Complete Genome Sequence of Nonagglutinating
            <i>Lactococcus garvieae</i>
            Strain 122061 Isolated from Yellowtail in Japan

Nishiki, Issei; Oinaka, Daisaku; Iwasaki, Yoshiaki; Yasuike, Motoshige; Nakamura, Yoji; Yoshida, Terutoyo; Fujiwara, Atushi; Nagai, Satoshi; Katoh, Masaya; Kobayashi, Takanori

doi:10.1128/genomea.00592-16

Cited by 16 publications

(4 citation statements)

References 8 publications

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“…The first strain was the most closely related to strain JJJN1 derived from the so‐iuy mullet ( Planiliza haematocheilus ) in South Korea in 2017, whereas the third strain was almost identical to strain MS210922A derived from the greater amberjack in Japan in 2021 (Akmal et al, 2022). On the contrary, L21‐20 was the most closely related to strain 122,061, isolated from the yellowtail amberjack in Japan in 2012 (Nishiki et al, 2016). The strain 122,061 was reported as a divergent serotype of L. garviae , but the phylogenetic analysis indicates that it belongs to L. formosensis .…”

Section: Resultsmentioning

confidence: 99%

Comparative genome analyses of three serotypes of Lactococcus bacteria isolated from diseased cultured striped jack (Pseudocaranx dentex)

Mahmoud

Abdelsalam

Kawato

et al. 2023

Journal of Fish Diseases

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Lactococcosis, caused by members of the genus Lactococcus, represents a devastating disease inducing mass mortalities and economic losses in many fish species worldwide. The present work aimed to compare the whole genome sequences of three different serotypes of Lactococcus garvieae isolated from diseased cultured striped jack (Pseudocaranx dentex) in Ehime prefecture, Japan. The three serotypes showed different virulence in the challenge test using Japanese amberjack (Seriola quinqueradiata). The genome sequencing revealed that two of the strains (serotype I and serotype III) were identified as L. garvieae, while the third strain (serotype II) was identified as L. formosensis. The chromosome sizes of the three serotypes ranged from 1.9 to 2.0 Mb; the GC content ranges were 38.2 to 38.9%; and the numbers of predicted protein‐coding sequences (CDSs) were from 1922 to 1959. Only the serotype II harbours two plasmids, sizes of around 14 kb and 9 kb. The detected virulence factors varied among the different serotypes with some shared factors like adherence, anti‐phagocytosis, secretion system, toxin (haemolysin), serum resistance, antimicrobial resistance and others. The genomes also contained factors responsible for resistance to toxic compounds. The genome of the serotype III tended to encode more prophage regions than the other serotypes.

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Section: Resultsmentioning

confidence: 99%

Comparative genome analyses of three serotypes of Lactococcus bacteria isolated from diseased cultured striped jack (Pseudocaranx dentex)

Mahmoud

Abdelsalam

Kawato

et al. 2023

Journal of Fish Diseases

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“…The agglutinated and non‐agglutinated strains were designated as serotype I and serotype II, respectively (Fukuda et al, 2015; Ohbayashi et al, 2017). Furthermore, average nucleotide identity (ANI) revealed that serotype I (AP090129) and serotype II (122061) were 90.7% similar to each other (Nishiki et al, 2016). Recently, a new strain, which is non‐reactive to both serotype I and II antisera has been isolated (Akmal et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Resistance and genomic characterization of a plasmid pkh2101 harbouring erm(B) isolated from emerging fish pathogen Lactococcus garvieae serotype II in Japan

Akmal

Akatsuka

Nishiki

et al. 2023

Journal of Fish Diseases

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The emergence of antibiotic‐resistant pathogenic strains of Lactococcus garvieae serotype II isolated from fish in Japan has become a growing concern in recent years. The data on drug susceptibility and its associated resistance mechanism are limited. Therefore, the present study was conducted to determine the minimum inhibitory concentrations (MICs) of chemotherapeutic agents against 98 pathogenic strains of emerging Lactococcus garvieae serotype II isolated from fish from six different prefectures in Japan from 2018 to 2021. The tested strains were resistant to erythromycin, lincomycin and tiamulin. PCR amplification revealed the presence of erm(B) in all erythromycin‐resistant strains, while a conjugation experiment confirmed that these strains carried erm(B) that could be transferred to recipient Enterococcus faecalis OG1RF with frequencies from 10−4 to 10−6 per donor cells. Nucleotide sequencing of the representative isolated plasmid pkh2101 from an erythromycin‐resistant strain showed that it was a 26,850 bp molecule with an average GC content of 33.49%, comprising 31 CDSs, 13 of which remained without any functional annotation. Comparative genomic analysis suggested that pkh2101 shared the highest similarity (97.57% identity) with the plasmid pAMbeta1, which was previously isolated clinically from Enterococcus faecalis DS‐5. This study provides potential evidence that the plasmid harbouring erm(B) could be a source of antibiotic resistance transmission in emerging L. garvieae infection in aquaculture.

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“…In fact, the maintenance of large, non-transmissible plasmids, such as pLG50, despite their high cost to the bacteria, depends on the positive selection of genes with a high adaptive value: virulence genes, bacteriocins, immunity proteins, etc. [54,72].…”

Section: Recombination Between L Garvieae and Other Species Of The Cl...mentioning

confidence: 99%

Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis

et al. 2022

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Lactococcus garvieae is a well-known pathogen of fish, but is rarely involved in infections in humans and other mammals. In humans, the main clinical manifestation of L. garvieae infections is endocarditis usually related to the ingestion of contaminated food, such as undercooked fish and shellfish. This study presents the first complete genomic sequence of a clinical L. garvieae strain isolated from a patient with endocarditis and its comparative analysis with other genomes. This human isolate contains a circular chromosome of 2 099 060 bp and one plasmid of 50 557 bp. In comparison with other fully sequenced L. garvieae strains, the chromosomal DNA of L. garvieae Lg-Granada carries a low proportion of insertion sequence elements and a higher number of putative prophages. Our results show that, in general, L. garvieae is a highly recombinogenic species with an open pangenome in which almost 30 % of its genome has undergone horizontal transfers. Within the genus Lactococcus, L. lactis is the main donor of genetic components to L. garvieae but, taking Lg-Granada as a representative, this bacterium tends to import more genes from Bacilli taxa than from other Lactococcus species.

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Complete Genome Sequence of Nonagglutinating Lactococcus garvieae Strain 122061 Isolated from Yellowtail in Japan

Abstract: Nonagglutinating Lactococcus garvieae has been isolated from diseased farmed yellowtail in Japan since 2012. In this study, the complete genome and plasmid sequence of nonagglutinating L. garvieae strain 122061 was determined, to our knowledge, for the first time.

Cited by 16 publications

References 8 publications

Comparative genome analyses of three serotypes of Lactococcus bacteria isolated from diseased cultured striped jack (Pseudocaranx dentex)

Comparative genome analyses of three serotypes of Lactococcus bacteria isolated from diseased cultured striped jack (Pseudocaranx dentex)

Resistance and genomic characterization of a plasmid pkh2101 harbouring erm(B) isolated from emerging fish pathogen Lactococcus garvieae serotype II in Japan

Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis

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