In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

Herkert, Patricia Fernanda; Gomes, Renata Rodrigues; Muro, Marisol Dominguez; Pinheiro, Rosângela Lameira; Fornari, Gheniffer; Vicente, Vânia A.; Queiroz‐Telles, Flávio

doi:10.1016/j.riam.2014.07.007

Cited by 13 publications

(7 citation statements)

References 35 publications

(36 reference statements)

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“…The phylogeny of C. parapsilosis complex based on BT2 gene is similar to the one inferred by using HWP1 gene [26], D1/D2 region of ribosomal RNA gene [ 32 ], ITS, and 26S rRNA gene [ 33 ] sequences confirming the placement of C. parapsilosis and C. orthopsilosis in ’ psilosis ’ clade.…”

Section: Discussionsupporting

confidence: 61%

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Kharazi¹,

Ahmadi²,

Makimura³

et al. 2018

CMM

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Background and Purpose: Candida parapsilosis is a common cause of candidemia in children and patients with onco-hematological diseases, septic arthritis, peritonitis, vaginitis, and nail and skin infections. Regarding this, the present study was condcuted to evaluate intra- and inter-species variation within beta-tubulin DNA sequence of C. parapsilosis complex in order to establish the utilization of this gene in the identification and phylogenetic analysis of the species.Materials and Methods:A total of 23 isolates representing three different species of C. parapsilosis complex were used in this study, all of which were identifed by ITS-sequencing. For the successful amplification of beta-tubulin gene, a newly designed set of pan-Candida primers was used, followed by bilaterally sequence analysis for pairwise comparisons, determination of multiple alignments, evaluation of sequence identity levels, counting sequence difference, and construction of phylogenetic tree.Results:The multiple alignment of 623-629 bp-long nucleotide (nt) sequences reflecting the beta-tubulin gene indicated an inter-species divergence ranging within 0-68 nt in C. parapsilosis, C. orthopsilosis, and C. metapsilosis with a mean similarity of 84.7% among the species. Meanwhile, the intra-species differences of 0-20 and 0-6 nt were found between the strains of C. parapsilosis and C. orthopsilosis, respectively. The phylogenetic tree topology was characterized by a clade made up by C. parapsilosis and C. orthopsilosis, while C. metapsilosis formed a related but separate lineage.Conclusion:Our data provided the basis for further discoveries of the relationship between the species belonging to C. parapsilosis complex. Furthermore, the findigns of the prsent study revealed the efficiency of beta-tubulin DNA sequence data in the identification and taxonomy of C. parapsilosis and other pathogenic yeasts.

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Section: Discussionsupporting

confidence: 61%

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Kharazi¹,

Ahmadi²,

Makimura³

et al. 2018

CMM

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“…However, in our study, HWP1 gene was not amplified for C. metapsilosis . Our ﬁnding confirmed the previous analysis in which C. parapsilosis and C. orthopsilosis were placed in " psilosis " clade [ 38 , 40 , 41 ].…”

Section: Discussionsupporting

confidence: 90%

“…In addition, the phylogeny of C. parapsilosis complex based on the HWP1 gene showed a completely similar topology with the phylogenetic tree based on the D1/D2 region of rDNA reported by Herkert et al [ 40 ] and ITS ( Figure 3 ), SADH, and 26S rRNA gene sequences presented by Mirhendi et al [ 14 ]. However, in our study, HWP1 gene was not amplified for C. metapsilosis .…”

Section: Discussionsupporting

confidence: 83%

Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis

et al. 2016

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Background and Purpose:Hyphal wall protein 1 (HWP1) is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different Candida species. The hyphal wall protein-coding gene (HWP1) was evaluated as a novel identiﬁcation and phylogenetic marker in Candida tropicalis, C. orthopsilosis, C. parapsilosis and C. glabrata.Materials and Methods:Initially, four specific primer pairs were designed, and the target was amplified and finally sequenced. A total of 77 Candida isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of nucleotide and amino acid polymorphism were assessed.Results:According to the results, the specific amplified fragments of HWP1 gene were useful for the differentiation of four species. Intra-species variation was observed only in C. tropicalis with two DNA types. The phylogenetic tree of Candida species based on the HWP1 gene showed consistency in topology with those inferred from other gene sequences.Conclusion:We found that HWP1 gene was an excellent marker for the identification of non-albicans Candida species as well as the phylogenetic analysis of the most clinically significant Candida species.

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“…Candida albicans is the most prevalent species among Candida infections, but other species such as C. glabrata, C. parapsilosis, C. tropicalis , and C. krusei ( Pichia kudriavzevii/Issatchenkia orientalis ) also underlie human infections [ 8 , 9 ]. Even though C. parapsilosis currently consists of a complex of three related species, namely, C. parapsilosis sensu lato, C. orthopsilosis , and C. metapsilosis , Brazilian isolates of the latter species have been reported at a lower frequency [ 10 ]. In addition, some Candida species, like C. glabrata and C. krusei , have different degrees of susceptibility and may be resistant to antifungals that are employed in clinical practice [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

Andrade

Orlando

Scorzoni

et al. 2020

JoF

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Plants belonging to the genus Copaifera are widely used in Brazil due to their antimicrobial properties, among others. The re-emergence of classic fungal diseases as a consequence of antifungal resistance to available drugs has stimulated the search for plant-based compounds with antifungal activity, especially against Candida. The Candida-infected Caenorhabditis elegans model was used to evaluate the in vitro antifungal potential of Copaifera leaf extracts and trunk oleoresins against Candida species. The Copaifera leaf extracts exhibited good antifungal activity against all Candida species, with MIC values ranging from 5.86 to 93.75 µg/mL. Both the Copaifera paupera and Copaifera reticulata leaf extracts at 46.87 µg/mL inhibited Candida glabrata biofilm formation and showed no toxicity to C. elegans. The survival of C. glabrata-infected nematodes increased at all the tested extract concentrations. Exposure to Copaifera leaf extracts markedly increased C. glabrata cell vacuolization and cell membrane damage. Therefore, Copaifera leaf extracts are potential candidates for the development of new and safe antifungal agents.

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In vitro susceptibility and molecular characterization of Candida spp. from candidemic patients

Cited by 13 publications

References 35 publications

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Characterization of beta-tubulin DNA sequences within Candida parapsilosis complex

Hyphal wall protein 1 gene: A potential marker for the identification of different Candida species and phylogenetic analysis

Brazilian Copaifera Species: Antifungal Activity against Clinically Relevant Candida Species, Cellular Target, and In Vivo Toxicity

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