Epithelial cell adhesion molecule independent capture of non-small lung carcinoma cells with peptide modified microfluidic chip

Pu, Kefeng; Li, Chunlin; Zhang, Nengpan; Wang, Hui; Shen, Wenjiang; Zhu, Y.

doi:10.1016/j.bios.2016.09.092

Cited by 18 publications

(15 citation statements)

References 27 publications

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“…Pu et al 73 developed the A-1 peptide, which could capture both epithelial-like and mesenchymal-like A549 cells ( Figure 5A ). The A-1 peptide was obtained by bacterial surface display method and flow activated cell sorting (FACS) technology.…”

Section: Current Key Challenges and Corresponding Methodsmentioning

confidence: 99%

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Advances in isolation and detection of circulating tumor cells based on microfluidics

Zou

Cui

2018

Cancer Biology & Medicine

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Circulating tumor cells (CTCs) are the cancer cells that circulate in the peripheral blood after escaping from the original or metastatic tumors. CTCs could be used as non-invasive source of clinical information in early diagnosis of cancer and evaluation of cancer development. In recent years, CTC research has become a hotspot field wherein many novel CTC detection technologies based on microfluidics have been developed. Great advances have been made that exhibit obvious technical advantages, but cannot yet satisfy the current clinical requirements. In this study, we review the main advances in isolation and detection methods of CTC based on microfluidics research over several years, propose five technical indicators for evaluating these methods, and explore the application prospects. We also discuss the concepts, issues, approaches, advantages, limitations, and challenges with an aim of stimulating a broader interest in developing microfluidics-based CTC detection technology.

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Section: Current Key Challenges and Corresponding Methodsmentioning

confidence: 99%

“…(A) Microfluidic chip modified with NeutrAvidin and subsequently linked with biotin conjugated A-1 peptide from ref. 73 with permission from Biosensors and Bioelectronics, copyright 2017. (B) The dual-patterned chips from ref.…”

Section: Current Key Challenges and Corresponding Methodsmentioning

confidence: 99%

Advances in isolation and detection of circulating tumor cells based on microfluidics

Zou

Cui

2018

Cancer Biology & Medicine

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“…The binding between EpCAM and the anti-EpCAM antibody coated on the microdevice channels is highly specific, however only enables to capture the CTCs that possess epithelial phenotype [120]. Such an approach is not appropriate for CTCs isolation that undergo an EMT process or express mesenchymal phenotype [121, 122]. More comprehensive applications exploit antibodies against the leucocyte antigen CD45 [123], called negative separation.…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

“…The development of affinity-based techniques results in the improvement of the efficiency of CTCs separation from other components of blood and creation of the opportunity for cells segregation based on the abundance of surface markers expression [117, 130, 131]. This progress is possible among others through magnetic affinity-selection achieved by magnetic ranking cytometry (MagRC) [111, 132] and employment of magnetic items such as beads [17, 131, 133] and nanoparticles [17, 117, 130, 134, 135] coated with antibodies [131, 134], aptamers [17, 130] and peptides [121]. Employment of magnetic items permits the purification achievement by target cells gathering via a magnetic field [17], or by application of a magnetic sorter [131].…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

“…Several microfluidic systems have already been employed for lung metastasis screening of CTCs (Tables 2 and 3). They utilize anti-EpCAM antibody conjugation to magnetic upconversion of nanoparticles for CTCs capture [109], as well as anti-EpCAM substitution with A-1 peptide for different types of CTCs entrapment including those undergoing EMT process [121]. Parallel pre-EMT and post-EMT cells capture was also proven possible using nanoroughened glass [173].…”

Section: Circulating Tumor Cellsmentioning

confidence: 99%

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Microfluidics for studying metastatic patterns of lung cancer

et al. 2019

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The incidence of lung cancer continues to rise worldwide. Because the aggressive metastasis of lung cancer cells is the major drawback of successful therapies, the crucial challenge of modern nanomedicine is to develop diagnostic tools to map the molecular mechanisms of metastasis in lung cancer patients. In recent years, microfluidic platforms have been given much attention as tools for novel point-of-care diagnostic, an important aspect being the reconstruction of the body organs and tissues mimicking the in vivo conditions in one simple microdevice. Herein, we present the first comprehensive overview of the microfluidic systems used as innovative tools in the studies of lung cancer metastasis including single cancer cell analysis, endothelial transmigration, distant niches migration and finally neoangiogenesis. The application of the microfluidic systems to study the intercellular crosstalk between lung cancer cells and surrounding tumor microenvironment and the connection with multiple molecular signals coming from the external cellular matrix are discussed. We also focus on recent breakthrough technologies regarding lab-on-chip devices that serve as tools for detecting circulating lung cancer cells. The superiority of microfluidic systems over traditional in vitro cell-based assays with regard to modern nanosafety studies and new cancer drug design and discovery is also addressed. Finally, the current progress and future challenges regarding printable and paper-based microfluidic devices for personalized nanomedicine are summarized. Electronic supplementary material The online version of this article (10.1186/s12951-019-0492-0) contains supplementary material, which is available to authorized users.

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Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

Yuan,

Chen,

Wan

et al. 2024

Adv Materials Technologies

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Cancer is now a major threat to human health and life. Although a number of clinical approaches to cancer treatment have been developed in recent years, tumor recurrence and metastasis remain a challenge. As the “seed” for the formation of tumor metastasis, Circulating Tumor Cells (CTCs) can not only grow and spread in the primary site, but also spread to other parts of the body through various ways. The close connection between CTCs and tumor progression makes them a focus of clinical and basic research attention, and it not only has an important guide for the clinical treatment of patients, but also a window into the occurrence and development of distant metastases. The identification and detection of CTCs have an important clinical significance in the assessment of the prognosis of cancer, efficacy prediction, efficacy evaluation, and the study of recurrent metastasis and drug resistance mechanisms of cancer patients. Therefore, this review not only introduces the source, attributes, transfer, and harm of CTCs, but also highlights the progress and latest technologies for the identification and capture and elimination strategies of CTCs and presents prospects for future research of CTCs.

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Epithelial cell adhesion molecule independent capture of non-small lung carcinoma cells with peptide modified microfluidic chip

Cited by 18 publications

References 27 publications

Advances in isolation and detection of circulating tumor cells based on microfluidics

Advances in isolation and detection of circulating tumor cells based on microfluidics

Microfluidics for studying metastatic patterns of lung cancer

Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

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