Epithelial Cell Abnormalities: Squamous

Gatscha, Rose Marie; Luff, Ronald D.; Prey, Marianne U.

doi:10.1007/978-1-4612-2042-8_5

Cited by 7 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding is remarkable because changes in nuclear size and morphology are one of the diagnostic criteria for the presence of precancerous lesions and cancer (Crum and Lee, 2006; Wright et al, 2004). …”

Section: Resultsmentioning

confidence: 95%

“…Increased nuclear size, increased DNA content, and tetraploidy are characteristic of Low grade Squamous Intraepithelial Lesions (LSIL) (Mittal, Chan, and Demopoulos, 1990; Prasad et al, 1993). Many of these changes are criteria used in the clinical detection of cervical cancer precursors in screening Pap tests (Crum and Lee, 2006; Wright et al, 2004). Additional characteristics of precancerous lesions have also been reported: the presence of bi-nucleated cells, increased DNA content per cell, and polyploidy (Olaharski et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of HPV16 E5 produces enlarged nuclei and polyploidy through endoreplication

Potapova

et al. 2010

Virology

View full text Add to dashboard Cite

Anogenital cancers and head and neck cancers are causally-associated with infection by high-risk human papillomavirus (HPV). The mechanism by which high-risk HPVs contribute to oncogenesis is poorly understood. HPV16 encodes three genes (HPV16 E5, E6, and E7) that can transform cells when expressed independently. HPV16 E6 and E7 have well-described roles causing genomic instability and unregulated cell cycle progression. The role of HPV16 E5 in cell transformation remains to be elucidated. Expression of HPV16 E5 results in enlarged, polyploid nuclei that are dependent on the level and duration of HPV16 E5 expression. Live-cell imaging data indicate these changes do not arise from cell-cell fusion or failed cytokinesis. The increase in nuclear size is a continual process that requires DNA synthesis. We conclude HPV16 E5 produces polyploid cells by endoreplication. These findings provide insight into how HPV16 E5 can contribute to cell transformation.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Expression of HPV16 E5 produces enlarged nuclei and polyploidy through endoreplication

Potapova

et al. 2010

Virology

View full text Add to dashboard Cite

show abstract

“…Spindle cells were in fact, although these cells may be observed as a result of a reaction surface epithelial in HPV infection, also are associated with severe lesions. Cells of HSIL may be pleomorphic with marked variation of nuclear size and cellular shape, including elongate, spindle, caudate, and apdote cells 28…”

Section: Discussionmentioning

confidence: 99%

Cytopathic effects of human papillomavirus infection and the severity of cervical intraepithelial neoplasia : A frequency study

Sousa

Alves

Martins

et al. 2011

Diagnostic Cytopathology

View full text Add to dashboard Cite

Cytopathic effects related to the human papillomavirus (HPV) infection are more frequently found in cervical intraepithelial neoplasia (CIN) 1; however, there are indications that at least half the histological diagnoses of CIN2 and CIN3 include koilocytosis areas. The objective of this study was to evaluate the frequency of the cytological criteria suggestive of HPV infection in the cervical smears of women with a histological diagnosis of CIN. One hundred and sixty-two women with abnormal cervical smears and a diagnosis of CIN confirmed by histopathology were selected, including 46 cases of CIN 1, 42 of CIN 2 and 74 cases of CIN 3. Koilocytosis was found in 63% of the smears from women with a histopathological diagnosis of CIN 1. This sign was observed in 26.2% and 25.7% of smears of women with a diagnosis of CIN 2 and CIN 3, respectively. Cytomegaly also was frequent in cervical smears of women with histopathological diagnosis of CIN 1 (71.8%). On the other hand, spindle cells and atypical metaplasia were more frequent in women with CIN 2 and CIN 3. Atypical parakeratosis showed similar frequency in all grades of CIN diagnosis. Koilocytois and cytomegaly were inversely correlated with the diagnosis of CIN2 or CIN 3, with OR values respectively of 0.30 (95%CI 0.13-0.68) and 0.26 (95%CI 0.11-0.58). The others signs analyzed did not show any significant association. Koilocitosis and cytomegaly can provides good reassurance that a patient with atypical cervical smear have CIN 1.

show abstract

“…Cytology results were reported according to TBS 2001, for cervicovaginal cytologic diagnosis. 3 Totally, 40 cases that meet the highgrade dysplasia criteria were included in the study. Institutional Review Board approved the study.…”

Section: Methodsmentioning

confidence: 99%

“…2 TBS classifies cytologically detected cervical intraepithelial lesions (CIN) into low-grade (LSIL) and high-grade squamous intraepithelial lesions (HSIL). 3 They acknowledge that most LSIL are self-limited, while HSIL is more likely to persist or progress to invasive cancer, and has led to the effort to improve the sensitivity of Pap smear. 4,5 Therefore, cases of HSIL should be appropriately treated, whereas cases of LSIL may be safely followed under cytologic guidance and colposcopy.…”

mentioning

confidence: 99%

Cytomorphologic analysis and histological correlation of high-grade squamous intraepithelial lesions in postmenopausal women

Kanbour‐Shakir

Modery

et al. 2006

Diagn. Cytopathol.

View full text Add to dashboard Cite

Atrophy-related epithelial changes often pose a diagnostic difficulty during the interpretation of postmenopausal smears. The objectives of this study are to identify the cytomorphologic features of HSIL, in Pap smears of postmenopausal Pap women, and to investigate the possible criteria that could lead to an inaccurate interpretation and false-positive results. Forty Pap smears that were reported as HSIL in postmenopausal women were reviewed. Follow-up cervical biopsies were available on all cases, of which 6 cases were immunostained for MIB-1 and P16. The following cytomorphologic features were evaluated: smear background, degree of cellularity, cellular arrangement, nuclear size, nuclear membrane irregularity, nuclear/cytoplasmic ratio, hyperchromasia, and chromatin pattern. Significant histological abnormalities were present in 35 out of 40 cases. Of those, 22 (55%) cases had high-grade cervical intraepithelial neoplasia (CIN2 or CIN3), 10 (25%) had CIN-1, 5 (12.5%) had reactive changes in the biopsy, and 3 cases had invasive squamous cell carcinoma. The cytomorphologic features that favored HSIL (P < 0.05) included: increased number of abnormal cells, nuclear membrane irregularities, cellular arrangement, and high nuclear/cytoplasmic ratio. Granular background, nuclear size, hyperchromasia, and abnormal chromatin pattern can be associated with reactive and atrophic changes. Our study showed that cytomorphological features favoring HSIL in postmenopausal smears include increased number of abnormal single cells with high nuclear/cytoplasmic ratio and irregular nuclear membrane. Granular background, nuclear enlargement, abnormal chromatin pattern, and hyperchromasia can be seen in reactive changes, and may lead to inaccurate interpretation.

show abstract

Epithelial Cell Abnormalities: Squamous

Cited by 7 publications

References 25 publications

Expression of HPV16 E5 produces enlarged nuclei and polyploidy through endoreplication

Expression of HPV16 E5 produces enlarged nuclei and polyploidy through endoreplication

Cytopathic effects of human papillomavirus infection and the severity of cervical intraepithelial neoplasia : A frequency study

Cytomorphologic analysis and histological correlation of high-grade squamous intraepithelial lesions in postmenopausal women

Contact Info

Product

Resources

About