Epidemiological Study of a Food-Borne Outbreak of Enterotoxigenic
            <i>Escherichia coli</i>
            O25:NM by Pulsed-Field Gel Electrophoresis and Randomly Amplified Polymorphic DNA Analysis

Mitsuda, Toshihiro; Muto, Tetsunori; Yamada, Mikiko; Kobayashi, Nozomu; Toba, Masanori; Aihara, Yukoh; Itô, Akira; Yokota, Shumpei

doi:10.1128/jcm.36.3.652-656.1998

Cited by 41 publications

(17 citation statements)

References 21 publications

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“…The strains isolated from the patients of foodpoisoning incidents in Tokyo and Kanazawa were identical as far as we determined. Furthermore, the strain reported by Mitsuda et al (12) showed the profiles of PFGE similar to those of the strains isolated in Tokyo and Kanazawa. One possibility deduced from these results is that the STIb-producing E. coli 025 strains derived from the same ancestor were spread and reproduced in Japan.…”

Section: Discussionsupporting

confidence: 68%

“…One type of ETEC is E. coli which produces only ST. The outbreak of such E. coli infection in the primary schools in Yokohama in 1996 was recently reported by Mitsuda et al (12). The serotype of the strain was 025:NM, and it produced STIb (STIh).…”

mentioning

confidence: 87%

“…Among E. coli 025 strains collected for this study (Table 1), eight strains were isolated from the patients of food poisoning (6,12,16, and T. Suzuki, personal communications), and most of them were reported to produce ST. All of these eight strains were shown to have only STIb (STIh) gene ( Table 1). Seven of these strains produced STIb in the culture supernatant, but one strain (KB790) released STIb only when it was treated with 0.1 % polymyxin B solution as described in Table 1.…”

Section: Detection Ofst Gene In E Coli 025 Strainsmentioning

confidence: 99%

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Molecular Heterogeneity of Heat‐Stable Toxin‐Producing and Non‐Producing Escherichia coli O25 Strains Isolated in Japan

Kaneko¹,

Fujino²,

Sugawara

et al. 2001

Microbiology and Immunology

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Escherichia coli 025 strains that produce heat-stable toxin (ST) have been recently isolated in Japan, and epidemiological study of this type of enterotoxigenic E. coli is required. In this study the heterogeneity of 16 ST-producing and non-producing strains of E. coli 025 was investigated. All eight ST-producing strains were shown to have STIb gene, and seven of them had similar profiles of plasmids, ladderbanding of LPS in SDS-polyacrylamide gel electrophoresis, and chromosomal DNA digestions in pulsedfield gel electrophoresis (PFGE). In contrast, ST-non-producing strains were more heterogeneous in all parameters examined. PFGE of the digested chromosomal DNA with several restriction enzymes was proved to be an effective procedure to compare the closely related strains of E. coli 025.Key words: Escherichia coli 025, Heat-stable toxin, LPS, Pulsed-field gel electrophoresis Enterotoxigenic Escherichia coli (ETEC) which produces heat-labile toxin (LT) and/or heat-stable toxin (ST) is one of the most common enteropathogens for humans, especially for children in developing countries (4,5,14). ETEC was formerly recognized as a pathogen causing diarrhea among travelers from developed countries where they have better hygienic conditions. However, ETEC strains have been disseminated recently in many countries including developed countries by transport of people and import of foods from endemic areas (3, 18). Many cases of ETEC infections have been reported in Japan (10,13,19), and it is speculated that this pathogen stably persists and is reproduced in the environment in Japan.One type of ETEC is E. coli which produces only ST. The outbreak of such E. coli infection in the primary schools in Yokohama in 1996 was recently reported by Mitsuda et al (12). The serotype of the strain was 025:NM, and it produced STIb (STIh). In this case the food polluted with the pathogen was found to be tuna paste served in the school lunch. There were other outbreaks caused by E. coli 025:H20 in Saitama prefecture in 1997 (11). More recently, we have encoun-*Address correspondence to Dr. Kazuyoshi Kawahara, Department of Bacteriology, The Kitasato Institute, 5-9-1, Shirokane, Minato-ku, Tokyo 108-8642, Japan. Tel/Fax: +81-3-5791-6127. E-mail: kawahara-k@kitasato.or.jp 433 tered an outbreak of ETEC infection in a cafeteria of a research institute in Tokyo. The bacterium isolated in high frequency from the stool specimen of the patients was E. coli 025. The patients suffered from diarrhea with slight fever, which continued for 2 or 3 days (T. Suzuki, personal communications). The strains isolated independently from 10 patients were examined by PCR primer sets for enterotoxin (LT, STIa and STIb) genes, shiga toxin (STX) (STXl and STX2) genes, and other virulence genes (invE and ipaH), and were proved to have STIb gene (Y. Kagami, unpublished data). These reports suggested that E. coli 025 strains which produce STIb have been frequently isolated recently in the Kanto district of Japan. Therefore, investigations of the characteristic...

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Section: Discussionsupporting

confidence: 68%

mentioning

confidence: 87%

Section: Detection Ofst Gene In E Coli 025 Strainsmentioning

confidence: 99%

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Molecular Heterogeneity of Heat‐Stable Toxin‐Producing and Non‐Producing Escherichia coli O25 Strains Isolated in Japan

Kaneko¹,

Fujino²,

Sugawara

et al. 2001

Microbiology and Immunology

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“…Consistent with our findings, strains of CS6 populate 15 of these lineages, indicating widespread genomic heterogeneity. PFGE has also been shown to be useful for outbreak [52,53] and nonoutbreak/surveillance of E. coli [54,55]. In this study we selected PFGE as the method for phylogenetic analysis, in part to create a database of ETEC isolates that would form the basis of the PulseNet Middle East E. coli system.…”

Section: Discussionmentioning

confidence: 99%

Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6

El-Gendy

Mansour

Shaheen

et al. 2013

J Infect Dev Ctries

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“…The contamination of fish with E. coli probably occurs during the insufficient handling of fish (Ayulo, Machado, & Scussel, 1994). An outbreak caused of diarrheal illness was reported in Japan, and it was associated with eating tuna paste, contaminated with enterotoxigenic E. coli (Mitsuda, Muto, Ito, & Yokota, 1998).…”

Section: Introductionmentioning

confidence: 99%

Effect of microwave cooking on foodborne pathogens in fish

Ulusoy

Alakavuk

Mol

et al. 2019

J Food Process Preserv

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Considering the popular use of microwave ovens to prepare foods, inhibitory effect of microwave cooking on foodborne pathogens was studied. Whiting and salmon blocks were inoculated either with E. coli O157:H7, Staphylococcus aureus or Listeria monocytogenes, then cooked in microwave oven to the internal temperatures of 50 and 70°C. Cooking of fish fillets to both internal temperatures resulted in approximately 4 log cfu/cm2 reductions in E. coli O157:H7 population. After cooking to the internal temperatures of 50°C S. aureus counts reduced by 2.78 and 1.06 log cfu/cm2 on whiting and salmon fillets, respectively. L. monocytogenes counts reduced by 1.70 and 0.14 log cfu/cm2 on whiting and salmon fillets, respectively, cooked to 50°C. S. aureus and L. monocytogenes counts reduced below detectable level (2 log cfu/cm2) when cooked to 70°C. The results suggest that internal temperature should be higher than 70°C during microwave cooking to destroy completely these pathogens. Practical applications This research shows that pathogens of Listeria monocytogenes, Staphylococcus aureus and E. coli O157: H7 cannot be completely inhibited by microwaving at 50 and 70°C (internal temperatures) in both fish species. The findings revealed that using a microwave oven to cook fish could be a problem regarding pathogen inactivation in terms of food safety and human health. The study may also help microwave oven producers and caterers to prepare the manufacturer's recommendations, and to determine CCPs in HACCP plans.

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Epidemiological Study of a Food-Borne Outbreak of Enterotoxigenic Escherichia coli O25:NM by Pulsed-Field Gel Electrophoresis and Randomly Amplified Polymorphic DNA Analysis

Cited by 41 publications

References 21 publications

Molecular Heterogeneity of Heat‐Stable Toxin‐Producing and Non‐Producing Escherichia coli O25 Strains Isolated in Japan

Molecular Heterogeneity of Heat‐Stable Toxin‐Producing and Non‐Producing Escherichia coli O25 Strains Isolated in Japan

Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6

Effect of microwave cooking on foodborne pathogens in fish

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