Epigenetic regulation of bone remodeling by natural compounds

Raut, Nishikant A.; Wicks, Sheila M.; Lawal, Tempitope O.; Mahady, Gail B.

doi:10.1016/j.phrs.2019.104350

Cited by 47 publications

(45 citation statements)

References 139 publications

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“…Recent evidence indicates that, among the mechanisms by which naturally occurring compounds, such as resveratrol and anthocyanin, impact bone cell activities, the bone epigenome exerts a fundamental role [19]. The epigenetic regulation of osteoblast activity refers to the mechanisms that impact gene expressions such as post-translational histone modifications, miRNA-mediated post-transcriptional regulation, and DNA methylation [41,42].…”

Section: Discussionmentioning

confidence: 99%

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Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration

Casati

Pagani

Maggi

et al. 2020

IJMS

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Bone remodeling and repair require osteogenic cells to reach the sites that need to be rebuilt, indicating that stimulation of osteoblast migration could be a promising osteoanabolic strategy. We showed that purified δ-tocotrienol (δ-TT, 10 μg/mL), isolated from commercial palm oil (Elaeis guineensis) fraction, stimulates the migration of both MC3T3-E1 osteoblast-like cells and primary human bone marrow mesenchymal stem cells (BMSC) as detected by wound healing assay or Boyden chamber assay respectively. The ability of δ-TT to promote MC3T3-E1 cells migration is dependent on Akt phosphorylation detected by Western blotting and involves Wnt/β-catenin signalling pathway activation. In fact, δ-TT increased β-catenin transcriptional activity, measured using a Nano luciferase assay and pretreatment with procaine (2 µM), an inhibitor of the Wnt/β-catenin signalling pathway, reducing the wound healing activity of δ-TT on MC3T3-E1 cells. Moreover, δ-TT treatment increased the expression of β-catenin specific target genes, such as Osteocalcin and Bone Morphogenetic Protein-2, involved in osteoblast differentiation and migration, and increased alkaline phosphatase and collagen content, osteoblast differentiation markers. The ability of δ-TT to enhance the recruitment of BMSC, and to promote MC3T3-E1 differentiation and migratory behavior, indicates that δ-TT could be considered a promising natural anabolic compound.

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Section: Discussionmentioning

confidence: 99%

“…Finally, we examined the effect of δ-TT on the level of global acetylation of histone H4 and H3, an important epigenetic mark of gene activation, since rrecent evidence indicates that the bone epigenome exerts a fundamental role in the control of bone cell activities [19].…”

Section: Of 17mentioning

confidence: 99%

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration

Casati

Pagani

Maggi

et al. 2020

IJMS

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“…Natural products and derived molecules are indispensable in developing novel treatments for osteoporosis (Raut et al, 2019). Betulinic acid (BA) represents a natural pentacyclic triterpenoid (chemical formula shown in Figure 1), which widely exists in Betula pubescens and a variety of plants.…”

Section: Introductionmentioning

confidence: 99%

Betulinic Acid Protects From Bone Loss in Ovariectomized Mice and Suppresses RANKL-Associated Osteoclastogenesis by Inhibiting the MAPK and NFATc1 Pathways

Wei

Liu

et al. 2020

Front. Pharmacol.

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Osteoclasts with elevated bone resorption are commonly present in postmenopausal osteoporosis, and other osteolytic pathologies. Therefore, suppressing osteoclast generation and function has been the main focus of osteoporosis treatment. Betulinic acid (BA) represents a triterpenoid mainly purified from the bark of Betulaceae. BA shows multiple biological activities, including antitumor and anti-HIV properties, but its effect on osteolytic conditions is unknown. Here, BA suppressed receptor activator of nuclear factor-kB ligand (RANKL)-associated osteoclastogenesis and bone resorptive function, as assessed by tartrate-resistant acid phosphatase (TRAP) staining, fibrous actin ring generation, and hydroxyapatite resorption assays. Mechanistically, BA downregulated the expression of osteoclastic-specific genes. Western blot analysis revealed that BA significantly interrupted ERK, JNK and p38 MAPK activation as well as intracellular reactive oxygen species (ROS) production, thus altering c-Fos and NFATc1 activation. Corroborating the above findings in cell-based assays, BA prevented ovariectomyassociated bone loss in an animal model. In conclusion, these findings suggest that BA can inhibit osteoclast generation and function as well as the RANKL signaling pathway, and might be used for treating osteoclast-related osteoporosis.

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“…Based on literature, active components present in chia seed, may have possible influences toward some of the supposed underlying mechanisms of this disease pathogenesis, such as increasing intestinal binding of calcium and decreasing bone resorption ( 24 , 29 , 30 ). For this reason, chia seed supplementation has been tested in three cats diagnosed with idiopathic hypercalcemia, after dietary change alone did not lead to normocalcemia.…”

Section: Introductionmentioning

confidence: 99%

Managing Feline Idiopathic Hypercalcemia With Chia Seeds (Salvia hispanica L.): A Case Series

Fantinati

Priymenko

2020

Front. Vet. Sci.

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Background: We describe for the first time the use of chia seeds (Salvia hispanica L.) as a non-pharmacological solution in managing feline idiopathic hypercalcemia when dietary change alone fails. Case Summary: Over a 2-year period of time, three female spayed, middle-aged, Domestic Shorthair cats were diagnosed with idiopathic hypercalcemia. Reason for consultation were lethargy and dysorexia, with a single episode of vomiting described in one cat and dysuria in another. Thorough diagnostic work-up included complete blood count, serum biochemistry, urinalysis, ionized calcium, calcemic hormones, parathyroid hormone-related protein, and imaging of chest and abdomen. Based on different nutritional reasons, each cat was switched to a different high-moisture pet food as first-step in managing the disorder: a high-fiber diet, a diet formulated for chronic kidney disease management and a diet designed to prevent calcium oxalate urolithiasis. In the three cats, 6 weeks of dietary change alone did not result in normocalcemia. Before resorting to any pharmacological solution, supplementation to the diet of chia seeds (2 g/cat/day) was started. After 4 weeks from the introduction of Salvia hispanica L., all cats achieved normalization of ionized calcium concentration. Conclusion: Chia seeds (Salvia hispanica L.) supplementation could be a useful tool in managing feline idiopathic hypercalcemia.

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Epigenetic regulation of bone remodeling by natural compounds

Cited by 47 publications

References 139 publications

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration

Betulinic Acid Protects From Bone Loss in Ovariectomized Mice and Suppresses RANKL-Associated Osteoclastogenesis by Inhibiting the MAPK and NFATc1 Pathways

Managing Feline Idiopathic Hypercalcemia With Chia Seeds (Salvia hispanica L.): A Case Series

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