Epithelial Cell Proliferation, Apoptosis, and Apoptosis Inhibition in Inverted Papillomas

Guichard, Cécile; Gilain, L.; Samad, Idris; Piron, Gaelle; Brugel, L.; Escudier, Estelle; Coste, A.

doi:10.1097/00005537-199805000-00017

Cited by 25 publications

(23 citation statements)

References 27 publications

(62 reference statements)

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“…Findings regarding the incidence percentage of HPV with a variable degree of dysplasia [Zhou et al, 2001] also appear to contradict the results of the present study. A higher degree of proliferation and apoptosis has been demonstrated in inverted papilloma compared to nasal polyps [Guichard et al, 1998]. Cellular proliferation was also significantly higher than apoptosis in the inverted papilloma, which is characteristic of their neoplastic structure.…”

Section: Discussionmentioning

confidence: 98%

Prevalence of human papilloma virus and human herpes virus types 1–7 in human nasal polyposis

Zaravinos

Bizakis

Spandidos

2009

Journal of Medical Virology

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This study aimed to investigate the prevalence of human papilloma virus (HPV), herpes simplex virus-1/-2 (HSV-1/-2), varicella-zoster virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpes virus-6/-7 (HHV-6/-7) in 23 human nasal polyps by applying PCR. Two types of control tissues were used: adjacent inferior/middle turbinates from the patients and inferior/middle turbinates from 13 patients undergoing nasal corrective surgery. EBV was the virus most frequently detected (35%), followed by HPV (13%), HSV-1 (9%), and CMV (4%). The CMV-positive polyp was simultaneously positive for HSV-1. HPV was also detected in the adjacent turbinates (4%) and the adjacent middle turbinate (4%) of one of the HPV-positive patients. EBV, HSV, and CMV were not detected in the adjacent turbinates of the EBV-, HSV- or CMV-positive patients. All mucosae were negative for the VZV, HHV-6, and HHV-7. This is the first study to deal with the involvement of a comparable group of viruses in human nasal polyposis. The findings support the theory that the presence of viral EBV markedly influences the pathogenesis of these benign nasal tumors. The low incidence of HPV detected confirms the hypothesis that HPV is correlated with infectious mucosal lesions to a lesser extent than it is with proliferative lesions, such as inverted papilloma. The low incidence of HSV-1 and CMV confirms that these two herpes viruses may play a minor role in the development of nasal polyposis. Double infection with HSV-1 and CMV may also play a minor, though causative, role in nasal polyp development. VZV and HHV-6/-7 do not appear to be involved in the pathogenesis of these mucosal lesions.

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Section: Discussionmentioning

confidence: 98%

Prevalence of human papilloma virus and human herpes virus types 1–7 in human nasal polyposis

Zaravinos

Bizakis

Spandidos

2009

Journal of Medical Virology

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“…In contrast, sinunasal inverted papillomas histologically show a hyperplasia of the epithelium itself, sometimes even with a varying degree of dysplasia. Furthermore, comparing nasal polyps and inverted papillomas, Guichard et al (24) demonstrated that there is a signi® cantly higher rate of proliferation as well as apoptosis in the latter. Concerning inverted papillomas, cell proliferation was also signi® cantly higher than apoptosis, characterizing their neoplastic structure.…”

Section: Discussionmentioning

confidence: 99%

Tracing Human Papillomavirus DNA in Nasal Polyps by Polymerase Chain Reaction

Hoffmann

Kahn

Goeroegh

et al. 2000

Acta Oto-Laryngologica

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Human papillomavirus (HPV) infections are related to the genesis of various benign and malignant human neoplasias. The HPV types 16 and 18 seem to be causally related to the development of most squamous cell carcinoma of the anogenital tract and a proportion of carcinomas of the upper aerodigestive tract. The near 100% positivity of the HPV types 6 and 11 in laryngeal papillomatosis is well established. We investigated whether HPV also plays a role in non-neoplastic mucosal entities such as sinunasal polyposis, the genesis of which has been discussed as being triggered by viral infections. On DNA from 39 sinunasal polyps (33 patients), polymerase chain reaction (PCR) was performed using beta-globin primers for demonstration of amplifiable DNA in the tissue extracts. Consensus primers for the detection of several different HPV types were applied to the beta-globin-positive samples. The results were confirmed by Southern blot hybridization using consensus probes. Cycle sequencing was performed on the positive cases. All 39 samples showed positive signals for beta-globin. HPV-DNA investigations showed a slight positive signal in only 1 of the 39 investigated cases (2.6%). Further molecular investigations of this sample, including cycle sequencing, could not confirm this result. All the other tissue samples remained HPV-DNA-negative. Therefore, those HPV types readily detectable with the PCR primers and probes used are not frequently associated with sinunasal polyposis. The data confirm the hypothesis that HPV is correlated to a lesser extent to infectious mucosal lesions than to proliferative lesions. Furthermore, the results emphasize that the presence of HPV in specific lesions does not occur by chance, but represents a specific infection of the mucosa leading to proliferation and even to malignancy.

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“…1 These tumours are characterized by hyperplastic epithelium with an endophytic growth pattern, while the basement membrane is intact and morphologically normal. 2 Nasal polyps are abnormal lesions that originate from any portion of the nasal mucosa or paranasal sinuses.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Analysis of PCNA, Ki67 and p53 in Nasal Polyposis and Sinonasal Inverted Papillomas

et al. 2007

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Immunoreactivity of proliferating cell nuclear antigen (PCNA), Ki67 and p53 in inflammatory nasal polyp and inverted papilloma tissues was investigated. Immunohistochemistry was performed using a standard avidin-biotin-peroxidase method, and the immunoreactivity of PCNA, Ki67 and p53 was quantified by counting immunostained nuclei in at least 1000 epithelial cells. The mean labelling index (percentage of immunostained cells) for PCNA was 40.68 in the inverted papilloma group and 14.73 in the nasal polyp group, and for Ki67 was 15.43 in the inverted papilloma group and 2.64 in the nasal polyp group. Both of these differences between the inverted papilloma and nasal polyp groups were significant. Immunoreactivity for p53 was detected in five (35.7%) inverted papilloma patients and two (9.5%) nasal polyp patients. The increase in epithelial cell proliferation seemed to be greater in inverted papillomas than in inflammatory nasal polyps. Increased epithelial cell proliferation may be involved in the development of inverted papillomas.

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Epithelial Cell Proliferation, Apoptosis, and Apoptosis Inhibition in Inverted Papillomas

Cited by 25 publications

References 27 publications

Prevalence of human papilloma virus and human herpes virus types 1–7 in human nasal polyposis

Prevalence of human papilloma virus and human herpes virus types 1–7 in human nasal polyposis

Tracing Human Papillomavirus DNA in Nasal Polyps by Polymerase Chain Reaction

Immunohistochemical Analysis of PCNA, Ki67 and p53 in Nasal Polyposis and Sinonasal Inverted Papillomas

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