Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6

El-Gendy, Atef; Mansour, Adel; Shaheen, Hind I.; Monteville, Marshall R.; Armstrong, Adam W.; El-Sayed, Nasr; Young, Scott W.; Klena, John D.

doi:10.3855/jidc.2454

Cited by 3 publications

(4 citation statements)

References 58 publications

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“…In STEC, Stx2 was prevalent than Stx1, which agreed with several studies [24,25]. LT gene was not detected in ETEC, yet both LT and ST genes were previously reported in human isolates from cases with diarrhea in Egypt [10].…”

Section: Discussionsupporting

confidence: 90%

“…O126-ETEC and O167-ETEC were the reported serogroups in this study. O126-ETEC and O167-ETEC were associated with cases of human diarrhea in Egypt [10] and worldwide [8]. Moreover, O126-ETEC was isolated from more than 30% of human urine samples in Egypt [28].…”

Section: Discussionmentioning

confidence: 99%

“…ETEC causes diarrhea via production of heat-stable and heat-labile enterotoxins encoded by ST and LT genes, respectively. ETEC isolates are the most common cause of endemic diarrhea in children in developing countries [8,10]. In addition, it is the most frequent etiology of travelers' diarrhea [8].…”

Section: Introductionmentioning

confidence: 99%

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Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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Introduction: Milk is an important food in Egypt and most of it is sold as raw milk in informal markets. Methodology: This study was conducted to investigate the public health risks of Escherichia coli and Staphylococcus aureus in milk sold in informal markets in Egypt. A total of 121 milk samples were analyzed for occurrence, virulence genes and antibiotic resistance of E. coli and S. aureus. Results: A total of 35/121 (28.9%) of milk samples were contaminated with 16/121 (13.2%) E. coli, 22/121 (18.2%) S. aureus, and 3/121 (2.5%) both isolates. Shiga-toxin producing E. coli (STEC), Enterotoxigenic E. coli (ETEC) and Enterotoxigenic S. aureus were detected in 5/121 (4.1%), 2/121 (1.7%) and 8/121 (6.6%) of the examined milk samples, respectively. Multiple drug resistances (MDRs) were showed by 14/16 (87.5%) and 21/22 (95.5%) of E. coli and S. aureus isolates, respectively. E. coli isolates showed high resistance for cephalothin (87.5%), ampicillin (68.8%) and tetracycline (68.8%), but were sensitive for gentamicin and chloramphenicol. Resistance phenotypes of E. coli were diverse; however, STEC isolates were significantly associated with co-resistance to cephalothin, ampicillin and tetracycline (P< 0.05). Two (9.1%) of S. aureus isolates were methicillin-resistant (MRSA) but sensitive to gentamicin (GS-MRSA). Five (22.7%) of S. aureus isolates were gentamicin-resistant methicillin-sensitive S. aureus (GR-MSSA). S. aureus isolates also showed high resistance for ampicillin (100%), tetracycline (90.1%) and sulfamethoxazole-trimethoprim (90.1%). Conclusion: These findings highlighted the potential public health hazards of E. coli and S. aureus pathogens in raw milk sold in informal markets in Egypt.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Elmonir

Abo-Remela

Sobeih

2018

J Infect Dev Ctries

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“…In addition, no resistance to ceftriaxone, ceftazidime, cefepime, ciprofloxacin, and piperacillin-tazobactam was detected among ETEC isolates (Guerra et al, 2014 ). In other study, 47% of ETEC isolates were resistant to ampicillin, a third (37%) of the isolates was resistant to trimethoprim-sulfamethoxazole, and 24% of the isolates were tetracycline-resistant (El-Gendy et al, 2013 ).…”

Section: Discussionmentioning

confidence: 87%

CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence

et al. 2014

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Background: Enterotoxigenic Escherichia coli (ETEC) colonize the human intestinal mucosa using pili and non-pili colonization factors (CFs). CS21 (also designated Longus) is one of the most prevalent CFs encoded by a 14 kb lng DNA cluster located in a virulence plasmid of ETEC; yet limited information is available on the prevalence of CS21 positive ETEC isolates in different countries. The aim of this study was to evaluate the prevalence of CS21 among ETEC clinical isolates from Mexican and Bangladeshi children under 5 years old with diarrhea and to determine the phenotypic and genotypic features of these isolates.Methods: ETEC clinical isolates positive to lngA gene were characterized by genotype, multidrug-resistance, self-aggregation, biofilm formation, and adherence to HT-29 cell line.Results: A collection of 303 E. coli clinical isolates were analyzed, the 81.51% (247/303) were identified as ETEC, 30.76% (76/247) were st+/lt+, and 25.10% (62/247) were positive for the lngA gene. Among the lngA+ ETECs identified, 50% of isolates (31/62) were positive for LngA protein. The most frequent serotype was O128ac:H12 found in 19.35% (12/62) of lngA+ ETEC studied. Multidrug-resistance (MDR) lngA+ ETEC isolates was identified in 65% (39/60), self-aggregation in 48.38% (30/62), and biofilm formation in 83.87% (52/62). ETEC lngA+ isolates were able to adhere to HT-29 cells at different levels. Two lngA isogenic mutants were constructed in the ETEC E9034A and ETEC73332 clinical isolate, showing a 77% and 98% reduction in adherence, respectively with respect to the wild type.Conclusion: ETEC isolates that have the lngA gene showed features associated with self-aggregation, and adherence to HT-29 cells, important characteristics in the human gut colonization process and pathogenesis.

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Microarray-based detection of resistance and virulence factors in commensal Escherichia coli from livestock and farmers in Egypt

Gwida

Awad

El-Ashker

et al. 2020

Veterinary Microbiology

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Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6

Abstract: Our results show a lack of clonality among Egypt CS6 E. coli isolates and supports the use and the further research on vaccines targeting this cell surface antigen.

Cited by 3 publications

References 58 publications

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

Public health risks of Escherichia coli and Staphylococcus aureus in raw bovine milk sold in informal markets in Egypt

CS21 positive multidrug-resistant ETEC clinical isolates from children with diarrhea are associated with self-aggregation, and adherence

Microarray-based detection of resistance and virulence factors in commensal Escherichia coli from livestock and farmers in Egypt

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