Epithelial mesenchymal transition and cancer stem cells in esophageal adenocarcinoma originating from Barrett’s esophagus

Tomizawa, Yutaka; Wu, Tsung Teh; Wang, Kenneth K.

doi:10.3892/ol.2012.632

Cited by 27 publications

(25 citation statements)

References 34 publications

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“…Some of these repressors are specifically recognized at the invasive front of carcinogenesis [50,51]. Hotz et al used immunohistochemistry to examine the expression of these transcription factors in tissue samples from patients with pancreatic ductal adenocarcinoma [52].…”

Section: Molecular Signals During the Emtmentioning

confidence: 98%

The epithelial to mesenchymal transition in pancreatic cancer: A systematic review

et al. 2015

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Section: Molecular Signals During the Emtmentioning

confidence: 98%

The epithelial to mesenchymal transition in pancreatic cancer: A systematic review

et al. 2015

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“…Second some additional reports have implicated endodermal (including epithelial) embryonic markers in man but their relevance to human stem cells remains unclear 16 . Third other 'markers' have also been implicated in the epithelial mesenchymal transition of BE cells as they progress to cancer 17 . It remains unclear therefore whether these markers actually label stem cells or relate more to cancer stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…Despite these problems other techniques which trace lineage by genetic analyses strongly favour the likelihood of common stem cells between squamous and columnar esophageal mucosa [19][20][21][22][23][24] . The site of stem cells in BE has been more problematic [10][11][12][13][14][15] as well as the availability of true 'biomarkers' of stem cells [16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

et al. 2013

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Background & Aims:The existence of slowly-cycling, adult stem cells has been challenged by the identification of actively cycling cells. We investigated the existence of uncommitted, slowly cycling cells by tracking 5-iodo-2'-deoxyuridine (IdU) label-retaining cells (LRCs) in normal esophagus, Barrett's esophagus (BE), esophageal dysplasia, adenocarcinoma, and healthy stomach tissues from patients.Methods: Four patients (3 undergoing esophagectomy, 1 undergoing esophageal endoscopic mucosal resection for dysplasia and an esophagectomy for esophageal adenocarcinoma) received intravenous infusion of IdU (200 mg per m 2 body surface area, maximum dose of 400 mg) over a 30-min period; the IdU had a circulation t 1/2 of 8hs. Tissues were collected at 7, 11, 29 and 67 days following infusion, from regions of healthy esophagus, BE, dysplasia, adenocarcinoma, and healthy stomach; they were analyzed by in situ hybridization, flow cytometry, and immunohistochemical analyses. Results:No LRCs were found in dysplasias or adenocarcinomas, but there were significant numbers of LRCs in the base of glands from BE tissue, in the papillae of the basal layer of the esophageal squamous epithelium, and in the neck/isthmus region of healthy stomach. These cells cycled slowly, because IdU was retained for at least 67 days and co-labeling with Ki-67 was infrequent. In glands from BE tissues, most cells did not express defensin-5, Muc-2, or chromogranin A, indicating that they were not lineage committed. Some cells labeled for endocrine markers and IdU at 67 days; these cells represented a small population (<0.1%) of epithelial cells at this timepoint. The epithelial turnover time of the healthy esophageal mucosa was approximately 11 days (twice that of the intestine). Conclusions:LRCs of human esophagus and stomach have many features of stem cells (long lived, slow cycling, uncommitted, and multipotent), and can be found in a recognized stem cell niche. Further analyses of these cells, in healthy and metaplastic epithelia is required.4

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“…For statistical analysis, samples were categorized into 2 groups: negative and weakly positive cases versus strongly positive cases. 21 …”

Section: Immunohistochemistrymentioning

confidence: 99%

TWIST1 is a molecular marker for a poor prognosis in oral cancer and represents a potential therapeutic target

Silva

Alaoui‐Jamali

Soares

et al. 2013

Cancer

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BACKGROUND: Locoregional recurrence and distant metastases are ominous events in patients with advanced oral squamous cell carcinoma (OSCC). The objective of this study was to identify functional biomarkers that are predictive of OSCC progression to metastasis. METHODS: The expression profile of a network of epithelial-mesenchymal transition (EMT) genes was investigated in a large cohort of patients with progressive OSCC using a complimentary DNA microarray platform coupled to quantitative reverse transcriptase-polymerase chain reaction and immunohistochemical analyses. Therapeutic potential was investigated in vitro and in vivo using an orthotopic mouse model of metastatic OSCC growing in the tongue microenvironment. RESULTS: Among deregulated EMT genes, the Twist-related protein 1 (TWIST1) transcription factor and several of its regulated genes were significantly overexpressed across advanced stages of OSCC. This result was corroborated by the clinical observation that Twist1 up-regulation predicted the occurrence of lymph node and lung metastases as well as poor patient survival. In support of Twist1 as a driver of OSCC progression, the up-regulation of Twist1 was observed in cells isolated from patients with metastatic OSCC. The inhibition of Twist1 in these metastatic cells induced a potent inhibition of cell invasiveness in vitro as well as progression in vivo. CONCLUSIONS: The current results provide evidence for the prognostic value and therapeutic potential of a network of Twist genes in patients with advanced OSCC. Cancer 2014;120:352-62.

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Epithelial mesenchymal transition and cancer stem cells in esophageal adenocarcinoma originating from Barrett’s esophagus

Cited by 27 publications

References 34 publications

The epithelial to mesenchymal transition in pancreatic cancer: A systematic review

The epithelial to mesenchymal transition in pancreatic cancer: A systematic review

Identification of Lineage-Uncommitted, Long-Lived, Label-Retaining Cells in Healthy Human Esophagus and Stomach, and in Metaplastic Esophagus

TWIST1 is a molecular marker for a poor prognosis in oral cancer and represents a potential therapeutic target

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