Epidemiological Investigation of Outbreak of Brucellosis at Private Dairy Farm, Central Punjab-Pakistan

Kaleem, Muhammad Mohsin; Durrani, Aneela Zameer; Rizwan, Muhammad; Arain, Muhammad Asif; Saeed, Muhammad; Bhutto, Zohaib Ahmed; Kasi, Khushal Khan; Bacha, Umar

doi:10.14737/journal.aavs/2016/4.8.394.397

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…Although our study could not find statistically significant association for breed of the animals with brucellosis, the crossbred and exotic cattle have been previously reported to be more prone to the infection as compared to the local/indigenous breeds [49,[56][57][58][59]. Specifically, within the cattle, breeds, i.e., Sahiwal, Cholistani, and crossbred, univariate analysis did not show statistical significance (p > 0.05) with the infection (Table 3).…”

Section: Discussioncontrasting

confidence: 95%

“…At the farms level, institutional-owned livestock farms tend to be less susceptible to the infection, maybe because of better screening, culling, hygiene and veterinary health facilitation programs than private livestock farms and a statistically significant difference (p < 0.05) has been reported [21,34,39,41], however disagreement does exist [42]. One of the major causes of brucellosis outbreaks especially at private-owned farms is the breach in biosecurity, i.e., the introduction of carrier animals (i.e., most often subclinical infected animals) into the existing herd without prior screening [4,49]. The infection remains unsuspected until abortion storm occurs and/or animals are screened for brucellosis.…”

Section: Discussionmentioning

confidence: 99%

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Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Jamil

Melzer

Saqib

et al. 2020

IJERPH

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Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). The seroprevalence along with 95% confidence interval (CI) was determined. Univariable and multivariable analysis of the epidemiological background data was conducted and odds ratio (OR) was calculated to understand any association between the risk factors and the seroprevalence. An overall seroprevalence of 3.9% (Positive/Tested = 32/828) and 3.3% (27/828) was detected by RBPT and iELISA, respectively. The seroprevalence of 5.6% (CI 3.6–8.3) and 4.7%, (CI 2.8–7.2) and the odds ratio of 2.63 (CI 1.20–5.77) and 2.50 (CI 1.08–5.78) for testing positive by RBPT and iELISA, respectively were significantly higher (p < 0.05) in buffaloes than in cattle. Breed, sex, history of abortion and retention of fetal membranes (RFM) in the animals were not found statistically significantly associated with the infection. RBPT and iELISA based results agreed almost perfect (k = 0.877). In total, Brucella abortus-DNA (9/27) was amplified from seropositive samples by real-time polymerase chain reaction. This study identified for the first time the etiological agents of brucellosis at a molecular level at institutional-owned livestock farms in Pakistan.

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Section: Discussioncontrasting

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Jamil

Melzer

Saqib

et al. 2020

IJERPH

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“…Both of these types of dogs often have access to the rejected flesh from slaughterhouses, butcheries or municipal dumps, dead animal carcasses and remains of livestock, e.g. placentas or aborted fetal material, and also to kitchen leftovers [47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

et al. 2019

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Brucellosis is an important bacterial zoonosis caused by B. abortus and B. melitensis in Pakistan. The status of canine brucellosis caused by B. canis remains obscure. In total, 181 serum samples were collected from stray and working dogs in two different prefectures viz. Faisalabad (n = 87) and Bahawalpur (n = 94). Presence of antibodies against B. canis and B. abortus/B. melitensis was determined using the slow agglutination test (SAT) and ELISA, respectively. Real-time PCR was performed to detect and differentiate Brucella DNA at the species level. In Faisalabad, the serological prevalence was found to be 9.2% (8/87) and 10.3% (9/87) by SAT and ELISA, respectively. Only one of the ELISA positive samples (1.15%) yielded amplification for B. abortus DNA. In Bahawalpur, 63.8% (60/94) samples were found positive by SAT; however, none of the samples was positive by ELISA or by real-time PCR. Location, age (≥1 year) and body condition (weak) were found to be associated with B. canis infection, whereas presence of wounds was found to be associated with B. abortus infection only. These findings point towards a risk of transmission from dog to livestock and humans and vice versa. The study expects to draw the attention of concerned authorities towards infection prevention and animal welfare. This study warrants further epidemiological investigation on brucellosis in pet dogs and their owners. To the best of our knowledge, this is first ever report on B. canis and B. abortus in dogs in Pakistan.

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“…Epidemiologic variables influencing brucellosis prevalence have been studied to determine the risk. Higher disease prevalence was correlated with animal genetics as buffaloes, crossbred and exotic cattle have higher rates of brucellosis (23,28,40,57,63). It has been hypothesized that a specific allele on nramp1 in Sahiwal cows increases brucellosis resistance, but data are limited so far (74).…”

Section: Brucellosis In Animalsmentioning

confidence: 99%

Animal and Human Brucellosis in Pakistan

Jamil

Khan

Saqib

et al. 2021

Front. Public Health

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Brucellosis is a bacterial zoonotic disease that affects many animal species and can be transmitted to humans via direct contact or via contaminated food. Although brucellosis is a serious health hazard, its public health concern has been neglected in many countries. In some developing countries, such as Pakistan, where brucellosis is endemic, this disease continues to be of importance. A literature search for the past 11 years (2011–2021) provided a comprehensive insight into brucellosis in Pakistan. In this review, particular emphasis was placed on occurrence, diagnostic tests used, and prevention, treatment, and control in the context of the “One Health” approach.

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Epidemiological Investigation of Outbreak of Brucellosis at Private Dairy Farm, Central Punjab-Pakistan

Cited by 4 publications

References 10 publications

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Serological and Molecular Investigation of Brucella Species in Dogs in Pakistan

Animal and Human Brucellosis in Pakistan

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