Epigenetic role of epidermal growth factor expression and signalling in embryonic mouse lung morphogenesis

Warburton, David; Seth, Rajeev; Shum, Lillian; Horcher, Philip G.; Hall, Frederick L.; Werb, Zena; Slavkin, Harold C.

doi:10.1016/0012-1606(92)90269-m

Cited by 180 publications

(98 citation statements)

References 30 publications

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“…16 Both FGF7 and HGF appear to favor growth stimulation of epithelial cells from human pancreatic ducts 2,6 and EGF signaling is important for development of organs, like the pancreas, requiring epithelial-mesenchymal interaction. 17 The effect of EGF on proliferation and motility of progenitor(-like) cells, other than pancreatic, has been extensively described. [18][19][20][21] Moreover, EGF receptors have been detected in adult human and in developing mouse pancreatic ducts [22][23][24] and are critical for proper pancreas development.…”

Section: Discussionmentioning

confidence: 99%

EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade

Rescan

Bras

Lefebvre

et al. 2005

Laboratory Investigation

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Human postnatal pancreatic duct cells are a potential source of new beta cells. Factors regulating proliferation of human pancreatic duct cells in vitro are unknown. In several other cell types, this process is influenced by ligands of the ErbB receptor family. The expression and functionality of the ErbB family members and their possible role in duct cell proliferation were determined. In cultured adult human pancreatic duct cells the different members of the ErbB family (ErbB1-4) were present at transcript and protein level. Stimulation of the duct cells with epidermal growth factor (EGF) and betacellulin results in Tyr-phosphorylation of ErbB1 and ErbB2, followed by activation of Shc, MEK1/2 and ERK1/2. Duct cells with activated ErbB signaling changed morphology and motility. EGF induced proliferation of a fraction of the duct cells and treatment with PD98059 prevented Ki67 expression in EGF-supplemented cells. When transduced with recombinant adenovirus expressing constitutively activated MEK1, duct cells proliferate and spread even in the absence of EGF. Importantly, the adult human duct cells retain their capacity to recapitulate ngn3-induced embryonic (neuro)endocrine differentiation after proliferation. Therefore, the present data support a possible role for human adult pancreatic duct cells, following expansion and transdifferentiation, as a source of insulin by transplantation to type I diabetes patients.

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Section: Discussionmentioning

confidence: 99%

EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade

Rescan

Bras

Lefebvre

et al. 2005

Laboratory Investigation

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“…TGF-a transcripts have been detected in the unfertilized mouse oocyte and are rapidly destroyed at the two-cell stage, but are later resynthesized by the preimplantation conceptus (1,17). In contrast, expression of the EGF gene is not observed until after implantation and the beginning of organogenesis (18)(19)(20). EGFR mRNA and protein have been identified throughout the development of the mouse embryo from the unfertilized oocyte (21) to the postimplantation embryo (22,23).…”

Section: Discussionmentioning

confidence: 99%

Expression of transforming growth factor alpha (TGF-α) gene in mouse embryonic development

Harada

Fujikawa

Yoshida

et al. 1997

J Assist Reprod Genet

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“…By their nature, protein tyrosine phosphatases such as LAR and PTPG are likely to function in conjunction with tyrosine kinases to fine tune the regulation of tyrosine phosphorylation and therefore to control cell growth and differentiation. Many receptor tyrosine kinases have been identified in both epithelial and mensenchymal cells of the lung and their expression patterns overlap with LAR or PTPG in the lung at various developmental stages (Buch et al, 1991;Warburton et al, 1992;Peters et al, 1992), suggesting functional interactions between the two classes of receptors. Since the expression of LAR and PTPG is relatively constant during lung development in comparison to the receptor tyrosine kinases, each may be involved in more than one biological event with relatively non-specific interactions with tyrosine kinases.…”

Section: Discussionmentioning

confidence: 99%

Two closely related receptor‐type tyrosine phosphatases are differentially expressed during rat lung development

Katsura

Williams

Brody

et al. 1995

Developmental Dynamics

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Transmembrane protein tyrosine phosphatases (PTPases) comprise a newly identified class of receptor-like molecules. In most cases their ligands and the substrates they dephosphorylate are not known. In order to begin to explore the functions of the PTPases in cell physiology and in mammalian development, we examined the expression patterns of two closely related receptor-type tyrosine phosphatase genes, namely LAR and PTPG, in fetal rat lung and in selected adult rat tissues. In the lung, in situ hybridization and immunohistochemistry show that the LAR mRNA and protein are expressed exclusively in the epithelium. In the early embryonic or fetal lung (day 13 to 18) LAR is expressed by all of the epithelial cells of the forming bronchial tree. This widespread pattern of expression is lost later in fetal Life (day 21) as the lung matures and acquires the morphologic and biochemical features of the adult organ. LAR gene expression is then confined to two epithelial progenitor cells of the distal airways, namely the bronchiolar Clara cell and the alveolar type I1 cell. The LAR gene products were also found abundantly expressed in epithelial progenitor cells of adult esophagus, skin, and small intestine, all of which are continuously renewing epithelia. The rat PTPG gene, on the other hand, is specifically expressed in the mesenchyme of the developing lung. The level of the PTPG mRNA decreases as the lung matures. These results suggest that the two closely related receptor-type tyrosine phosphatases are differentially expressed in a tissue-specific fashion. They are expressed mostly in proliferating cells or in cells which have potential to proliferate. Therefore, one function of the two receptor-like tyrosine phosphatases may be to regulate proliferation and/or differentiation of different types of cells during development, during normal cell turnover, and during adult tissue repair.

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Epigenetic role of epidermal growth factor expression and signalling in embryonic mouse lung morphogenesis

Cited by 180 publications

References 30 publications

EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade

EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade

Expression of transforming growth factor alpha (TGF-α) gene in mouse embryonic development

Two closely related receptor‐type tyrosine phosphatases are differentially expressed during rat lung development

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