Expression of transforming growth factor alpha (TGF-α) gene in mouse embryonic development

Harada, Tasuku; Fujikawa, Tomoyuki; Yoshida, Souichi; Onohara, Yoshimasa; Tanikawa, Masahiro; Terakawa, Naoki

doi:10.1007/bf02765827

Cited by 14 publications

(12 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The EGF ligand is involved in the formation of hatched blastocysts [12], but its receptor remains at low levels until the four-cell stage [13]. Although TGF␣ reduces the incidence of apoptosis in mouse blastocysts [14], it is not detected before the four-cell stage [15]. Thus, receptor PTKs for peptide growth factors do not seem to be involved in the regulation of development before the four-cell stage.…”

Section: Introductionmentioning

confidence: 94%

Localization of Janus Kinase 2 to the Nuclei of Mature Oocytes and Early Cleavage Stage Mouse Embryos

Ito

Nakasato

Suzuki

et al. 2004

Biology of Reproduction

View full text Add to dashboard Cite

Jak2, which is a member of the Janus tyrosine kinase family, plays essential roles in cytokine signal transduction and in the regulation of cell growth and gene expression. To investigate the involvement of Jak2 in the regulation of early preimplantation development, we examined the expression of Jak2 in mouse embryos. Reverse transcription-polymerase chain reaction assays revealed that the relative amount of Jak2 mRNA was highest in unfertilized oocytes, gradually decreased until the four-cell stage, and remained at low levels until the blastocyst stage. Immunocytochemistry showed that Jak2 was localized predominantly to the female pronucleus in one-cell embryos. The immunofluorescence signal was very weak or undetectable in the male pronucleus. In unfertilized oocytes and one-cell embryos at M phase, Jak2 was localized to the chromosomes. After cleavage to the two-cell stage, the intensity of the immunofluorescence signal decreased in the nucleus while the embryos were in late G2. This decrease was independent of DNA synthesis because it was not affected by inhibition of DNA replication. However, inhibition of protein synthesis repressed the disappearance of Jak2 from the nucleus. These results suggest a novel function for Jak2 in the regulation of early preimplantation development.

show abstract

Section: Introductionmentioning

confidence: 94%

Localization of Janus Kinase 2 to the Nuclei of Mature Oocytes and Early Cleavage Stage Mouse Embryos

Ito

Nakasato

Suzuki

et al. 2004

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…TGF-α has been demonstrated to stimulate DNA and protein synthesis in blastocysts as well asincrease the rate of blastocoel expansion. Administration of TGF-α antisense ODN significantly reduces the rate of blastocoel expansion 28. Rate of blastocoel expansion is shown to increase in the presence of acrogranin.…”

Section: Discussionmentioning

confidence: 92%

Contraceptive Vaccines Targeting Factors Involved in Establishment of Pregnancy

Lemons

Naz

2011

American J Rep Immunol

View full text Add to dashboard Cite

Citation Lemons AR, Naz RK. Contraceptive vaccines targeting factors involved in establishment of pregnancy. Am J Reprod Immunol 2011; 66: 13–25 Current methods of contraception lack specificity and are accompanied with serious side effects. A more specific method of contraception is needed. Contraceptive vaccines can provide most, if not all, the desired characteristics of an ideal contraceptive. This article reviews several factors involved in the establishment of pregnancy, focusing on those that are essential for successful implantation. Factors that are both essential and pregnancy‐specific can provide potential targets for contraception. Using database search, 76 factors (cytokines/chemokines/growth factors/others) were identified that are involved in various steps of the establishment of pregnancy. Among these factors, three, namely chorionic gonadotropin (CG), leukemia inhibitory factor (LIF), and pre‐implantation factor (PIF), are found to be unique and exciting molecules. Human CG is a well‐known pregnancy‐specific protein that has undergone phase I and phase II clinical trials, in women, as a contraceptive vaccine with encouraging results. LIF and PIF are pregnancy‐specific and essential for successful implantation. These molecules are intriguing and may provide viable targets for immunocontraception. A multiepitope vaccine combining factors/antigens involved in various steps of the fertilization cascade and pregnancy establishment may provide a highly immunogenic and efficacious modality for contraception in humans.

show abstract

“…Previous studies have revealed transcripts for this growth factor in embryos of several species, including human (Klonisch et al, 2001), bovine (Chia et al, 1995;Kobayashi et al, 1994), porcine (Singh et al, 1993) and mouse (Das et al, 1991;Harada et al, 1997). EGF mRNA is rapidly down-regulated in mouse embryos (Das et al, 1991;Moller et al, 2001), suggesting that although detection in zygotes is due to internalization of maternal EGF during oogenesis, IVF-derived and cloned embryos are capable of expressing EGF independent of maternal influence (Kidder, 1992;Wiley et al, 1992).…”

Section: Figmentioning

confidence: 97%

Expression Levels of EGF, TGF-α, and EGF-R Are Significantly Reduced in Pre-Implantation Cloned Mouse Embryos

Dadi

Lloyd

2004

Cloning and Stem Cells

View full text Add to dashboard Cite

Altered expression of genes encoding mitogenic growth factors thought to play key regulatory roles in preimplantation development may contribute to poor cloning efficiency. In this study, we compared the pattern, timing, and level of expression of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-␣) genes and their cognate receptor EGF-R in mouse embryos derived by cloning with in vivo-(naturally fertilized) and in vitro-fertilized embryos at four preimplantation stages of development. In cloned embryos, EGF, TGF-␣, and EGF-R expression levels were nearly undetectable, while expression of a housekeeping gene, transcription factor SP1, was normal. In contrast, growth factor genes were expressed in in vitro-fertilized embryos, although significantly less than in in vivo embryos (p Ͻ 0.05). In all embryos examined, the timing and pattern of gene expression were similar. Expression in the gravid uterus paralleled that in preimplantation embryos, while no expression was measured in non-gravid uteri. Our results lead us to speculate that altered levels of expression of EGF, TGF-␣, and EGF-R impair mammalian development, which could account for the poor survival potential of cloned mouse embryos. 267

show abstract

Expression of transforming growth factor alpha (TGF-α) gene in mouse embryonic development

Abstract: Purpose: The expression of genest for TGF-ct, epidermal growth factor (EGF), and the EGF receptor (EGFR) in mouse blastocysts was evaluated by the reverse transcription-polymerase chain reaction (RT-PCR

Cited by 14 publications

References 44 publications

Localization of Janus Kinase 2 to the Nuclei of Mature Oocytes and Early Cleavage Stage Mouse Embryos

Localization of Janus Kinase 2 to the Nuclei of Mature Oocytes and Early Cleavage Stage Mouse Embryos

Contraceptive Vaccines Targeting Factors Involved in Establishment of Pregnancy

Expression Levels of EGF, TGF-α, and EGF-R Are Significantly Reduced in Pre-Implantation Cloned Mouse Embryos

Contact Info

Product

Resources

About