Epigenetic Advances in Somatic Embryogenesis in Sequenced Genome Crops

Duarte-Aké, Fátima; De‐la‐Peña, Clelia

doi:10.1007/978-3-319-33705-0_6

Cited by 12 publications

(10 citation statements)

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“…One is the intervention of 14-3-3 proteins, which participate in several processes such as the development of the seeds (Zhao et al, 2015) and during the induction of SE in Carica papaya (Vale Ede et al, 2014). The other factor actively involved during the SE induction, process, and development is epigenetic (Us-Camas et al, 2014; De-la-Peña et al, 2015; Duarte-Aké and De-la-Peña, 2016).…”

Section: Other Factors Involved In Signal Transduction During the Indmentioning

confidence: 99%

“…Epigenetic modifications are an essential part of the signaling pathway that leads to changes in the genetic program of the cells and the development of somatic embryos. There is evidence that shows that changes in the chromatin are able to control totipotency in plant cells (Duarte-Aké and De-la-Peña, 2016; Kumar and van Staden, 2017). The level to which chromatin reprogramming is required before SE induction depends on several factors, such as origin of the explant, the culture medium, the genetic background of the mother plant, and especially the amount of PGR used (De-la-Peña et al, 2015).…”

Section: Epigeneticsmentioning

confidence: 99%

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Signaling Overview of Plant Somatic Embryogenesis

et al. 2019

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Somatic embryogenesis (SE) is a means by which plants can regenerate bipolar structures from a somatic cell. During the process of cell differentiation, the explant responds to endogenous stimuli, which trigger the induction of a signaling response and, consequently, modify the gene program of the cell. SE is probably the most studied plant regeneration model, but to date it is the least understood due to the unclear mechanisms that occur at a cellular level. In this review, the authors seek to emphasize the importance of signaling on plant SE, highlighting the interactions between the different plant growth regulators (PGR), mainly auxins, cytokinins (CKs), ethylene and abscisic acid (ABA), during the induction of SE. The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress. Furthermore, the role of genes encoded to transcription factors (TFs) during the embryogenic process such as the LEAFY COTYLEDON (LEC), WUSCHEL (WUS), BABY BOOM (BBM) and CLAVATA (CLV) genes, Arabinogalactan-proteins (AGPs), APETALA 2 (AP2) and epigenetic factors is discussed.

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Section: Other Factors Involved In Signal Transduction During the Indmentioning

confidence: 99%

Section: Epigeneticsmentioning

confidence: 99%

Signaling Overview of Plant Somatic Embryogenesis

et al. 2019

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“…This gene is orthologous to ARF8 in rice. Plant genomes encode several AUXIN RESPONSE FACTORs ( ARF ), transcription factors that activate minutes after auxin stimulation, and are involved in various biological developmental processes (Duarte‐Aké & De‐la‐Peña, 2016). Partially redundant, ARF6 and ARF8 regulate the maturation of the stamen and the gynoecium by regulating the expression of several developmental genes that coordinate the transition from immature to mature fertile flowers (Nagpal et al., 2005).…”

Section: Resultsmentioning

confidence: 99%

The commitment of barley microspores into embryogenesis correlates with miRNA‐directed regulation of members of the SPL, GRF and HD‐ZIPIII transcription factor families

et al. 2020

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Microspore embryogenesis is a model for developmental plasticity and cell fate decisions. To investigate the role of miRNAs in this development, we sequenced sRNAs and the degradome of barley microspores collected prior to (day 0) and after (days 2 and 5) the application of a stress treatment known to induce embryogenesis. Microspores isolated at these timepoints were uniform in both appearance and in their complements of sRNAs. We detected 68 miRNAs in microspores. The abundance of 51 of these miRNAs differed significantly during microspore development. One group of miRNAs was induced when the stress treatment was applied, prior to being repressed when microspores transitioned to embryogenesis. Another group of miRNAs were up‐regulated in day‐2 microspores and their abundance remained stable or increased in day‐5 microspores, a timepoint at which the first clear indications of the transition toward embryogenesis were visible. Collectively, these miRNAs might play a role in the modulation of the stress response, the repression of gametic development, and/or the gain of embryogenic potential. A degradome analysis allowed us to validate the role of miRNAs in regulating 41 specific transcripts. We showed that the transition of microspores toward the embryogenesis pathway involves miRNA‐directed regulation of members of the ARF, SPL, GRF, and HD‐ZIPIII transcription factor families. We noted that 41.5% of these targets were shared between day‐2 and day‐5 microspores while 26.8% were unique to day‐5 microspores. The former set may act to disrupt transcripts involved in pollen development while the latter set may drive the commitment to embryogenesis.

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“…Therefore, the different values of methylated cytosine observed here and their variations during the ISE may be related to the improvement methods that gave rise to these C. arabica lines and/or the adaptation processes of the cells to environmental in vitro conditions. Cell changes to the in vitro environment of ISE are accompanied by chromatin remodeling, which influences the gene expression [47,48]. In addition, cytosine methylation is associated with genome stability by suppressing the transposition of the mobile DNA elements in plants [49].…”

Section: Discussionmentioning

confidence: 99%

Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in Coffea arabica Lines: Insights about the Different In Vitro Responses

Oliveira

Sanglard

Ferreira

et al. 2021

Forests

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Coffea arabica genotypes present distinct responses in vitro, and somaclonal variation occurrence has been reported. Global cytosine methylation is one of the epigenetic mechanisms that influences the Coffea in vitro responses. We aimed to establish the indirect somatic embryogenesis in C. arabica ‘Catuaí Vermelho’, ‘Caturra’ and ‘Oeiras’, associate the distinct responses to the methylated cytosine genomic level, and check the ploidy stability. Leaf explants were cultured in callus induction and proliferation medium. The resulted calli were transferred to the regeneration medium, and the mature cotyledonary somatic embryos were transferred to the seedling medium. ‘Oeiras’ exhibited the highest number of responsive leaf explants, followed by ‘Caturra’ and ‘Catuaí Vermelho’. Global methylated cytosine level increased over time in the ‘Catuaí Vermelho’ and ‘Caturra’ friable calli, remaining constant in ‘Oeiras’. ‘Oeiras’ did not regenerate somatic embryos, while ‘Catuaí Vermelho’ exhibited the highest number. Somatic embryo regeneration was associated with the increase of the methylated cytosine level. However, the ‘Catuaí Vermelho’ embryogenic calli showed a lower methylated cytosine level than ‘Caturra’. Recovered plantlets exhibited the same 2C value and chromosome number to the explant donors. Therefore, cytosine hypermethylation occurred during C. arabica indirect somatic embryogenesis, influencing cell competence and somatic embryos regeneration.

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Epigenetic Advances in Somatic Embryogenesis in Sequenced Genome Crops

Cited by 12 publications

References 122 publications

Signaling Overview of Plant Somatic Embryogenesis

Signaling Overview of Plant Somatic Embryogenesis

The commitment of barley microspores into embryogenesis correlates with miRNA‐directed regulation of members of the SPL, GRF and HD‐ZIPIII transcription factor families

Genomic Methylated Cytosine Level during the Dedifferentiation and Cellular Competence in Coffea arabica Lines: Insights about the Different In Vitro Responses

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