Epimedium polysaccharide and propolis flavone can synergistically stimulate lymphocyte proliferation in vitro and enhance the immune responses to ND vaccine in chickens

Adjuvanticity of epimedium polysaccharide-propolis flavone on inactivated vaccines against AI and ND virus

“…These indicated that EPA could significantly promote the lymphocyte proliferation, enhance the serum AIV and NDV antibody titers in chickens and possessed better protective effect from infection of ND compared with non-adjuvant. Our previous researches also found that EPA could enhance the immune effect of Newcastle disease attenuated vaccine and rabbit hemorrhagic disease (RHD) vaccine, and reduced the mortality and morbidity of the ND and RHD [12,19].…”

“…Our previous researches demonstrated that epimedium polysaccharide (EP) and propolis flavone (PF) could synergistically enhance cellular and humoral immunity in vitro and in vivo, and possess better immune enhancement and antiviral effect [12,13]. In present research, the inactivated avian influenza (AI) vaccine and Newcastle disease (ND) vaccine containing three doses of epimedium polysaccharide-propolis flavone adjuvant (EPA) were prepared.…”

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

“…The A 570 value and proliferation rate of lymphocyte can reflect the vitality of the lymphocyte and the activity strength of drug in promoting cellular immunity [26,27]. The results of experiment in vitro showed that during single stimulation of polysaccharide, the A 570 values in sOPS 80 , OPS 80 and JPS 50 at 0.9766-15.625 g mL −1 , JPS t and sJPS 50 at 9.766-7.812 g mL −1 , sOPS t , OPS t and sJPS t at 0.9776-3.906 g mL −1 groups were significantly larger than that of the corresponding cell control group respectively; The lymphocyte proliferation rates in four sPSs groups were significantly or numerically higher than that of corresponding uPS group respectively, in sOPS 80 group was the highest and significantly higher than those in all uPS groups.…”

Liposome and epimedium polysaccharide-propolis flavone can synergistically enhance immune effect of vaccine

“…10.008 mRNA expression of IL-2, IL-10 and IFN-␥ of peripheral T lymphocyte in chicken and rabbit [9,10]. The recent researches confirmed that EP and PF possessed synergistically immunologic enhancement and antiviral effect, and the efficacy of EP-PF (named EP-PF immunopotentiator, EPI)was better than those of epimedium flavone plus PF, epimedium flavone plus propolis extracts, and EP plus propolis extracts [11,12]. Therefore, EPI had been applied for national new veterinary drug and invent patent in China and will be expected to exploit into a new immunopotentiator.…”