Ultrastructural analysis of fine needle aspirates from benign breast lesions.

Ferguson, David; Wells, Christopher; Crucioli, V.

doi:10.1136/jcp.43.1.22

Cited by 2 publications

(3 citation statements)

References 4 publications

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“…In all of the fibroadenoma lesions analyzed herein, we observed p63 positive NN admixed with sheets of epithelial cells. Our results are in accordance with early reports28–30 suggesting that NN have a myoepithelial origin and with the preliminary results of Barbareschi and colleagues 13. Their designation suggests that NN do not have intact cytoplasm.…”

Section: Discussionsupporting

confidence: 94%

Distribution of p63, a novel myoepithelial marker, in fine-needle aspiration biopsies of the breast

Reis‐Filho

Milanezi

Amendoeira

et al. 2003

Cancer

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A novel methodology for the de novo identification of peptides by mixed‐integer optimization and tandem mass spectrometry is presented in this article. The various features of the mathematical model are presented and examples are used to illustrate the key concepts of the proposed approach. Several problems are examined to illustrate the proposed method's ability to address (1) residue‐dependent fragmentation properties and (2) the variability of resolution in different mass analyzers. A preprocessing algorithm is used to identify important m/z values in the tandem mass spectrum. Missing peaks, resulting from residue‐dependent fragmentation characteristics, are dealt with using a two‐stage algorithmic framework. A cross‐correlation approach is used to resolve missing amino acid assignments and to identify the most probable peptide by comparing the theoretical spectra of the candidate sequences that were generated from the MILP sequencing stages with the experimental tandem mass spectrum. © 2006 American Institute of Chemical Engineers AIChE J 2007

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Section: Discussionsupporting

confidence: 94%

Distribution of p63, a novel myoepithelial marker, in fine-needle aspiration biopsies of the breast

Reis‐Filho

Milanezi

Amendoeira

et al. 2003

Cancer

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“…2,4 -7 Based on microscopic evaluations, Zajicek et al 6 and Linsk et al 2 suggested a myoepithelial origin for these cells, which was corroborated by the ultrastructural analysis done by Ferguson et al, 7 who also suggested that in FNAB the extraction process might result in a shearing between the epithelium and basal lamina, leading to myoepithelial cell lysis. On the other hand, Tsuchiya et al 4 suggested that NN were indeed derived from stromal cells, based on the lack of ATPase expression (a putative myoepithelial marker) and on the presence of ultrastructural features that are characteristically observed in stromal cells, such as abundant rough endoplasmic reticulum, numerous ribosomes, and lack of cytoplasmic filaments and dense bodies.…”

Section: Discussionmentioning

confidence: 84%

“…[1][2][3] Regardless of their important role in the diagnosis of benign lesions, the origin of NN remains disputable. 4 Most authors claim that NN derive from myoepithelial cells; 5,6,7 however, Tsuchiya et al, 4 based on ultrastructural features, suggested a stromal origin for these cells. This issue is far from being resolved, mainly due to methodological difficulties to address the question because no reliable nuclear myoepithelial cell marker was described until the last year.…”

mentioning

confidence: 97%

Naked nuclei revisited: p63 Immunoexpression

Reis‐Filho

Albergaria

Milanezi

et al. 2002

Diagnostic Cytopathology

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The presence of naked nuclei (NN) in cytological preparations of fibroadenomas is a well-known finding. Regardless of their importance on the differential diagnosis of fibroadenoma and other benign lesions of the breast, the origin of NN remains elusive. Despite previous efforts to characterize them, the lack of a reliable nuclear marker for myoepithelial cells impaired definitive conclusions. We performed a systematic evaluation of p63 expression in cytological and histological preparations of 10 fibroadenoma specimens. We observed that in histological sections, p63 was restricted to the nuclei of myoepithelial/basal cells in lobules and ducts of normal breast. In fibroadenomas, p63 decorated the nuclei of myoepithelial cells in the periphery of epithelial duct-like formations and slit-like formations. No p63 immunoreactivity was observed in stromal or epithelial cells. In cytological samples, almost all NN and cells surrounding epithelial cell clusters were stained; no stromal cell admixed with fibrillary matrix or epithelial cell was stained with p63. Based on our findings, we strongly suggest that most, if not all, NN are myoepithelial in origin.

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Ultrastructural analysis of fine needle aspirates from benign breast lesions.

Cited by 2 publications

References 4 publications

Distribution of p63, a novel myoepithelial marker, in fine-needle aspiration biopsies of the breast

Distribution of p63, a novel myoepithelial marker, in fine-needle aspiration biopsies of the breast

Naked nuclei revisited: p63 Immunoexpression

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