Advances in isolation and detection of circulating tumor cells based on microfluidics

Zou, Dan; Cui, Daxiang

doi:10.20892/j.issn.2095-3941.2018.0256

Cited by 57 publications

(35 citation statements)

References 86 publications

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“…Recently, Strep-tag technique is studied to isolate CTCs with 79% of capture efficiency using EpCAM, HER2, EGFR biomarker and magnetic beads, which showed CTC enrichment under external magnetic field with very fast isolation 32 . This process relies on an expensive EpCAM biomarker, which leads expensive detection and limits its wide-spread use [33][34][35] . Wen et al proposed similar magnetic nanospherical modules, prepared by an emulsifier-free polymerization method coupled with layer-by-layer assembly to capture rare tumor cells 36 .…”

mentioning

confidence: 99%

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Hazra

Kale²,

Aland³

et al. 2020

Sci Rep

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Herein we report a hierarchically organized, water-dispersible ‘nanocage’ composed of cellulose nanocrystals (CNCs), which are magnetically powered by iron oxide (Fe3O4) nanoparticles (NPs) to capture circulating tumor cells (CTCs) in blood for head and neck cancer (HNC) patients. Capturing CTCs from peripheral blood is extremely challenging due to their low abundance and its account is clinically validated in progression-free survival of patients with HNC. Engaging multiple hydroxyl groups along the molecular backbone of CNC, we co-ordinated Fe3O4 NPs onto CNC scaffold, which was further modified by conjugation with a protein - transferrin (Tf) for targeted capture of CTCs. Owing to the presence of Fe3O4 nanoparticles, these nanocages were magnetic in nature, and CTCs could be captured under the influence of a magnetic field. Tf-CNC-based nanocages were evaluated using HNC patients’ blood sample and compared for the CTC capturing efficiency with clinically relevant Oncoviu platform. Conclusively, we observed that CNC-derived nanocages efficiently isolated CTCs from patient’s blood at 85% of cell capture efficiency to that of the standard platform. Capture efficiency was found to vary with the concentration of Tf and Fe3O4 nanoparticles immobilized onto the CNC scaffold. We envision that, Tf-CNC platform has immense connotation in ‘liquid biopsy’ for isolation and enumeration of CTCs for early detection of metastasis in cancer.

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mentioning

confidence: 99%

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Hazra

Kale²,

Aland³

et al. 2020

Sci Rep

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“…However, the information that could come from the investigation of these cells is enormous. Therefore, it is worth the effort to find new methods to isolate and study them [ 78 ]. Characterizing these cells could give information about the stage of a tumor.…”

Section: Resultsmentioning

confidence: 99%

Tumor Hypoxia and Circulating Tumor Cells

Tinganelli

Durante

2020

IJMS

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Circulating tumor cells (CTCs) are a rare tumor cell subpopulation induced and selected by the tumor microenvironment’s extreme conditions. Under hypoxia and starvation, these aggressive and invasive cells are able to invade the lymphatic and circulatory systems. Escaping from the primary tumor, CTCs enter into the bloodstream to form metastatic deposits or re-establish themselves in cancer’s primary site. Although radiotherapy is widely used to cure solid malignancies, it can promote metastasis. Radiation can disrupt the primary tumor vasculature, increasing the dissemination of CTCs. Radiation also induces epithelial–mesenchymal transition (EMT) and eliminates suppressive signaling, causing the proliferation of existent, but previously dormant, disseminated tumor cells (DTCs). In this review, we collect the results and evidence underlying the molecular mechanisms of CTCs and DTCs and the effects of radiation and hypoxia in developing these cells.

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“…Microfluidic methods allow for gentle capturing of live CTCs so that further downstream analysis can be performed using cellular, microscopic, or molecular approaches, to meet the clinical or research need. Microfluidic based CTC-capture devices can be modified to exploit physical and/or biological properties between CTCs and the background cells, thus enabling isolation 24. On the basis of microfluidic technique, CytoSorter® CTC-capture system employs an antibody-immobilized chip for immune capture of CTCs, which are then verified by immunofluorescence staining for PanCK, CD45, and DAPI.…”

Section: Discussionmentioning

confidence: 99%

<p>Evaluation of therapeutic efficacy with CytoSorter<sup>®</sup>, circulating tumor cell–capture system in patients with locally advanced head and neck squamous cell carcinoma</p>

Zheng¹,

Zhang²,

Guo³

et al. 2019

CMAR

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Objective: This study aimed to investigate the feasibility of utilizing CytoSorter® system to detect circulating tumor cells (CTCs) and clinical value of CTCs in patients with locally advanced head and neck squamous cell carcinoma (LAHNSCC). Methods: 31 patients with LAHNSCC, 12 healthy volunteers, and 6 patients with benign tumor serving as controls were enrolled in this study. CTCs were enriched with the CytoSorter®, a microfluidic based immune capture system. CTC detection was performed before and after induction chemotherapy, as well as after surgery and/or radiotherapy. Correlations between CTC enumerations at different time points and survival outcome and recurrence risk were evaluated. The correlation between CTCs and clinicopathological characteristics was appraised. Follow-up of patients continued until March 2019. Results: While CTCs were not found in the controls, they were detected in 24 of 31 LAHNSCC patients. CTCs could be used to distinguish diseased people from the healthy ( P <0.0001). CTCs were statistically associated with patient age ( P =0.037, >60 years old vs<60 years old) and lymph node metastasis ( P = 0.034, N0N1 VS N2N3). Most patients had significantly reduced CTCs at the end of treatment. Patients with partial remission of tumor after induction therapy had more CTCs than those with complete remission of tumor. Patients with higher CTCs counts prior to treatment had higher chance of developing local recurrence of tumor after treatment ( P =0.0187). Conclusion: CTCs were successfully isolated in LAHNSCC patients using CytoSorter® system with better sensibility. CTCs can be used to differentiate LAHNSCC patients from those with benign HNSCC tumor or healthy volunteers, and as markers to monitor patient’s response to treatment and predict the local tumor recurrence after treatment. CTC detection at baseline has the greatest prognostic potency in LAHNSCC patients.

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Advances in isolation and detection of circulating tumor cells based on microfluidics

Cited by 57 publications

References 86 publications

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Tumor Hypoxia and Circulating Tumor Cells

<p>Evaluation of therapeutic efficacy with CytoSorter<sup>®</sup>, circulating tumor cell–capture system in patients with locally advanced head and neck squamous cell carcinoma</p>

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