The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium

Mirabutalebi, Seyed Hamidreza; Karami, Noorodin; Montazeri, Fateme; Fesahat, Farzaneh; Sheikhha, Mohammad Hasan; Hajimaqsoodi, Elnaz; Zarchi, Mojgan Karimi; Kalantar, Seyed Mehdi

doi:10.29252/ijrm.16.8.501

Cited by 12 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the biggest study, which included 50 controls and 32 eutopic samples, elevated levels of hsa-mir-135a in the eutopic endometrium of endometriosis patients were detected, compared to controls [ 6 ]. This was confirmed by a subsequent study, which also found that hsa-mir-135a was upregulated in eutopic endometrium [ 46 ]. While in the first study, the hsa-mir-135a expression was reported to be increased in the proliferative phase [ 6 ], in the second study, expression was upregulated in the secretory phase, although the proliferative phase showed a similar trend [ 46 ].…”

Section: Discussionsupporting

confidence: 63%

“…This was confirmed by a subsequent study, which also found that hsa-mir-135a was upregulated in eutopic endometrium [ 46 ]. While in the first study, the hsa-mir-135a expression was reported to be increased in the proliferative phase [ 6 ], in the second study, expression was upregulated in the secretory phase, although the proliferative phase showed a similar trend [ 46 ]. An independent study focusing solely on endometriosis patients found that hsa-mir-135a expression was significantly reduced in ectopic compared to eutopic endometrium [ 47 ].…”

Section: Discussionsupporting

confidence: 63%

See 1 more Smart Citation

Hsa-mir-135a Shows Potential as A Putative Diagnostic Biomarker in Saliva and Plasma for Endometriosis

Perricos¹,

Proestling²,

Husslein³

et al. 2022

Biomolecules

View full text Add to dashboard Cite

Endometriosis is a chronic disease characterized by the implantation and proliferation of endometrial tissue outside of the uterine cavity. The nonspecific nature of the symptoms and the lack of sensitive, noninvasive diagnostic methods often lead to a significant delay in diagnosis, highlighting the need for diagnostic biomarkers. The correlation of circulating miRNAs with altered inflammatory signals seen in patients with endometriosis has raised the possibility that miRNAs can serve as biomarkers for the disease. In our study, we analyzed miRNA expression in saliva of women with and without endometriosis using a FireFly custom multiplex circulating miRNA assay. This focused panel included 28 human miRNAs, 25 of which have been previously found to be differentially expressed either in plasma, serum, and/or blood of women with endometriosis, compared to controls. We found that hsa-mir-135a was expressed significantly higher in the saliva of women with endometriosis, independent of disease stage and menstrual cycle phase. We confirmed that hsa-mir-135a also showed significantly elevated expression in the plasma of endometriosis patients. This indicates that hsa-mir-135a is a putative noninvasive biomarker of endometriosis in both saliva and plasma, but further validation studies are required to assess its clinical value as a biomarker.

show abstract

Section: Discussionsupporting

confidence: 63%

Section: Discussionsupporting

confidence: 63%

Hsa-mir-135a Shows Potential as A Putative Diagnostic Biomarker in Saliva and Plasma for Endometriosis

Perricos¹,

Proestling²,

Husslein³

et al. 2022

Biomolecules

View full text Add to dashboard Cite

show abstract

“…The impact of miR-135 on endometrial cancer was contradictory in the literature. Wang J. et al (2020) revealed that miR-135a promoted proliferation, migration, and invasion and induced chemoresistance of endometrial cancer cells, but ( Mirabutalebi et al, 2018 ) found miR-135a acted as a tumor suppressor by targeting ASPH in endometrial cancer ( Chen et al, 2019 ; Wang J. et al, 2020 ). A positive correlation was also observed between the expression of miR-135a and endometriosis lesions, which is a disease also referring migration of the endometrium ( Mirabutalebi et al, 2018 ; Petracco et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“… Wang J. et al (2020) revealed that miR-135a promoted proliferation, migration, and invasion and induced chemoresistance of endometrial cancer cells, but ( Mirabutalebi et al, 2018 ) found miR-135a acted as a tumor suppressor by targeting ASPH in endometrial cancer ( Chen et al, 2019 ; Wang J. et al, 2020 ). A positive correlation was also observed between the expression of miR-135a and endometriosis lesions, which is a disease also referring migration of the endometrium ( Mirabutalebi et al, 2018 ; Petracco et al, 2019 ). The expression of miR-449b was markedly reduced in type II endometrial cancer tissues, and its reduction was associated with endometriosis lesions via endometrial stromal cell proliferation and angiogenesis ( Braza-Boils et al, 2014 ; Ye et al, 2014 ; Liu et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of a MicroRNA Signature Associated With Lymph Node Metastasis in Endometrial Endometrioid Cancer

Song

et al. 2021

Front. Genet.

View full text Add to dashboard Cite

BackgroundLymph node metastasis (LNM) is an important prognostic factor in endometrial cancer. Anomalous microRNAs (miRNAs) are associated with cell functions and are becoming a powerful tool to characterize malignant transformation and metastasis. The aim of this study was to construct a miRNA signature to predict LNM in endometrial endometrioid carcinoma (EEC).MethodCandidate target miRNAs related to LNM in EEC were screened by three methods including differentially expressed miRNAs (DEmiRs), weighted gene co-expression network analysis (WGCNA), and decision tree algorithms. Samples were randomly divided into the training and validation cohorts. A miRNA signature was built using a logistic regression model and was evaluated by the area under the curve (AUC) of receiver operating characteristic curve (ROC) and decision curve analysis (DCA). We also conducted pathway enrichment analysis and miRNA–gene regulatory network to look for potential genes and pathways engaged in LNM progression. Survival analysis was performed, and the miRNAs were tested whether they expressed differently in another independent GEO database.ResultThirty-one candidate miRNAs were screened and a final 15-miRNA signature was constructed by logistic regression. The model showed good calibration in the training and validation cohorts, with AUC of 0.824 (95% CI, 0.739–0.912) and 0.821 (95% CI, 0.691–0.925), respectively. The DCA demonstrated the miRNA signature was clinically useful. Hub miRNAs in signature seemed to contribute to EEC progression via mitotic cell cycle, cellular protein modification process, and molecular function. MiR-34c was statistically significant in survival that a higher expression of miR-34c indicated a higher survival time. MiR-34c-3p, miR-34c-5p, and miR-34b-5p were expressed differentially in GSE75968.ConclusionThe miRNA signature could work as a noninvasive method to detect LNM in EEC with a high prediction accuracy. In addition, miR-34c cluster may be a key biomarker referring LNM in endometrial cancer.

show abstract

“…Interestingly, TP53 has been validated as a miR-125b target in several investigations by different methods, including reporter assay, western blot, and qPCR (23). However, the dysregulation of miR-125b and TP53 between ectopic and eutopic endometrial samples has rarely been investigated (28).…”

Section: Introductionmentioning

confidence: 99%

Investigating the expressions of miRNA-125b and TP53 in endometriosis. Does it underlie cancer-like features of endometriosis? A case-control study

Hajimaqsoudi

Darbeheshti

Kalantar

et al. 2020

IJRM

Self Cite

View full text Add to dashboard Cite

Background: Endometriosis is generally considered as a benign condition; however, there is a possibility for it to become cancerous. miR-125b is upregulated in both endometriotic tissues and serum samples of women with endometriosis but its potential targets in endometriosis are still not fully understood. Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in both eutopic (Eu-p) and ectopic endometrium (Ec-p) in the endometrium tissues of women with endometriosis was compared to those in the normal endometrium tissues of controls (Normal). Materials and Methods: In this case-control study, the Eu-p and Ec-p samples were collected from 20 women who underwent laparoscopic surgery, and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach, a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative polymerase chain reaction technique was used for the measurement of miR125b and TP53 expression. Results: Our results showed that miR-125b was significantly overexpressed in Ec-p (p-value: 0.021). In addition, there was a significant TP53 under expression in both the Ec-p and Eu-p samples compared with the Normal tissues (p-value: 0.003). Conclusion: The negative correlation between miR-125b and TP53 as well as a noticeable decreased expression of TP53 in both Ec-p and Eu-p samples may be interpreted as the roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. In addition, these findings and bioinformatic analyses imply a possible role of miR-125b in cancer-like features of endometriosis. Key words: Endometriosis, TP53, miR-125b, Ectopic endometrium, Eutopic endometrium.

show abstract

The relationship between the expression levels of miR-135a and HOXA10 gene in the eutopic and ectopic endometrium

Cited by 12 publications

References 20 publications

Hsa-mir-135a Shows Potential as A Putative Diagnostic Biomarker in Saliva and Plasma for Endometriosis

Hsa-mir-135a Shows Potential as A Putative Diagnostic Biomarker in Saliva and Plasma for Endometriosis

Identification of a MicroRNA Signature Associated With Lymph Node Metastasis in Endometrial Endometrioid Cancer

Investigating the expressions of miRNA-125b and TP53 in endometriosis. Does it underlie cancer-like features of endometriosis? A case-control study

Contact Info

Product

Resources

About