Epidemiology and echinocandin susceptibility of Candida parapsilosis sensu lato species isolated from bloodstream infections at a Spanish university hospital

García‐Effrón, Guillermo; Cantón, Emilia; Pemán, Javier; Dilger, Amanda; Romá, Eva; Perlin, David S.

doi:10.1093/jac/dks271

Cited by 37 publications

(19 citation statements)

References 55 publications

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“…17,31 According to this study C. parapsilosis isolates showed intermediate susceptibility to micafungin, corroborating previous findings that suggest a decreased susceptibility in C. parapsilosis. 3,12,17 The results of the present study show that the MIC values for amphotericin B among the three species of the Parapsilosis complex were similar. However, Lockhart et al 17 observed higher MIC values for C. parapsilosis.…”

Section: Discussionsupporting

confidence: 57%

In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

Herkert

Gomes

Muro

et al. 2015

Revista Iberoamericana de Micología

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Section: Discussionsupporting

confidence: 57%

In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

Herkert

Gomes

Muro

et al. 2015

Revista Iberoamericana de Micología

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“…Recent works have described a functional point mutation in the glucan synthase (FKS) gene of the C. parapsilosis complex species, which may explain this observation (46) (47) . FKS gene mutations have been shown to be associated with resistance to CAS, as demonstrated by increases in MICs in mutant isolates compared with those of wild-type isolates (48) (49) (50) . In summary, our study reported the first molecular identifi cation of C. parapsilosis complex species in State of Goiás, Brazil.…”

Section: In Vitro Susceptibility Testingmentioning

confidence: 99%

Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

Ataídes

Costa

Souza

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction:Candida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. In this study, we differentiated Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis from a culture collection containing blood and subungual scraping samples. Furthermore, we assessed the in vitro antifungal susceptibility of these species to fl uconazole, itraconazole, voriconazole, posaconazole, amphotericin B, and caspofungin. Methods: Differentiation of C. parapsilosis complex species was performed by amplifi cation of the secondary alcohol dehydrogenase (SADH) gene and digestion by the restriction enzyme BanI. All isolates were evaluated for the determination of minimal inhibitory concentrations using Etest, a method for antifungal susceptibility testing. Results: Among the 87 isolates, 78 (89.7%) were identifi ed as C. parapsilosis sensu stricto, fi ve (5.7%) were identifi ed as C. orthopsilosis, and four (4.6%) were identifi ed as C. metapsilosis. Analysis of antifungal susceptibility showed that C. parapsilosis sensu stricto isolates were less susceptible to amphotericin B and itraconazole. One C. parapsilosis sensu stricto isolate was resistant to amphotericin B and itraconazole. Moreover, 10.2% of C. parapsilosis sensu stricto isolates were resistant to caspofungin. Two C. parapsilosis sensu stricto isolates and one C. metapsilosis isolate were susceptible to fl uconazole in a dose-dependent manner. Conclusions: We reported the fi rst molecular identifi cation of C. parapsilosis complex species in State of Goiás, Brazil. Additionally, we showed that although the three species exhibited differences in antifungal susceptibility profi les, the primary susceptibility of this species was to caspofungin.

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“…If Candida albicans remains the most frequent bloodstream fungal pathogen, but non-albicans species are increasing and are associated with variable susceptibility profiles to antifungal drugs (5). Recently, new cryptic Candida species were described (i.e., C. metapsilosis and C. orthopsilosis in the C. parapsilosis complex or C. nivariensis and C. bracarensis in the C. glabrata complex), species that conventional phenotypic identification methods cannot identify (6,7). Additionally, invasive infections due to the less frequent and naturally resistant to echinocandins species of the genera Cryptococcus, Trichosporon, Malassezia, and Rhodotorula have been observed (8).…”

mentioning

confidence: 99%

Comparison between the Biflex III-Biotyper and the Axima-SARAMIS Systems for Yeast Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2013

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). Species were identified by using routine conventional phenotypical methods and internal transcribed spacer (ITS) sequencing in case of discrepancy. We used expanded thresholds for species identification (log score of >1.7 with 3 identical consecutive propositions and no discrepancy between the duplicates for the Bruker Daltonics system and similitude of >40% with 5 successive identical propositions and no discrepancy between the duplicates for the Shimadzu system). Of the 312 isolates, 272 (87.2%) and 258 (82.7%) were successfully identified by the Bruker Daltonics and Shimadzu systems, respectively. All isolates were successfully identified within the most frequent and clinically relevant Candida species by the two systems. Nonvalid results corresponded mainly to species not or poorly represented in the databases. Major misidentifications were observed for 2 isolates (0.6%) by the Bruker Daltonics system and 4 isolates (1.3%) by the Shimadzu system. In conclusion, the performances of the Bruker Daltonics and the Shimadzu systems for yeast identification were good and comparable under routine clinical conditions, despite their differences in sample preparation, database content, and spectrum analysis.

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Epidemiology and echinocandin susceptibility of Candida parapsilosis sensu lato species isolated from bloodstream infections at a Spanish university hospital

Cited by 37 publications

References 55 publications

In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

Comparison between the Biflex III-Biotyper and the Axima-SARAMIS Systems for Yeast Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

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