Epigenetic Dynamics of HOXA10 Gene in Infertile Women With Endometriosis

Samadieh, Yasaman; Favaedi, Raha; Ramezanali, Fariba; Afsharian, Parvaneh; Aflatoonian, Reza; Shahhoseini, Maryam

doi:10.1177/1933719118766255

Cited by 27 publications

(18 citation statements)

References 43 publications

(85 reference statements)

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“…In the bivalent state, H3K27me3 is dominant over the H3K4me3 mark, with genes controlled by these marks being repressed as default [7]. The removal of H3K27me3 by histone demethylases then allows the prompt expression of patterning and lineage specifier HOX genes following a temporal and special collinearity [8,9]. At gastrulation, trimethylation of H3K27 mediated by the Polycomb Repressive Complex 2 (PRC2) and recruitment of the Polycomb Repressive Complex 1 (PRC1) induce chromatin condensation to maintain a permanent silencing of the HOX genes in a lineage-specific manner [9,10].…”

Section: Hox Genes Are Master Regulators Of Embryonic Developmentmentioning

confidence: 99%

A Case of Identity: HOX Genes in Normal and Cancer Stem Cells

Smith

Zyoud

Allegrucci

2019

Cancers

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Stem cells are undifferentiated cells that have the unique ability to self-renew and differentiate into many different cell types. Their function is controlled by core gene networks whose misregulation can result in aberrant stem cell function and defects of regeneration or neoplasia. HOX genes are master regulators of cell identity and cell fate during embryonic development. They play a crucial role in embryonic stem cell differentiation into specific lineages and their expression is maintained in adult stem cells along differentiation hierarchies. Aberrant HOX gene expression is found in several cancers where they can function as either oncogenes by sustaining cell proliferation or tumor-suppressor genes by controlling cell differentiation. Emerging evidence shows that abnormal expression of HOX genes is involved in the transformation of adult stem cells into cancer stem cells. Cancer stem cells have been identified in most malignancies and proved to be responsible for cancer initiation, recurrence, and metastasis. In this review, we consider the role of HOX genes in normal and cancer stem cells and discuss how the modulation of HOX gene function could lead to the development of novel therapeutic strategies that target cancer stem cells to halt tumor initiation, progression, and resistance to treatment.

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Section: Hox Genes Are Master Regulators Of Embryonic Developmentmentioning

confidence: 99%

A Case of Identity: HOX Genes in Normal and Cancer Stem Cells

Smith

Zyoud

Allegrucci

2019

Cancers

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“…Ectopic transcriptome studies showed that processes such as cell adhesion, inflammation, immune system regulation, cell migration, regeneration of the extracellular matrix, and angiogenesis were associated with endometriosis (5-7). The expression of HOXA10 gene in the normal endometrium of healthy women during the reproductive cycle has been observed in the proliferative phase and has a significantly increased expression in the luteal phase (8). In women with endometriosis, the expression of the HOXA10 gene decreases dramatically, indicating a defect in uterine acceptance that may be responsible for reduced fertility in women with endometriosis (9).…”

Section: Introductionmentioning

confidence: 99%

The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium

Mirabutalebi

Karami

Montazeri

et al. 2018

IJRM

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Background:The study of microRNA expression can be effective in the diagnosing and treating different diseases. miR-135a is one of the most important micro-ribonucleic acids involved in endometriosis. Among the genes that become the target of the miR-135a and are subjected to changes in the endometrium of patients with endometriosis is HOXA10 gene which is expressed in the endometrium in response to steroid hormones.Objective: The aim of this study was to evaluate the expression of miR-135a and its relationship with the level of HOXA10 gene expression in both endometrial ectopic and eutopic tissues in patients with endometriosis compared to the control samples.Materials and Methods: In this prospective case-control study, both case-eutopic and case-ectopic tissue samples were obtained from 17 women with endometriosis and the eutopic endometrial tissue was sampled from 17 women with normal endometrium as the control group. The gene's expression of miR-135a and HOXA10 were investigated using quantitative reverse transcription PCR (q-RT PCR).Results: A significant decrease in the expression of HOXA10 gene was detected in case-eutopic during the luteal phase compared to the control samples (p=0.001), while in the case-ectopic, the expression of this gene was increased (p=0.681) compared to the control samples. In addition, the expression miR-135a in the luteal phase showed a remarkable increase in the case-eutopic endometrial tissue (p=0.026) as well as a significant decrease in the case-ectopic endometrial tissue compared to the control samples (p=0.008).Conclusion:Considering the inverse relations between the over-expression of miR-135a and the reduction of HOXA10, it seems that miR-135a may be applied as an endometrial diagnostic and therapeutic biomarker.

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“…The pathogenesis of endometriosis which was related to infertility remains unclear, but studies have shown that dysregulation HOX genes are important for the development of endometriosis [2,[26][27][28].…”

Section: Discussionmentioning

confidence: 99%

Network-based bioinformatics analysis of HOX genes and their related genes in endometriosis

Chitsazian

Favaedi

Jahromi

et al. 2020

Preprint

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Background: Dysregulation of some HOX genes are important for the development of endometriosis. Endometriosis is a complex gynecologic disorder affecting as many as 10-15% of premenopausal women. The pathogenesis of endometriosis, as the presence of endometrium-like tissue outside the uterine cavity, is largely unknown. HOX genes, encoding homeodomain transcription factors, are dynamically expressed in endometrium, in which they are necessary for endometrial growth, differentiation, and implantation. Few investigations have been studied in this vast range of HOX genes with a network-based view. Network theory allows for a holistic understanding of the role of genes in diseases. The purpose of this study is to identify genes that cause this disease by focusing on the HOX family genes and their regulatory genes. Materials & Methods: Fifteen samples of eutopic and ectopic endometrium collected from patients in proliferation phase. PCR array was performed on sample groups for 84 HOX genes and their related genes. The statistical significance was determined by using t-test. PCA and Hierarchical clustering were carried out between different groups. The genes co-expression networks were constructed based on correlation between normalized gene expression data. Gene function was annotated based on Gene Ontology, the intervention in other diseases and expression in other tissues using the DAVID to clarify the function of important genes and the mechanism of endometriosis. Results: Among the analyzed genes, 30, 40 and 35 significantly differentially expressed genes were in eutopic, ectopic compared with normal and ectopic with eutopic samples, respectively. Twenty-eight genes had no significant alteration in none of the sample groups. Conclusion: The most significant genes and the genes in co-expression networks are effective genes in development, metabolic and neural processes. Another important point is that the some non-differently expressed genes in networks have many interactions with the significant genes, indicating that these non-significant genes also are likely contributed in endometriosis. Keywords: endometriosis, HOX, PCR-array, Co-expression network

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Epigenetic Dynamics of HOXA10 Gene in Infertile Women With Endometriosis

Cited by 27 publications

References 43 publications

A Case of Identity: HOX Genes in Normal and Cancer Stem Cells

A Case of Identity: HOX Genes in Normal and Cancer Stem Cells

The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium

Network-based bioinformatics analysis of HOX genes and their related genes in endometriosis

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