Epidemiological study on Lactococcus garvieae isolates from fish in Japan

Nishiki, Issei; Furukawa, Mikiko; Matui, Sho; Itami, Toshiaki; Nakai, Toshiko; Yoshida, Terutoyo

doi:10.1007/s12562-011-0332-0

Cited by 21 publications

(24 citation statements)

References 21 publications

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“…The species L. garvieae has been isolated from several fish species worldwide, namely in Japan [27], South Africa [28], Europe [29], and Brazil. Its first outbreak was reported in 2009 [7].…”

Section: The Analysis Of the Common Bacterial Fish Pathogensmentioning

confidence: 99%

Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

Sebastião¹,

Furlan²,

Hashimoto³

et al. 2015

AiM

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Intensive fish farming systems in Brazil have increased the disease incidence, mainly of bacterial origin, due to higher stocking density, high organic matter levels and poor quality of the aquatic environment that causes high mortality rates during outbreaks. The identification of pathogenic species using a fast and reliable method of diagnosis is essential for successful epidemiological studies and disease control. The present study evaluated the use of direct colony PCR in combination with 16S rRNA gene sequencing to diagnose fish bacterial diseases, with the goal of reducing the costs and time necessary for bacterial identification. The method was successful for all 178 isolates tested and produced bands with the same intensity as the standard PCR performed using pure DNA. In conclusion, the genetics methods allowed detecting the most common and important pathogens in Aquaculture, including 12 species of occurrence in Brazilian fish farms. The results of the present study constitute an advance in the available diagnostic methods for bacterial pathogens in fish farms.

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Section: The Analysis Of the Common Bacterial Fish Pathogensmentioning

confidence: 99%

Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

Sebastião¹,

Furlan²,

Hashimoto³

et al. 2015

AiM

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“…According to an epidemiological study on L. garvieae strains isolated from diseased fish in Japan, the S1 genotype is predominant in aquaculture and accounts for more than 74% of the strains (316/427) (Nishiki et al . ). In the present study, all 30 of the examined strains of genotype S1 carried the PLgT‐1 DNA.…”

Section: Discussionmentioning

confidence: 97%

“…; Nishiki et al . ; Ghasemi, Bouzari & Emtiazi ). To effectively select bacterial strains for multiplication of these lytic phages, lysogenic bacteria need to be excluded because of the resistance to phage attachment conferred by superinfection immunity (Jiang & Paul ; Day ).…”

Section: Introductionmentioning

confidence: 99%

“…Some phages are known to be specific to L. garvieae infecting fish, and all of these phages are lytic (Park et al 1997;Nakai et al 1999;Nishiki et al 2011;Ghasemi, Bouzari & Emtiazi 2014). To effectively select bacterial strains for multiplication of these lytic phages, lysogenic bacteria need to be excluded because of the resistance to phage attachment conferred by superinfection immunity (Jiang & Paul 1998;Day 2004).…”

Section: Introductionmentioning

confidence: 99%

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Induction and characterization of a lysogenic bacteriophage of Lactococcus garvieae isolated from marine fish species

Hoai

Yoshida

2015

Journal of Fish Diseases

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This study investigated the presence of prophages in Lactococcus garvieae isolated from several marine fish species in Japan. Representative strains of 16 bacterial genotypes (S1-S16) selected from more than 400 L. garvieae isolates were used to induce lysogenic bacteriophages. These strains were treated with 500 ng mL(-1) freshly prepared mitomycin C. A cross-spotting assay was performed to validate the lysogenic and indicator strains. The lysogenic strains were selected for isolation and concentration of the phages. Phage DNA was digested with EcoRI for biased sinusoidal field gel electrophoresis analysis. Polymerase chain reaction (PCR) was used to detect integrated prophage DNA. Of the 16 representative bacterial genotypes, 12 strains integrated prophages as indicated by the PCR assay, and 10 phages were detected and isolated using two indicator bacterial strains. Analysis of genomic DNA showed that these phages were homologous and named as PLgT-1. Transmission electron microscopy revealed that the morphology of PLgT-1 was consistent with the virus family Siphoviridae. PCR analysis of the prophage DNA revealed that all of the S1 genotype strains were lysogenic (30/30), but none of the S16 genotype strains were lysogenic (0/30). This is the first study to investigate lysogenic bacteriophages from L. garvieae.

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“…In aquaculture, yellowtail infected with L. garvieae show high mortality (1–3). A protective vaccine containing formalin-inactivated cells, which can be injected or orally administered, was developed for the genus Seriola , dramatically decreasing the damage caused by L. garvieae infection (4, 5).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Nonagglutinating Lactococcus garvieae Strain 122061 Isolated from Yellowtail in Japan

et al. 2016

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Epidemiological study on Lactococcus garvieae isolates from fish in Japan

Cited by 21 publications

References 21 publications

Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

Identification of Bacterial Fish Pathogens in Brazil by Direct Colony PCR and 16S rRNA Gene Sequencing

Induction and characterization of a lysogenic bacteriophage of Lactococcus garvieae isolated from marine fish species

Complete Genome Sequence of Nonagglutinating Lactococcus garvieae Strain 122061 Isolated from Yellowtail in Japan

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