Epithelial–stromal interactions in salivary glands of rats exposed to chronic passive smoking

Ferragut, Juliana Mandato; Cunha, Marcelo Rodrigues da; Carvalho, Cesar Alexandre Fabrega; Isayama, Ricardo Noboro; Caldeira, Eduardo José

doi:10.1016/j.archoralbio.2010.11.017

Cited by 19 publications

(19 citation statements)

References 44 publications

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“…Tobacco use results in local exposure of the oral mucosa to nicotine and the heavy metals present in it. Due to the release of nicotine from the mucous membranes and absorption of swallowed nicotine, its continuous absorption takes place even after the tobacco is discarded from the mouth; therefore, it can diffuse from the site of generation and inflict damage at remote locations (29,30). With continuous local irritation by tobacco and the genotoxic effects of nicotine accumulation in saliva, injuries related to chronic inflammation and OS occurs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma

Metgud

Bajaj

2014

J Oral Sci

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Lipid peroxidation induced by reactiveoxygen species (ROS) is involved in the pathogenesis of malignancy. Overall, lipid peroxidation levels are indicated by malondialdehyde (MDA), which is the most frequently used biomarker to detect oxidative changes. Antioxidant defense systems such as glutathione (GSH) limit cell injury induced by ROS. Therefore, MDA and GSH can be used to monitor oxidative stress (OS). Hence, this study aimed to evaluate and compare both salivary and serum levels of MDA and GSH in oral leukoplakia and oral squamous cell carcinoma (OSCC) patients, and healthy controls. The study included 100 subjects comprising 30 apparently healthy controls, 30 patients with oral leukoplakia and 40 clinically and histologically diagnosed patients with OSCC. Saliva and blood samples were obtained and evaluated for MDA and GSH. The study revealed enhanced MDA levels in saliva and serum in oral leukoplakia and OSCC patients as compared to controls. On the other hand, significant decreases were seen in serum and salivary GSH levels in oral leukoplakia and OSCC patients as compared to controls. Augmentation of OS in blood and saliva is reflected by increase in MDA and decrease in GSH levels, indicating that tumor processes cause an imbalance of oxidant-antioxidant status in cell structures.(J Oral Sci 56, 135-142, 2014)

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Section: Discussionmentioning

confidence: 99%

Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma

Metgud

Bajaj

2014

J Oral Sci

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“…Therefore, the high density of collagen and elastic fibers found in the present study may be due in part to androgen depletion as indicated by the low circulating testosterone levels observed in the nicotine group. Similarly, a recent study investigating the effects of passive smoking also demonstrated a significant accumulation of type I collagen fibers (Ferragut et al,2010). Moreover, the morphological characteristics and distribution of collagen I observed in the present study differed from the findings obtained for androgen deprivation in rats (Vilamaior et al,2000).…”

Section: Discussionmentioning

confidence: 75%

Morphological alterations in the prostate stroma of rats submitted to chronic nicotine treatment

Carvalho

Pissolato

Cândido

et al. 2012

Microscopy Res & Technique

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The stroma plays a fundamental role in the function of different glandular systems. In the prostate, the stroma is responsible for the development and maintenance of the differentiated state of the epithelium. Nicotine induces tobacco dependence and promotes morphological alterations in the epithelial compartment. However, its effects on the prostate stroma are unclear. The objective of this study was to evaluate the morphology of the stromal microenvironment in the ventral prostate lobe of rats submitted to chronic nicotine administration. Twenty rats (Rattus norvegicus) were divided into two groups: 10 animals received subcutaneous nicotine and 10 animals received physiological saline by the same route. After treatment, samples were collected from the ventral prostate lobe, processed and submitted to histology, histochemistry, and ultrastructural analysis by transmission and scanning electron microscopy. The level of circulating testosterone was also analyzed. The results showed a significant increase in the density of type I collagen (56.3% to 85.9%, P < 0.01) and a decrease in the density of type III collagen (43.7% to 14.1%, P < 0.01). In addition, there was a qualitative increase in elastic fibers and in the number of smooth muscle cells with a secretory phenotype. Circulating testosterone levels were significantly reduced (898.3 to 363.1 ng/mL, P < 0.01). The results showed that nicotine modifies different components of the prostate stroma, suggesting that this drug is a risk factor for morphofunctional alterations in the prostate gland.

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“…In this sense, some studies relate that patients with hernias in the abdomen wall also have diabetes, associated or not with the use of tobacco or alcohol. However, these are studies that only focus these elements as being risk factors for the occurrence of hernias, but do not explain which changes happen in the tissues 12 , 16 .…”

Section: Discussionmentioning

confidence: 99%

“…At these storages there are the connective tissues that at normal conditions present themselves among the cells, thus structuring the organ´s construction, as well as providing subsidies for its maintenance 12 , 21 , 22 , 26 , 33 .…”

Section: Discussionmentioning

confidence: 99%

“…All the tissues, including the muscle ones, hold a support compartment, which owns blood, lymphatic vessels and nerves, presenting also connective tissues in which the collagen type is the predominant one, even though other types are also present. At normal environments, the collagen is regular among the cells; however, at adverse conditions these components can be increased 12 , 16 , 20 . …”

Section: Introductionmentioning

confidence: 99%

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Influence of Tobacco, Alcohol and Diabetes on the Collagen of Cremaster Muscle in Patients With Inguinal Hernias

Modena

Caldeira

Peres

et al. 2016

ABCD, arq. bras. cir. dig.

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Background: New findings point out that the mechanism of formation of the hernias can be related to the collagenous tissues, under activity of aggressive agents such as the tobacco, alcohol and diabetes. Aim: To analyze the collagen present in the cremaster muscle in patients with inguinal hernias, focusing the effect of tobacco, alcohol, and diabetes. Methods: Fifteen patients with inguinal hernia divided in three groups were studied: group I (n=5) was control; group II (n=5) were smokers and/or drinkers; and group III (n=5) had diabetes mellitus. All subjects were underwent to surgical repair of the inguinal hernias obeying the same pre, intra and postoperative conditions. During surgery, samples of the cremaster muscle were collected for analysis in polarized light microscopy, collagen morphometry and protein. Results: The area occupied by the connective tissue was higher in groups II and III (p<0.05). The collagen tissue occupied the majority of the samples analyzed in comparison to the area occupied by muscle cells. The content of total protein was higher in groups II and III compared to the control group (p<0.05). Conclusion: The tobacco, alcohol and diabetes cause a remodel the cremaster muscle, leading to a loss of support or structural change in this region, which may enhance the occurrences and damage related to inguinal hernias.

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Epithelial–stromal interactions in salivary glands of rats exposed to chronic passive smoking

Abstract: Passive smoking led to substantial structural changes in the salivary glands which could significantly affect function.

Cited by 19 publications

References 44 publications

Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma

Evaluation of salivary and serum lipid peroxidation, and glutathione in oral leukoplakia and oral squamous cell carcinoma

Morphological alterations in the prostate stroma of rats submitted to chronic nicotine treatment

Influence of Tobacco, Alcohol and Diabetes on the Collagen of Cremaster Muscle in Patients With Inguinal Hernias

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