Epithelial cell biocompatibility of silica nanospheres for contrast-enhanced ultrasound molecular imaging

Chiriacò, Fernanda; Conversano, Francesco; Soloperto, Giulia; Casciaro, Ernesto; Ragusa, Andrea; Sbenaglia, E. A.; Dipaola, Lucia; Casciaro, Sergio

doi:10.1007/s11051-013-1779-y

Cited by 26 publications

(22 citation statements)

References 47 publications

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“…The SPION@-Au core-shells can then be coated with methylpolyethylene glycol (mPEG) to prevent or reduce recognition of the core-shell by the reticuloendothelial system, improving their circulation properties. 21,22 To examine the potential of core-shells as MR contrast agents, the LNCap prostate cancer line 23 was selected to induce human prostate tumors in nude mice as a xenograft model.…”

Section: Introductionmentioning

confidence: 99%

Development of Gold-Coated Magnetic Nanoparticles as a Potential MRI Contrast Agent

Montazerabadi

Oghabian

Irajirad

et al. 2015

NANO

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Gold-coated superparamagnetic iron oxide nanoparticles (SPIONs) coated with methylpolyethylene glycol (mPEG) are synthesized and investigated as a magnetic resonance (MR) imaging contrast agent. The synthesized mPEG-core@shells are characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), vibrating sample magnetometry (VSM), zeta-potential analysis and X-ray di®raction (XRD). In addition, the transverse relaxivity of the mPEG-core@shells is measured using a 3 T MRI scanner. The cytotoxicity of the mPEG-core@shells is tested in the LNCaP cell line using an 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results show that the mPEG-core@shell particles are semispherical with hydrodynamic size of $ 65 nm and a transverse relaxivity of 162.3 mM À1 S À1 . The mPEG-core@shell particles demonstrate good stability in biological media without any signi¯cant in vitro cytotoxicity under high cellular uptake conditions. Finally, in vivo imaging shows that mPEG-core@shells are a potential contrast agent for use in early-stage detection.

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Section: Introductionmentioning

confidence: 99%

Development of Gold-Coated Magnetic Nanoparticles as a Potential MRI Contrast Agent

Montazerabadi

Oghabian

Irajirad

et al. 2015

NANO

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“…The adopted NP amount corresponded to a 0.2% volume concentration, and this value was chosen to stay in the range 1.5-5 mg/mL, for which it has been recently demonstrated that PEG-coated SiNSs in our considered size range have a high biocompatibility level [22]. Three different phantoms were prepared (one for each considered SiNS size), where the adopted NP volume concentration corresponded to the following number concentrations: 1) 9.0 · 10 11 NPs/mL for 160-nm SiNSs (first phantom); 2) 1.1 · 10 11 NPs/mL for 330-nm SiNSs (second phantom); and 3) 1.3 · 10 10 NPs/mL for 660-nm SiNSs (third phantom), respectively.…”

Section: Phantom Preparationmentioning

confidence: 99%

“…These issues are addressed in this paper, in which the employed SiNSs were coated with a layer of polyethylene glycol (PEG), known for its biocompatibility, and the adopted NP dose was reduced to stay within 5 mg/mL, since epithelial cell biocompatibility has been recently demonstrated up to this limit for the same NPs [22]. Furthermore, a different phantom configuration, based on the employment of Eppendorf tubes in place of Petri dishes, was used to assess possible effects of different target geometries on method validity.…”

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confidence: 99%

An Innovative Ultrasound Signal Processing Technique to Selectively Detect Nanosized Contrast Agents in Echographic Images

Casciaro

Pisani

Soloperto

et al. 2015

IEEE Trans. Instrum. Meas.

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The aim of this paper was to optimize the employment of a novel algorithm for acquisition and processing of medical ultrasound (US) signals to facilitate its clinical translation. The implemented procedure is dedicated to selective enhancement of nanoparticle (NP) contrast agents in echographic images and is based on the differences in US signal backscatter between NP-containing targets and more homogeneous objects. Previous preliminary studies verified the feasibility of this approach on silica nanospheres (SiNSs) dispersed at a constant volume concentration (0.7%) in agarose gel samples. The present extended these evaluations, addressing two issues of direct clinical interest: 1) safety: SiNSs were coated with a biocompatible layer made of polyethylene glycol (PEG) and the adopted NP volume concentration was reduced to 0.2%, which is in the nontoxic range and 2) reproducibility: a different phantom configuration was used, to verify the independence of algorithm performance from a specific target region shape. The obtained results demonstrated that the proposed method can be effectively applied to enhance the presence of PEG-coated SiNSs in the diameter range 160-660 nm at a low and biocompatible volume concentration: the combined employment of a phantom with a different geometry and a lower concentration of PEG-coated NPs, in fact, caused only slight variations in the suppression patterns of noncontrast echoes, without affecting the final diagnostic effectiveness of the investigated contrast detection scheme. This approach also provides specific advantages with respect to the available measurement techniques dedicated to the enhancement of targeted US contrast agents for molecular imaging purposes.Index Terms-Medical imaging, molecular imaging, nanoparticles (NPs), ultrasound (US) contrast agents, US signal and image processing.

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“…Recently, nanotechnology has been shown to be able to provide valuable tools for potentially diagnosing and treating diseases in many fields of medicine, including NDs [4][5][6][7][8][9][10]. Furthermore, NPs can help to overcome several limitations of traditional drugs by improving their bioavailability and half-life, yielding a sustained release, in addition to allowing specific targeting [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Investigation of Chitosan Nanoparticles for Dopamine Delivery

et al. 2018

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Featured Application: Chitosan nanoparticles are potential candidates for the delivery of various drugs, including dopamine.Abstract: Chitosan nanoparticles (CS NPs) have been widely exploited for the delivery of various types of drugs due to their biocompatibility, availability, ease of functionalization and other advantages. Nevertheless, despite their wide use, their mechanism of action is not very clear and many aspects still need to be investigated in detail, with only a few studies having studied the behavior of this polymer. We prepared CS NPs encapsulating dopamine (DA) and studied the generation of reactive oxygen species (ROS) and the antioxidant effect of the neurotransmitter in detail. Encapsulation of the drug and its subsequent sustained release significantly reduced the oxidation rate in vitro, thus potentially exerting neuroprotective effects. ROS production in SH-SY5Y cells was investigated through a H 2 O 2 assay, while a deeper study of the enzymatic activity allowed us to determine the significant contribution of both GPx and SOD enzymes in preventing oxidative stress.

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Epithelial cell biocompatibility of silica nanospheres for contrast-enhanced ultrasound molecular imaging

Cited by 26 publications

References 47 publications

Development of Gold-Coated Magnetic Nanoparticles as a Potential MRI Contrast Agent

Development of Gold-Coated Magnetic Nanoparticles as a Potential MRI Contrast Agent

An Innovative Ultrasound Signal Processing Technique to Selectively Detect Nanosized Contrast Agents in Echographic Images

Neuroprotective Investigation of Chitosan Nanoparticles for Dopamine Delivery

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