Epigenetic response to nanopolystyrene in germline of nematode Caenorhabditis elegans

Induction of Protective Response Associated with Expressional Alterations in Neuronal G Protein-Coupled Receptors in Polystyrene Nanoparticle Exposed Caenorhabditis elegans

“…To prepare constructs of P unc-14-dcar-1 , P unc-14-daf-37 , or P unc-14 - npr-1 , the cDNA fragment of dcar-1/C06H5.7.1 , daf-37/C30B5.5.1 , or npr-1/C39E6.6.1 was inserted into the pPD95_77 with P unc-14 promoter. In the gonad, the germline transgene was performed by injecting both marker construct (P dop-1::rfp , 45 μg/mL) and testing construct (45 μg/mL) . The primers used for generation of constructs are shown in Table S3.…”

“…In the gonad, the germline transgene was performed by injecting both marker construct (Pdop-1::rfp, 45 μg/mL) and testing construct (45 μg/mL). 59 The primers used for generation of constructs are shown in Table S3. Data Analysis.…”

Response of G protein-coupled receptor CED-1 in germline to polystyrene nanoparticles inCaenorhabditis elegans

“… 32 To obtain a synchronized L1-larvae population, gravid worms were treated with a bleaching solution containing NaOH (0.45 M) and 2% HOCl (2%) to release enough eggs on NGM plates. 33 Following that, the eggs were allowed to develop into L1 larvae.…”

Nanoplastics: Status and Knowledge Gaps in the Finalization of Environmental Risk Assessments

“…Amongst the studied organisms, the nematode Caenorhabditis elegans has emerged as a reliable toxicity model owing to its several practical and technical advantages [ 76 ]. We found 26 articles [ 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 ] reporting the sublethal effects of environmentally relevant concentrations (µg·L −1 ) of NPs on Caenorhabditis elegans . Of these, twenty-three studies examined the effects of nanoPS alone, whilst the other three considered the enhancement of the toxicity of other stressors in combination with exposure to nanoPS.…”