Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo

Abstract

Stress-induced fibroblast senescence is thought to contribute to skin aging. Ultraviolet light (UV) radiation is the most potent environmental risk factor in these processes. An Epilobium angustifolium (EA) extract was evaluated for its capacity to reverse the senescent response of normal human dermal fibroblasts (NHDF) in vitro and to exhibit skin photo-protection in vivo. The HPLC-UV-MS analysis of the EA preparation identified three major polyphenol groups: tannins (oenothein B), phenolic acids (gallic and chlorogenic acids) and flavonoids. EA extract increased the cell viability of senescent NHDF induced by serum deprivation. It diminished connective tissue growth factor and fibronectin gene expressions in senescent NHDF. Down-regulation of the UV-induced release of both matrix metalloproteinase-1 and -3 and the tissue inhibitor of matrix metalloproteinases-1 and -2, and also down-regulation of the gene expression of hyaluronidase 2 were observed in repeatedly UV-irradiated NHDF after EA extract treatment. Interestingly, EA extract diminished the down-regulation of sirtuin 1 dampened by UV-irradiation. The application of EA extract using a sub-irritating dose protected skin against UV-induced erythema formation in vivo. In summary, EA extract diminished stress-induced effects on NHDF, particularly on connective tissue growth factor, fibronectin and matrix metalloproteinases. These results collectively suggest that EA extract may possess anti-aging properties and that the EA polyphenols might account for these benefits.

Modulation of keratin 1, 10 and involucrin expression as part of the complex response of the human keratinocyte cell line HaCaT to ultraviolet radiation

“…The spontaneously immortalized human keratinocyte cell line HaCaT (gift of Prof. Dr. N. Fusenig (Boukamp et al , 1988)) was grown in Dulbecco's modified Eagle's medium (DMEM, Sigma, St Louis, Missouri) supplemented with 10% fetal bovine serum (BFS, Invitrogen, Carlsbad, CA, USA), glutamine (0.29 mg/ml) (Sigma) and gentamycin (50 µg/ml) (Invitrogen) in 5% CO 2 at 37 °C, as described previously (Ruszova et al , 2013; Ruszova et al , 2008). …”

Low molecular weight hyaluronan mediated CD44 dependent induction of IL-6 and chemokines in human dermal fibroblasts potentiates innate immune response

“…NHDF were obtained from the dermis by the digestion method [20]. Briefly, tissue specimens ($3 cm 2 ) were rinsed with sterile phosphate buffer saline (PBS) and cut into smaller pieces ($0.5 cm 2 ), and the subcutaneous fat and epidermis were removed.…”

An overview about oxidation in clinical practice of skin aging

“…Ruszova et al (2013) 73 showed, by in vitro assays, beneficial effect of Epilobium angustifolium extract, after exposure, through downregulation of expression of MMP-1 genes and MMP-3 (collagenases) and hyaluronidase-2. Considering keratinocytes cell lines it is possible to recognize the properties of antioxidants in reducing the formation of free radicals after UVB radiation or induced by hydrogen peroxide in cell cultures (HaCat).…”

“…Ruszova et al. (2013) 73 have shown photoprotective action, in vivo , employing formulation provided of E. angustifolium extract.…”