Epidemiology and antibiotic resistance trends of Pantoea species in a tertiary-care teaching hospital: A 12-year retrospective study

Abstract

Purpose Pantoea species are pigmented, Gram-negative rods belonging to the Enterobacterales order. They are considered rare, opportunistic pathogens and are mostly implicated in nosocomial outbreaks affecting neonates and immunocompromised patients. The aim of this study was to describe the prevalence and antibiotic susceptibility of Pantoea species during a 12-year period. Materials and methods This retrospective study was carried out using microbiological data collected between January 1, 2006 and December 31, 2017. Patients’ data such as age, sex, inpatient/outpatient status, and empiric antibiotic therapy were also collected. Antimicrobial susceptibility testing was performed using E-tests; the interpretation was based on European Committee on Antimicrobial Susceptibility Testing breakpoints for Enterobacterales. Results Seventy individual Pantoea spp. isolates were identified; the most frequently isolated species was Pantoea agglomerans. Most isolates were susceptible to relevant antibiotics. In 61 out of 68 patients, ampicillin was the empirically administered antibiotic. The highest levels of resistance were to amoxicillin–clavulanic acid and ampicillin. No extended spectrum beta-lactamase-positive isolate was detected. Conclusions There is a scarcity of data available on the susceptibility patterns of Pantoea species, but our results correspond to what we could find in the literature. The development of multidrug-resistant (MDR) Gram-negative bacteria is a grave concern, and the development of MDR Pantoea spp. may be expected in the future.

Leclercia adecarboxylata as an emerging pathogen in human infections: a 13-year retrospective analysis in Southern Hungary

“…Sample processing in our Institute was carried out according to guidelines for routine clinical bacteriology, which have been previously described [21]. At the time of the sample submission, relevant samples were cultured on blood agar, chocolate agar, EMB agar, MacConkey agar (bioMérieux, Marcyl'Étoile, Lyon, France) and UriSelect agar (in case of urine samples; Bio-Rad, Berkeley, CA, USA) plates and incubated at 37 °C for 24-48 hours, aerobically.…”

Rothia Bacteremia

“…in the literature will presumably increase. 26 For the empiric therapy of these infections, ceftriaxone, imipenem/cilastatin or meropenem, vancomycin, or the newer fluoroquinolones (ciprofloxacin, levofloxacin, moxifloxacin) are used. 7 Based on the reports available in the literature, most (>99%) of Rothia isolates are susceptible to penicillin G and V, aminopenicillins (ampicillin, amoxicillin), first-to third-generation cephalosporins, carbapenems, and vancomycin; the susceptibility to fluoroquinolones (ciprofloxacin and newer agents) is similarly high, estimated to be around 90% to 95%.…”

“…During the study, only the first isolation of the bacteria per patient was included in the analysis; however, isolates with different antibiotic susceptibilities from the same patient were considered as different individual isolates. 26 Time-to-positivity data corresponding to the positive blood culture bottles was also collected. 27 Polymicrobial bacteremia was defined by the isolation of more than one organism in a single blood culture.…”

Emergence of Raoultella ornithinolytica isolated from chicken products in Alexandria, Egypt

“…Then, 0.1 ml and 1 ml of pre-enriched aliquots were transferred into 10 ml Rappaport and Vassiliadis broth for the enrichment and then incubated at 42°C for 24 h (HiMedia ® , India). The enriched aliquot samples were seeded onto MacConkey and Xylose Lysine Deoxycholate agar (XLD) and incubated at 37°C for 24–48 h to observe colonial growth (HiMedia ® , India) [ 17 ].…”