Epidermal localization and protective effects of topically applied superoxide dismutase

Filipe, Paulo; Emerit, I; Vassy, Jany; Rigaut, Jean Paul; Martin, E; Freitas, João; Fernandes, A. D. Lemos

doi:10.1111/j.1600-0625.1997.tb00157.x

Cited by 21 publications

(8 citation statements)

References 18 publications

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“…Photoaging signs are more evident in most women on 2 nd -3 rd decade of life (Fisher et al, 1996). Other literature stated that extrinsic aging begins in the second decade (Filipe et al, 1997).…”

Section: Discussionmentioning

confidence: 96%

The effectiveness of Oral Superoxide Dismutase (SOD) on total antioxidant status, Trans Epidermal Water Loss (TEWL) and sebum concentration in photo aging skin

Djawad

2021

RSD

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Introduction: Aging is a progressive process of decrease in organs functions and capacity, including the skin. Photoaging is extrinsic aging mainly occur due to ultraviolet (UV) exposure. Methods: This study is a clinical trial research design with one group pre-post test. All subjects who were exposed to UV for approximately 3-4 hours. All subjects signed an informed consent and were interviewed accordingly. Photoaging was diagnosed clinically by three dermatologists according to Glogau type II classification such as dynamic wrinkles, palpable keratosis, visible lentigo senilis, and smiley line. SOD 250 IU was given to all subjects twice daily for 60 days. Laboratory examinations such as TAS, TEWL, and sebum concentration were done pre and post-intervention. Results: A total of 25 subjects, Fitzpatrick skin type 4 were included in this study. There were 14 males and 11 females with 20 subjects age 30-40 years old and 5 subjects age 25-29 years old. Fourteen (56%) out of 18 subjects from low TAS group have normal TAS post-treatment with SOD. McNemar test showed a significant increase in TAS value pre and post-treatment with SOD (p<0.05). TEWL measurement on cheek showed 9 out of 10 subjects from the strained group have normal TEWL post-treatment, while all 3 subjects from the critical group have normal TEWL value. Measurement on the forehead showed 7 subjects from the strained group have a normal TEWL. Sebumeter on the forehead showed 17 subjects from dry skin group 14 (56%) subjects have normal skin, 1 (4%) subject becomes oily, and 2 subjects remains dry post-treatment with SOD for 60 days. All subjects with dry skin on U zone become normal skin post-treatment. Conclusion: SOD significantly increased TAS value, decreased TEWL, and improvement of skin dryness post-treatment with SOD for 60 days.

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Section: Discussionmentioning

confidence: 96%

The effectiveness of Oral Superoxide Dismutase (SOD) on total antioxidant status, Trans Epidermal Water Loss (TEWL) and sebum concentration in photo aging skin

Djawad

2021

RSD

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“…Since Cu, Zn-SOD increases in response to an accumulation of ROS this increase would confirm the protective role of the enzyme against radiation-induced skin damage. In fact, some studies show that the pre-treatment of skin with topical SOD protects the skin from inflammatory reactions induced by UVA rays (12).…”

Section: Discussionmentioning

confidence: 99%

Copper/Zinc-Superoxide Dismutase in Human Epidermis: An Immunochemical Study

2019

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The localization of copper and zinc-superoxide dismutase in normal and neoplastic human skin was examined with immunochemical techniques. Skin samples were taken from males and females of different ages, UV exposed and non-exposed areas and basal-/spino-cellular carcinomas. The enzyme was localized diffusely in the cytoplasm and was also found in the nuclei of epidermal cells, endothelial cells and other dermis cell types. The dismutase content in the epidermis was higher in males than females, UV-exposed than non-exposed and young than old people. In the tumors, the enzyme content of the superficial epidermal layers was higher than in the deep tumoral epithelial cells. These data suggest that the localization of Cu, Zn-SOD in skin tissues reflects the gender and age of the subject, the cell types and their normal or diseased state. Further studies based on the investigation of systemic changes of this enzyme in physiological and pathological epidermis could provide additional information on tumor cell generation.

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“…From these results, it is considered that severe oxidative stress due to induced ROS generation, such as that by photooxidative stress, damages the cellular and tissue components of the skin more severely at advancing ages than at younger ages. Therefore, we emphasize that application of antioxidants is useful to protect the skin from accumulated and elevated oxidative damage due to UV light from the sun as the aging process proceeds (41,50,51).…”

Section: Discussionmentioning

confidence: 99%

Age‐dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light

Yasui

2003

Experimental Dermatology

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Aging proceeds by highly complicated biochemical processes, in which the involvement of the reactive oxygen species (ROS) and free radicals has been implicated. Although the relationship between UV-induced photoaging and ROS generation has been proposed, it has been difficult to establish direct proof of the generation of ROS in the skin under UV exposure. Recently, we reported finding endogenously generated ROS in the skin of live mice after UVA light exposure by a method of in vivo chemiluminescent detection, in which superoxide anion radical (*O2-) and singlet oxygen species (1O2) are contributed. In light of the results, we tried to understand the age-dependent changes in ROS generation in the skin of hairless rats under UVA exposure. Chemiluminescent levels due to ROS in the untreated and UVA-exposed skin decreased age dependently, and the signal intensities in old rats were significantly lower than those in young rats. However, the ratios of chemiluminescent intensities in the UVA-exposed skin to those in the untreated skin were significantly enhanced in an age-dependent manner. These results suggest that the antioxidative ability against ROS generation in the skin, possessed by antioxidant enzymes and low molecular weight antioxidants, is lowered age dependently.

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Epidermal localization and protective effects of topically applied superoxide dismutase

Cited by 21 publications

References 18 publications

The effectiveness of Oral Superoxide Dismutase (SOD) on total antioxidant status, Trans Epidermal Water Loss (TEWL) and sebum concentration in photo aging skin

The effectiveness of Oral Superoxide Dismutase (SOD) on total antioxidant status, Trans Epidermal Water Loss (TEWL) and sebum concentration in photo aging skin

Copper/Zinc-Superoxide Dismutase in Human Epidermis: An Immunochemical Study

Age‐dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light

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