suppressive effect of miR-216a-5p in CRC cells. Finally, YBX1 might be the key target of PVT1/miR-216a-5p axis in CRC. Conclusion Downregulation of miR-216a-5p by PVT1 could suppress CRC progression via modulating YBX1 expression.

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“…Epithelial-mesenchymal transition (EMT) is a biological process in which epithelioid cells lose cell-to-cell adhesion and cell polarity and then transform into mesenchymal cells with increased migratory and invasive abilities [23,24]. A growing number of studies indicate that circRNA inhibits cell metastasis via suppression of the EMT pathway [25,26].…”

Section: Discussionmentioning

confidence: 99%

Role of CircRNAs_100395 in Proliferation and Metastases of Liver Cancer

Chen

Cao

et al. 2019

Med Sci Monit

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Background Circular RNAs (circRNAs) are a kind of noncoding RNA with high cancer-specific expression, and great potential in regulating tumorigenesis. Among these, circRNA_100395 (circ_100395) has been reported to be downregulated in lung cancer, and participates in the process of tumor cell proliferation and metastasis. However, its expression and function in liver cancer remain unknown. Material/Methods Quantitative real-time polymerase chain reaction (RT-qPCR) was used to evaluate the expression level of circ_100395 and microRNAs-1228 (miR-1228) in liver cancer samples and the adjacent non-tumor tissues. Cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition (EMT) pathway of circ_100395 upregulated cells were analyzed using a Cell Counting Kit-8 (CCK-8), flow cytometry, Transwell assay, and Western blot analysis. Results We found that circ_100395 was downregulated in cancerous liver tissues relative to the adjacent normal tissues. The overexpression of circ_100395 was negatively associated with tumor differentiation, microvascular invasion, and portal vein tumor thrombosis. However, patients with higher circ_10039 expression tended to have better postoperative disease-free survival time. Moreover, upregulation of circ_100395 in liver cancer cells inhibited cell proliferation, induced apoptosis, then silenced the EMT pathway and reduced migration and invasion abilities, while this anti-tumor effect was significantly reversed by the downstream target, miR-1228. Conclusions circ_100395 appears to be a promising therapeutic target for liver cancer.

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Epithelial to mesenchymal transition is involved in ethanol promoted hepatocellular carcinoma cells metastasis and stemness

Cited by 19 publications

References 45 publications

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

<p>Downregulation of miR-216a-5p by long noncoding RNA PVT1 suppresses colorectal cancer progression via modulation of YBX1 expression</p>

Role of CircRNAs_100395 in Proliferation and Metastases of Liver Cancer

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