Epidemiology of diarrhoea caused by rotavirus and Escherichia coli in lambs in Kashmir valley, India

Abstract

One hundred and twenty-nine faecal samples, collected over a period of 1 year, from 96 diarrhoeic and 33 non-diarrhoeic lambs aged between 0 and 3 months were examined for presence of rotavirus and Escherichia coli (E. coli). Group A rotavirus was detected in 24 (25%) of diarrhoeic lambs using sandwich enzyme linked immunosorbent assay (ELISA) and ribonucleic acid-polyacrylamide gel electrophoresis (RNA-PAGE). Statistically no significant relation was found between rotavirus infection and age of the lambs. The prevalence of group A rotavirus was more related to meterological changes than age of the lambs as the number of diarrhoeic lambs with rotavirus infection was found to increase in spring months during which temperature and humidity ranged between 7.34 and 28.9 • C and 34.28 and 82.58%, respectively. The migration pattern of ovine rotavirus RNA in PAGE was typical of mammalian group A rotaviruses. O25, O26, O30, O43, O75, O76, O102, O113, O132, O153 and O157 E. coli serogroups were isolated from diarrhoeic lambs positive for rotavirus infection while as O157 serogroups of E. coli were isolated from diarrhoeic lambs without rotavirus infection. O8, O21, O43, O82, O104, O113, O120, O127, O132 and O139 serogroups, recovered from diarrhoeic faecal samples with or without rotavirus, were positive for congo red dye binding activity. O88, O113, O157 and O168 serogroups were isolated from non-diarrhoeic faecal samples out of which O88 and O168 were congo red positive. None of the lambs without diarrhoea carried rotavirus infection. Group B rotavirus infection commonly reported in diarrhoeic lambs outside India was not detected in any of the faecal samples screened.

Identification, characterization and molecular epidemiology of Escherichia coli isolated from lamb and goat kids with diarrhoea

“…Clonal spread of a common strain was not detected among the animals tested in the present study. in small ruminants from Turkey (Gokce et al 2010) and other states (Wani et al 2004), however, research on classification of enterovirulent E. coli and its virulence genes is limited. Recent studies have reported (Wani et al 2003;Bhat et al 2008;Bandyopadhyay et al 2011), the existence of at least one virulence gene in 41%, 36.7%, and 32.6% of E. coli isolates, respectively.…”

Virulence repertoire of Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic Escherichia coli (ETEC) from diarrhoeic lambs of Arunachal Pradesh, India

“…Previously, E. coli was isolated from 58% diarrhoeic lambs in India (Wani et al 2004b). Of the 234 isolates, only 74 strains (32%) carry at least one virulence gene characteristic for STEC.…”

Avian rotavirus enteritis – an updated review

“…Although RV causes diarrhea in different species like human infants, calves, lambs, goat kids, piglets and puppies in different parts of the world (Georges et al 1984, Garcia et al 2000, Wani et al 2004Dhama et al 2009), reports regarding circulation of AvRVs are scarce in India. Wani et al (2003) first detected the mammalianlike electropherogroup AvRVs from chicken with diarrhea.…”