Epidermal growth factor stimulates proliferation and migration of porcine trophectoderm cells through protooncogenic protein kinase 1 and extracellular-signal-regulated kinases 1/2 mitogen-activated protein kinase signal transduction cascades during early pregnancy

Jeong, Wooyoung; Kim, Jin‐Young; Bazer, Fuller W.; Song, Gwonhwa

doi:10.1016/j.mce.2013.08.024

Cited by 37 publications

(25 citation statements)

References 51 publications

(54 reference statements)

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“…Moreover, they have been implicated in the differentiation, migration and cytoskeletal remodeling of embryo trophoblast cells during the preimplantation period (64,65). In addition, studies in different species have agreed that deficiencies in MAPK/ERK proteins lead to early embryonic death due to the lack of signal transduction for proliferation and invasion of trophoblasts (66)(67)(68). Another interesting pathway found to be upregulated in our study was the Wnt signaling pathway.…”

Section: Discussionsupporting

confidence: 62%

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

et al. 2019

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Results: Embryo viability was equal between the groups (∼93%), but embryo development was significantly (P < 0.05) more advanced in the SP-treated group compared to control. A total of 1,604 endometrium transcripts were differentially expressed in the SP group compared to the control group. An enrichment analysis showed an overrepresentation of genes and pathways associated with the immune response, cytokine signaling, cell cycle, cell adhesion, and hormone response, among others.Conclusions: SP infusions prior to AI positively impacted the preimplantation embryo development and altered the expression of the endometrial genes and pathways potentially involved in embryo development.

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Section: Discussionsupporting

confidence: 62%

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

et al. 2019

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“…Opening the window of receptivity for conceptus attachment to the uterine endometrium (Day 10 to 14) following down-regulation of the uterine epithelial PGR marks a period of conceptus growth, development and change in morphology stimulated by the release of multiple uterine growth factors and cytokines [ 2 – 4 ]. During the early peri-implantation period, the endometrium increases the release of epidermal growth factor (EGF) [ 34 – 37 ], insulin-like growth factor-1 (IGF-1) [ 38 – 42 ], FGF7 [ 43 , 44 ], vascular endothelial growth factor (VEGF) [ 45 – 47 ], interleukin 6 (IL6) [ 48 – 50 ], transforming growth factor beta (TGFB) [ 51 – 53 ], and leukemia inhibitory factor (LIF) [ 48 – 50 ] for which the developing conceptus trophectoderm expresses EGF-receptor (EGFR) [ 36 ], IGF1R [ 54 ], FGFR2 [ 55 ], VEGFR1 and 2 [ 45 , 47 ], IL6R [ 50 ], TGFBR1 and 2 [ 52 ], and LIFR [ 50 ]. The increased endometrial release of EGF, FGF7, LIF, and IGF-1 are enhanced in the epithelium during the period of conceptus elongation and estrogen release [ 42 , 44 , 50 , 51 , 55 ].…”

Section: Introductionmentioning

confidence: 99%

Cytokines from the pig conceptus: roles in conceptus development in pigs

Geisert

Lucy

Whyte

et al. 2014

J Animal Sci Biotechnol

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Establishment of pregnancy in pigs involves maintaining progesterone secretion from the corpora lutea in addition to regulating a sensitive interplay between the maternal immune system and attachment of the rapidly expanding trophoblast for nutrient absorption. The peri-implantation period of rapid trophoblastic elongation followed by attachment to the maternal uterine endometrium is critical for establishing a sufficient placental-uterine interface for subsequent nutrient transport for fetal survival to term, but is also marked by the required conceptus release of factors involved with stimulating uterine secretion of histotroph and modulation of the maternal immune system. Many endometrial genes activated by the conceptus secretory factors stimulate a tightly controlled proinflammatory response within the uterus. A number of the cytokines released by the elongating conceptuses stimulate inducible transcription factors such as nuclear factor kappa B (NFKB) potentially regulating the maternal uterine proinflammatory and immune response. This review will establish the current knowledge for the role of conceptus cytokine production and release in early development and establishment of pregnancy in the pig.Electronic supplementary materialThe online version of this article (doi:10.1186/2049-1891-5-51) contains supplementary material, which is available to authorized users.

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“…The PI3K/AKT signaling pathway is a crucial for migration, differentiation and survival of cells (Franke et al, ). Especially, the PI3K/AKT cascade is activated by numerous growth factors, hormones and nutrients during the peri‐implantation period of pregnancy in rodents and other mammals (Riley et al, ; Jeong et al, ). As a primary target, AKT transduces growth signals to the embryos and a deficiency of AKT increases perinatal mortality with growth retardation and insufficient placentation (Yang et al, ).…”

Section: Discussionmentioning

confidence: 99%

Stimulatory Effects of Coumestrol on Embryonic and Fetal Development Through AKT and ERK1/2 MAPK Signal Transduction

Lim

Song

2016

Journal Cellular Physiology

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Successful establishment of pregnancy is required for fetal-maternal interactions regulating implantation, embryonic development and placentation. A uterine environment with insufficient growth factors and nutrients increases the incidence of intrauterine growth restriction (IUGR) leading to an impaired uterine environment. In the present study, we demonstrated the effects of the phytoestrogen coumestrol on conceptus development in the pig that is regarded as an excellent biomedical animal model for research on IUGR. Results of this study indicated that coumestrol induced migration of porcine trophectoderm (pTr) cells in a concentration-dependent manner. In response to coumestrol, the phosphorylation of AKT, P70S6K, S6, ERK1/2 MAPK, and P90RSK proteins were activated in pTr cells and ERK1/2 MAPK and P90RSK phosphorylation was prolonged for a longer period than for the other proteins. To identify the signal transduction pathway induced by coumestrol, pharmacological inhibitors U0126 (an ERK1/2 inhibitor) and LY294002 (a PI3K inhibitor) were used to pretreat pTr cells. The results showed that coumestrol-induced phosphorylation of ERK1/2 MAPK and P90RSK was blocked by U0126. In addition, the increased phosphorylation in response to coumestrol was completely inhibited following pre-treatment incubation of pTr cells in the presence of LY294002 and U0126. Furthermore, these two inhibitors suppressed the ability of coumestrol to induce migration of pTr cells. Collectively, these findings suggest that coumestrol affects embryonic development through activation of the PI3K/AKT and ERK1/2 MAPK cell signal transduction pathways and improvement in the uterine environment through coumestrol supplementation may provide beneficial effects of enhancing embryonic and fetal survival and development. J. Cell. Physiol. 231: 2733-2740, 2016. © 2016 Wiley Periodicals, Inc.

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Epidermal growth factor stimulates proliferation and migration of porcine trophectoderm cells through protooncogenic protein kinase 1 and extracellular-signal-regulated kinases 1/2 mitogen-activated protein kinase signal transduction cascades during early pregnancy

Cited by 37 publications

References 51 publications

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

Seminal Plasma Modifies the Transcriptional Pattern of the Endometrium and Advances Embryo Development in Pigs

Cytokines from the pig conceptus: roles in conceptus development in pigs

Stimulatory Effects of Coumestrol on Embryonic and Fetal Development Through AKT and ERK1/2 MAPK Signal Transduction

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