Role of CircRNAs_100395 in Proliferation and Metastases of Liver Cancer

Abstract

Background Circular RNAs (circRNAs) are a kind of noncoding RNA with high cancer-specific expression, and great potential in regulating tumorigenesis. Among these, circRNA_100395 (circ_100395) has been reported to be downregulated in lung cancer, and participates in the process of tumor cell proliferation and metastasis. However, its expression and function in liver cancer remain unknown. Material/Methods Quantitative real-time polymerase chain reaction (RT-qPCR) was used to evaluate the expression level of circ_100395 and microRNAs-1228 (miR-1228) in liver cancer samples and the adjacent non-tumor tissues. Cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition (EMT) pathway of circ_100395 upregulated cells were analyzed using a Cell Counting Kit-8 (CCK-8), flow cytometry, Transwell assay, and Western blot analysis. Results We found that circ_100395 was downregulated in cancerous liver tissues relative to the adjacent normal tissues. The overexpression of circ_100395 was negatively associated with tumor differentiation, microvascular invasion, and portal vein tumor thrombosis. However, patients with higher circ_10039 expression tended to have better postoperative disease-free survival time. Moreover, upregulation of circ_100395 in liver cancer cells inhibited cell proliferation, induced apoptosis, then silenced the EMT pathway and reduced migration and invasion abilities, while this anti-tumor effect was significantly reversed by the downstream target, miR-1228. Conclusions circ_100395 appears to be a promising therapeutic target for liver cancer.

Inhibitory role of circRNA_100395 in the proliferation and metastasis of prostate cancer cells

“…Emerging studies have indicated that cell growth and metastasis are affected by EMT. For example, Shao et al 25 found that NLRP3 promoted colorectal cancer cell proliferation and metastasis by regulating EMT, while Chen et al 14 showed that circRNA_100395 suppressed liver cancer cell proliferation and metastasis by regulating EMT. Collectively, these studies suggest that the mechanism underlying the antiproliferation and anti-metastatic effects of Accumulating studies have also indicated that circRNAs can interfere with the expression of miRNAs by functioning as miRNA sponges.…”

“…CircRNA_100395 is located at chr1: 173726114–17374498. 13 Emerging evidence has shown that circRNA_100395 is downregulated in tumors, and its expression pattern was strongly associated with lymph node metastasis, tumor size, and overall survival in patients with lung, 13 liver, 14 and ovarian cancers. 15 However, the relationship between circRNA_100395 and prostate cancer is still unclear.…”

circ_NOTCH3 Functions as a Protooncogene Competing With miR-205-5p, Modulating KLF12 Expression and Promoting the Development and Progression of Basal-Like Breast Carcinoma

“…circ_RNAs affect human disease, such as changes in the level of ciRS-7/CDR1as (the miR-7 circ_RNA sponge), and alter the levels of miR-7 target genes (both endogenous and reporter) ( 12 , 14 , 17 ). circ_RNAs also play a pivotal role in the pathogenesis and development of a diversity of cancers, such as liver ( 18 , 19 ), colon ( 20 , 21 ), gastric ( 22 , 23 ), lung ( 24 , 25 ), kidney cancer ( 26 , 27 ) and endometrial cancer ( 28 , 29 ).…”

Comprehensive Analysis of Differentially Expressed circRNAs Reveals a Colorectal Cancer-Related ceRNA Network

“…Currently, some researches had shown that circRNAs modulated alternative splicing and gene expression level through sponging microRNAs (miRNAs) [ 11 , 12 ]. At the same time, disorders of circRNAs took part in carcinogenesis and cancer development of CRC [ 13 , 14 ], liver cancer [ 15 ], and gastric cancer [ 16 – 19 ]. These findings indicated that circRNAs, as a new kind of endogenous noncoding RNA, have been a new hotspot in the cancer research and probably would exhibit important functions in the development of tumor.…”