Epithelial-stromal junction of normal and dysplastic mammary glands

Ozzello, Luciano

doi:10.1002/1097-0142(197003)25:3<586::aid-cncr2820250314>3.0.co;2-1

Cited by 70 publications

(17 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The relevance of directly exposing luminal cells to stromal fibroblasts from which they in situ are separated by myoepithelial cells and basement membrane could be questioned. However, while luminal cells at a glance may seem completely enveloped by myoepithelial cells, higher magnifications reveal that they project into the surrounding stroma containing delimiting fibroblasts [35]. The interstitial stroma in turn forms a barrier between capillaries and epithelium, across which epitheliotrophic stimuli from the blood supply must pass [36].…”

Section: Discussionmentioning

confidence: 99%

Evidence of two distinct functionally specialized fibroblast lineages in breast stroma

et al. 2016

View full text Add to dashboard Cite

BackgroundThe terminal duct lobular unit (TDLU) is the most dynamic structure in the human breast and the putative site of origin of human breast cancer. Although stromal cells contribute to a specialized microenvironment in many organs, this component remains largely understudied in the human breast. We here demonstrate the impact on epithelium of two lineages of breast stromal fibroblasts, one of which accumulates in the TDLU while the other resides outside the TDLU in the interlobular stroma.MethodsThe two lineages are prospectively isolated by fluorescence activated cell sorting (FACS) based on different expression levels of CD105 and CD26. The characteristics of the two fibroblast lineages are assessed by immunocytochemical staining and gene expression analysis. The differentiation capacity of the two fibroblast populations is determined by exposure to specific differentiating conditions followed by analysis of adipogenic and osteogenic differentiation. To test whether the two fibroblast lineages are functionally imprinted by their site of origin, single cell sorted CD271low/MUC1high normal breast luminal epithelial cells are plated on fibroblast feeders for the observation of morphological development. Epithelial structure formation and polarization is shown by immunofluorescence and digitalized quantification of immunoperoxidase-stained cultures.ResultsLobular fibroblasts are CD105high/CD26low while interlobular fibroblasts are CD105low/CD26high. Once isolated the two lineages remain phenotypically stable and functionally distinct in culture. Lobular fibroblasts have properties in common with bone marrow derived mesenchymal stem cells and they specifically convey growth and branching morphogenesis of epithelial progenitors.ConclusionsTwo distinct functionally specialized fibroblast lineages exist in the normal human breast, of which the lobular fibroblasts have properties in common with mesenchymal stem cells and support epithelial growth and morphogenesis. We propose that lobular fibroblasts constitute a specialized microenvironment for human breast luminal epithelial progenitors, i.e. the putative precursors of breast cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s13058-016-0769-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Evidence of two distinct functionally specialized fibroblast lineages in breast stroma

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This is probably due to the hemidesmosomes present along the basal plasmalemma of the myoepithelial cells. 4 The myoepithelial cells, however, are also firmly attached to the adjacent epithelial cells by desmosomes and gap junctions.5 The extraction of the parenchyma is associated with a shearing of the parenchyma at the level of the myoepithelial cell/basal lamina. This results in the removal of intact epithelial cells and the rupture of the myoepithelial cell plasmalemma and subsequent lysis.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural analysis of fine needle aspirates from benign breast lesions.

1990

View full text Add to dashboard Cite

“…Ultrastructurally, this basement membrane consists of an electron-lucent region, the lamina lucida, directly adject to the epithelial and myoepithelial cells, and an electron dense region, the lamina densa, located between the lamina lucida and the connective tissue (2). In some tissues anchoring fibrils that extend from the lamina densa into the surrounding connective tissue have been described (3).…”

mentioning

confidence: 99%

Topographical arrangement of basement membrane proteins in lactating rat mammary gland: comparison of the distribution of type IV collagen, laminin, fibronectin, and Thy-1 at the ultrastructural level.

Monaghan¹,

Warburton²,

Perusinghe³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The topographical distribution of type IV collagen, laminin, fibronectin, and the thymocyte differentiation antigen Thy-i in the basement membrane of the lactating rat mammary gland was investigated. Small cubes of tissue, which had not been subjected to prior fixation or freezing, were incubated with monospecific or monoclonal antibodies to these proteins, and the antibodies were located by an indirect immunoperoxidase staining technique and observed in the electron microscope. The lamina densa stained uniformly with antibodies to type IV collagen and laminin. In addition, both laminin and type IV collagen were present in semiperiodic clusters that traversed the lamina lucida from the cell surface to the lamina densa. Fibronectin was present only in the semiperiodic clusters and not elsewhere in the basement membrane. These clusters were irregularly spaced along the cell surface and heterogeneous in size. It remains to be determined if these three proteins are present in the same clusters. Thy-I was largely present on the lamina densa and not on the lamina lucida.The Thy-i staining of the lamina densa occurred in discrete maxima and minima. These maxima occurred in regions adjacent to Thy-l-bearing stromal cells. Thus, the topographical distribution of proteins within a basement membrane varies in a nonrandom manner, and local factors can modify this distribution. We suggest that this topographical variability may play a role in cell recognition and signalling processes that occur across the basement membrane.In the rodent and human mammary glands, the parenchyma are separated from the surrounding connective tissue by a basement membrane (1). Ultrastructurally, this basement membrane consists of an electron-lucent region, the lamina lucida, directly adject to the epithelial and myoepithelial cells, and an electron dense region, the lamina densa, located between the lamina lucida and the connective tissue (2). In some tissues anchoring fibrils that extend from the lamina densa into the surrounding connective tissue have been described (3). Several protein components of basement membranes have been identified (4). Type IV collagen appears to be restricted to the lamina densa (5-7), whereas laminin, fibronectin, and heparan sulfate proteoglycan are present in either the lamina lucida or lamina densa (5, 7-12). We have shown that antibodies to type IV collagen, laminin, and the thymocyte differentiation antigen Thy-1 stain the basement membrane in the mammary glands of both virgin and lactating rats at the light microscope level, whereas antibodies to fibronectin only stain the basement membrane in the mammary gland of lactating rats (1,13,14). We now report on the immunocytochemical distribution at the ultrastructural level of type IV collagen, laminin, fibronectin, and Thy-i in the basement membrane of the lactating rat mammary gland. METHODSAntibodies. Monospecific antibodies to type IV collagen and laminin, both purified from the mouse Engelbreth-Holm-Swarm (EHS) sarcoma, and rat plasma fibronectin hav...

show abstract

Epithelial-stromal junction of normal and dysplastic mammary glands

Cited by 70 publications

References 20 publications

Evidence of two distinct functionally specialized fibroblast lineages in breast stroma

Evidence of two distinct functionally specialized fibroblast lineages in breast stroma

Ultrastructural analysis of fine needle aspirates from benign breast lesions.

Topographical arrangement of basement membrane proteins in lactating rat mammary gland: comparison of the distribution of type IV collagen, laminin, fibronectin, and Thy-1 at the ultrastructural level.

Contact Info

Product

Resources

About