Epithelial Cell Adhesion Molecule

Trzpis, Monika; McLaughlin, Pamela M.J.; Leij, Lou M.F.H. de; Harmsen, Martin C.

doi:10.2353/ajpath.2007.070152

Cited by 476 publications

(249 citation statements)

References 70 publications

Supporting

Mentioning

224

Contrasting

Unclassified

Order By: Relevance

“…5), indicating that EpCAM does not belong to the category of lineage commitment or cell fate determination genes (62,63). Our findings reflect the fact that EpCAM is virtually reintroduced in mature tissues (64), especially in epithelia cells. Therefore, our results suggest that, to facilitate the dynamic expression pattern of EpCAM during development, the chromatin state of its promoter is elaborately modulated by histone-modifying enzymes, such as SUZ12 and JMJD3, but not DNA methylation (Fig.…”

Section: Discussionmentioning

confidence: 63%

Epithelial Cell Adhesion Molecule Regulation Is Associated with the Maintenance of the Undifferentiated Phenotype of Human Embryonic Stem Cells

Liao

et al. 2010

Journal of Biological Chemistry

112

125

View full text Add to dashboard Cite

Human embryonic stem cells (hESCs) are unique pluripotent cells capable of self-renewal and differentiation into all three germ layers. To date, more cell surface markers capable of reliably identifying hESCs are needed. The epithelial cell adhesion molecule (EpCAM) is a type I transmembrane glycoprotein expressed in several progenitor cell populations and cancers. It has been used to enrich cells with tumor-initiating activity in xenograft transplantation studies. Here, we comprehensively profile the expression of EpCAM by immunofluorescence microscopy, Western blotting, and flow cytometry using an anti-EpCAM monoclonal antibody (mAb) OC98-1. We found EpCAM to be highly and selectively expressed by undifferentiated rather than differentiated hESCs. The protein and transcript level of EpCAM rapidly diminished as soon as hESC had differentiated. This silencing was closely and exclusively associated with the radical transformation of histone modification at the EpCAM promoter. Moreover, we demonstrated that the dynamic pattern of lysine 27 trimethylation of histone 3 was conferred by the interplay of SUZ12 and JMJD3, both of which were involved in maintaining hESC pluripotency. In addition, we used chromatin immunoprecipitation analysis to elucidate the direct regulation by EpCAM of several reprogramming genes, including c-MYC, OCT-4, NANOG, SOX2, and KLF4, to help maintain the undifferentiation of hESCs. Collectively, our results suggest that EpCAM might be used as a surface marker for hESC. The expression of EpCAM may be regulated by epigenetic mechanisms, and it is strongly associated with the maintenance of the undifferentiated state of hESCs.

show abstract

Section: Discussionmentioning

confidence: 63%

Epithelial Cell Adhesion Molecule Regulation Is Associated with the Maintenance of the Undifferentiated Phenotype of Human Embryonic Stem Cells

Liao

et al. 2010

Journal of Biological Chemistry

112

125

View full text Add to dashboard Cite

show abstract

“…In summary, the isolation and characterization of individual tumor cells from the blood of patients known to have metastatic cancer hold tremendous potential for new biological insight with very real clinical applications [45]. Traditionally, CTC's are isolated using the well-characterized surface molecule EpCAM that is expressed on various epithelial tumors [46]. Therefore, the recovery/detection might be improved if a combination with additional tumor cell markers is applied [47,48].…”

Section: Discussionmentioning

confidence: 99%

Phase I Dose Escalation Study with the Lewis Y Carbohydrate Specific Humanized Antibody IGN311

Oruzio¹,

Waxenecker²,

Aulmann³

et al. 2011

JCT

View full text Add to dashboard Cite

show abstract

“…53 In addition, EpCAM is not solely expressed in epithelial cells but likewise strongly expressed in various tissue stem cells, precursors, and in embryonic stem (ES) cells of murine and human origin. 53,54 In ES cells, EpCAM is mandatory for the maintenance of selfrenewal and the pluripotent phenotype. 18,19 Its mode of signaling proceeds via regulated intramembrane proteolysis (see left part of Fig.…”

Section: Do Not Distributementioning

confidence: 99%

EpCAM and its potential role in tumor-initiating cells

Imrich

Hachmeister²,

Gires³

2012

Cell Adhesion & Migration

167

132

View full text Add to dashboard Cite

These authors contributed equally to this work. Keywords: EpCAM, tumor inducing cells, cancer therapyEpithelial cell adhesion molecule EpCAM is expressed on a subset of normal epithelia and overexpressed on malignant cells from a variety of different tumor entities. This overexpression is even more pronounced on so-called tumorinitiating cells (TICs) of many carcinomas. Taking this rather ubiquitous expression of EpCAM in carcinomas and TICs into account, the question arises how EpCAM can serve as a reliable marker for tumor-initiating cells and what might be the advantage for TICs to express this molecule. Furthermore, several approaches for therapeutic strategies targeting exclusively EpCAM on cancer cells were undertaken over the past decades and have recently been transferred to pre-clinical attempts to eradicate TICs. In the present review, we will depict potential functions of EpCAM in tumor cells with a special focus on TICs and therapeutic implications.

show abstract

Epithelial Cell Adhesion Molecule

Cited by 476 publications

References 70 publications

Epithelial Cell Adhesion Molecule Regulation Is Associated with the Maintenance of the Undifferentiated Phenotype of Human Embryonic Stem Cells

Epithelial Cell Adhesion Molecule Regulation Is Associated with the Maintenance of the Undifferentiated Phenotype of Human Embryonic Stem Cells

Phase I Dose Escalation Study with the Lewis Y Carbohydrate Specific Humanized Antibody IGN311

EpCAM and its potential role in tumor-initiating cells

Contact Info

Product

Resources

About