Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells

Zhang, Yunjuan; Duan, Wei; Owusu, Lawrence; Wu, Dongcheng; Xin, Yi

doi:10.3892/ijmm.2014.1988

Cited by 55 publications

(29 citation statements)

References 34 publications

(35 reference statements)

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“…Previous studies reported that several plant extracts and active compounds such as Tulbaghia violacea , Costus speciosus , epigallocatechin-3-gallate ( EGCG ) and silybin induced caspase-3 mediated apoptotic cell death on HCC. [26272829] The results obtained from the current study also confirmed that the T. purpurea extracts have the ability to cause apoptosis-mediated cell death in HepG2 cells through caspase-3.…”

Section: Discussionsupporting

confidence: 78%

In vitro anti-proliferative effect of Tephrosia purpurea on human hepatocellular carcinoma cells

et al. 2017

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Background:Tephrosia purpurea is an Indian herb used in traditional medicine to treat various diseases such as jaundice, asthma, liver and urinary disorders. However, the anti-cancer potential of T. purpurea on hepatocellular carcinoma (HCC) is poorly understood. Therefore, this study aims to investigate the anti-cancer activity of T. purpurea in HepG2 hepatocellular carcinoma cells.Methods:The leaves and root of T. purpurea were extracted with methanol using soxhlet apparatus. The cytotoxicity of the T. purpurea extracts in HepG2 cells was evaluated using MTT assay whereas the mode of cell death was examined by AOEB, Hoechst and JC1 staining under a fluorescence microscope. T. purpurea extracts-induced caspase-3 expression was investigated using colorimetric assay.Results:The leaves and root extracts inhibited HepG2 cell growth at the IC50 of 102.33 ± 10.26 µg/mL and 276.67 ± 20.43 µg/mL respectively at 24 h. Chromatin condensation, nuclear fragmentation, apoptotic bodies formation and mitochondrial membrane depolarization were observed in HepG2 cells treated with both extracts. The caspase-3 expression was significantly (p < 0.05) increased in extracts treated cells when compared to control.Conclusion:The leaves and root extracts of T. purpurea induce apoptosis mediated cell death in HepG2 cells.SUMMARYThe leaves and root extracts of T. purpurea exhibited anticancer activity in HepG2 hepatocellular carcinoma cells. These extracts induced cell shrinkage, DNA condensation and fragmentation, mitochondrial membrane depolarization and upregulated caspase-3 expression indicating T. purpurea extracts induce apoptosis in HepG2 cells.Abbreviation used: AO: acridine orange, DMSO: dimethyl sulfoxide, EB: ethidium bromide, IC50: the concentration at which 50% of cancer cells are dead, JC-1: 5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethyl-imidacarbocyanine iodide, MTT: 3-4, 5-dimethylthiazole-2-yl, 2,5-diphenyl tetrazolium bromide, PBS: phosphate-buffered saline, ΔΨm: mitochondrial trans-membrane potential.

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Section: Discussionsupporting

confidence: 78%

In vitro anti-proliferative effect of Tephrosia purpurea on human hepatocellular carcinoma cells

et al. 2017

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“…This was a noteworthy result as one of the product components is epigallocatechin-3-gallate, which has formerly been reported to induce apoptosis in HCC cells (30). The reason for this discrepancy remains unknown, but may be associated with the distinct experimental conditions used in these studies, including the doses of the products employed.…”

Section: Discussionmentioning

confidence: 68%

“…The reason for this discrepancy remains unknown, but may be associated with the distinct experimental conditions used in these studies, including the doses of the products employed. While OOS did not induce a noticeable effect on apoptosis, it exhibited a clear effect on cell cycle progression and that is a possible mechanism underlying the antitumoral action of OOS (30). The effect of OOS on cell cycle progression was more evident when analyzing the action of OOS on synchronized cells.…”

Section: Discussionmentioning

confidence: 99%

“…Firstly, our previous studies demonstrated the antitumoral value of OOS in highly proliferating cells, including breast cancer and acute myeloid leukemia (13,20). Secondly, numerous reports have demonstrated the effects of epigallocatechin-3-gallate on HCC models inducing cell death (30), suppressing hepatocellular carcinoma growth or dissemination. The present study, using two HCC cell lines, observed that OOS induced a decrease in MTT metabolization, indicative of reduced proliferation in the HCC-derived cell lines tested.…”

Section: Discussionmentioning

confidence: 99%

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Antitumoral effect of Ocoxin in hepatocellular carcinoma

et al. 2017

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Hepatocellular carcinoma (HCC) is becoming one of the most prevalent types of cancer worldwide. The most efficient types of treatment at present include surgical resection and liver transplantation, but these treatments may only be used in a small percentage of patients. In order to identify novel therapeutic strategies for this disease, the present study explored the potential antitumoral effect of Ocoxin® oral solution (OOS) in HCC. OOS inhibited the proliferation of HCC cell lines in a time- and dose-dependent manner, being more efficient when used in combination with sorafenib, a standard of care treatment for patients diagnosed with advanced-stage disease. Mechanistic studies indicated that the effect of OOS was due to the induction of cell cycle arrest rather than the stimulation of apoptotic cell death. The cell cycle was slowed down in all phases in the HCC cell lines treated with OOS. Finally, when tested in animal models of HCC, OOS reduced tumor progression through the induction of necrosis in xenograft tumor models. Considering the poor prognosis and high resistance to antitumor treatments of HCC, the antiproliferative action of OOS, particularly in combination with sorafenib, provides the opportunity to investigate the effect of combined therapy in a clinical setting.

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“…Furthermore, it dose-dependently inhibits MMP-2 activity, which is a major enzyme for the invasion process, and migration of HuCC-T1 cells as shown in Figure 3. Zhang et al also reported that EGCG efficiently inhibits MMP-2/9 activity and invasion of hepatocellular carcinoma cells 28,29. Furthermore, they also reported that EGCG has no adverse effects against non-cancerous liver cells.…”

Section: Discussionmentioning

confidence: 94%

Anticancer activities of epigallocatechin-3-gallate against cholangiocarcinoma cells

Kwak¹,

Park²,

Kim³

et al. 2016

OTT

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PurposeEpigallocatechin-3-gallate (EGCG) is an antioxidant agent derived from green tea. Because it has chemopreventive and anti-invasive effect against various cancer cells, EGCG can be used to inhibit proliferation and invasion of cholangiocarcinoma (CCA) cells.MethodsThe anticancer effects of EGCG were studied using human CCA cells (HuCC-T1). Apoptosis was analyzed by Western blotting. Invasion and migration of cancer cells were assessed with Matrigel® and wound healing assays. An animal tumor xenograft model of HuCC-T1 was used to study the in vivo antitumor activities of EGCG.ResultsEGCG effectively inhibited the growth of HuCC-T1 cells with no adverse effects on the viability of 293T cells. EGCG induced apoptotic cell death at 5 µg/mL concentration. It inhibited the expression of mutant p53 and induced apoptotic molecular signals such as Bax/Bcl-2, Caspase, and cytochrome C. Furthermore, EGCG dose-dependently inhibited the activity of matrix metalloproteinase (MMP)-2/9, invasion, and migration. In the animal tumor xenograft model of HuCC-T1 cells, EGCG was subcutaneously administered beside the tumor for local treatment. EGCG efficiently inhibited growth of the tumor and suppressed carcinogenic molecular signals such as Notch1, MMP-2/9, and proliferating cell nuclear antigen.ConclusionEGCG induced apoptosis of cancer cells without adverse effects on normal cells. EGCG inhibited growth, invasion, and migration of HuCC-T1 cells. We suggest EGCG as a promising candidate for local treatment of CCA.

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Epigallocatechin-3-gallate induces the apoptosis of hepatocellular carcinoma LM6 cells but not non-cancerous liver cells

Cited by 55 publications

References 34 publications

In vitro anti-proliferative effect of Tephrosia purpurea on human hepatocellular carcinoma cells

In vitro anti-proliferative effect of Tephrosia purpurea on human hepatocellular carcinoma cells

Antitumoral effect of Ocoxin in hepatocellular carcinoma

Anticancer activities of epigallocatechin-3-gallate against cholangiocarcinoma cells

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