Animal and Human Brucellosis in Pakistan

Jamil, Tariq; Khan, Aman U.; Saqib, Muhammad; Hussain, Muhammad Hammad; Melzer, Falk; Rehman, Abdul; Shabbir, Muhammad Zubair; Khan, Mumtaz Ali; Ali, Shahzad; Khan, Iahtasham; Iqbal, Mudassar; Ullah, Qudrat; Ahmad, Waqas; Mansoor, Muhammad; Neubauer, Heinrich; Schwarz, Štefan

doi:10.3389/fpubh.2021.660508

Cited by 33 publications

(39 citation statements)

References 63 publications

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“…Antimicrobials most frequently used to treat Brucellosis are tetracyclines, aminoglycosides, and fluoroquinolones. 2 , 7 To prevent relapses, combination regimens, Doxycycline with Rifampicin or Gentamycin or Trimethoprim-Sulfamethoxazole and Rifampin are used. Moreover, massive livestock vaccination, hygiene practices, and the pasteurization of milk products ensure prevention.…”

Section: Introductionmentioning

confidence: 99%

Pediatric Brucellosis: A Challenging Diagnosis—Case Report

Shaikh

Sarfaraz

Ehsan

2023

J Prim Care Community Health

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Background: Brucellosis is the second most widely spread zoonotic disease. There is less literature on this disease in Pakistan, leading to delayed diagnosis, or the patient remains undiagnosed. This study aims to contribute to Pediatric brucellosis literature, epidemiological, clinical features, laboratory findings, and treatment. Case Presentation: We present an 11-year-old child who was admitted to the hospital due to abdominal pain for one month and a fever for 15 days. On abdominal ultrasound, she had hepato-splenomegaly with minimal pleural effusion. A comprehensive diagnostic workup for infectious and immunologic disorders confirmed brucellosis with the antibody tests report. She received doxycycline, rifampin, and trimethoprim-sulfamethoxazole for three months. The treatment was continued with Syrup Doxycycline (50 mg/5 ml), and Syrup Rifampicin (2 g/100 ml) was prescribed for five weeks. Her symptoms were improved by the end of the treatment. Conclusion: Brucella is an intracellular pathogen affecting multi-systems of the human body; thus, the treatment is started with antimicrobials that have penetrative effects on a cell. The treatment can be adjusted based on the age group and the complication of the symptoms.

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Section: Introductionmentioning

confidence: 99%

Pediatric Brucellosis: A Challenging Diagnosis—Case Report

Shaikh

Sarfaraz

Ehsan

2023

J Prim Care Community Health

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“…Brucellosis is a zoonotic disease caused by Brucella species. It is mainly transmitted to humans by consuming unpasteurized dairy products or contact with livestock (1,2). Brucellosis is endemic in Latin America, Africa, and the Middle East, including Iran (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Bilateral Prosthetic Knee Joint Infection Caused by Brucella melitensis: A Rare Case Report from Iran

Shokouhi

Tehrani

Saleh

et al. 2022

Jundishapur J Microbiol

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Introduction: Brucella prosthetic joint infection is a rare condition. We report a case of bilateral prosthetic knee joint infection caused by Brucella melitensis, which was cured by prolonged antibiotic therapy without implant removal. Case Presentation: A 62-year-old woman was admitted to the Labbafinejad Hospital (Tehran, Iran), complaining of pain and swelling in her knee joints from two months ago. She was also suffering from intermittent fever and night sweats. She underwent bilateral total knee arthroplasty five years ago because of a severe degenerative joint disease. Agglutination tests (wright and 2-mercaptoethanol (2-ME)) were positive. Her knee joint fluid and blood cultures yielded B. melitensis. The polymerase chain reaction result from her knee joint fluid was positive for Brucella spp. The patient was cured after taking a combination of therapies with doxycycline, rifampin, and gentamicin. The prosthesis was retained due to the lack of loosening in radiography. Ten months after the treatment, the patient had no symptoms and could walk with no pain. Conclusions: Clinicians should consider brucellosis in the differential diagnosis of prosthetic joint infection in the endemic regions. They should also be aware that if patients have no sign of implant loosening, they can achieve favorable outcomes only by using antibiotics and with no need for implant removal.

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“…Each species of Brucella has some host preferences, where the most common species that affect human are B. Melitensis of small ruminants, B. Abortus of large ruminants and B. suis of swine [3]. However, despite the fact that the reservoir hosts may be developed due to an infection in non-preferred hosts [4], the disease has a serious negative impact on farm animals. Concerning sheep, the disease causes great economic losses due to its negative effects on reproductive performance, including abortion, decreased fertility, and reduced milk yield [5].…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

et al. 2022

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Background and Aim: Brucellosis is a zoonotic disease with a worldwide distribution. It has a serious impact on the health of humans and animals, along with a negative impact on the economy. This study aimed to prepare and evaluate the diagnostic performance of a lateral flow immunochromatographic test (LFIT) nanogold diagnostic kit for detecting brucellosis in sheep. Materials and Methods: A rapidly developed LFIT, in which lipopolysaccharide conjugates with nanogold molecules, was placed on the conjugate pad. One hundred ovine serum samples were tested to detect Brucella antibodies (Ab) using the prepared lateral flow immunochromatography assay (LFA) kit and Rose Bengal test. The evaluation of specificity, sensitivity, and accuracy for LFIT and Rose Bengal plate test was conducted using the P04310-10 IDEXX brucellosis ovine/ caprine Ab enzyme-linked immunosorbent assay (ELISA) test (gold standard). Results: The lower amount of Brucella Ab in the ovine serum samples was detected and was 1.58 S/P ratio ELISA titer/100 μL using LFIT and with Rose Bengal to detect 1.86 S/P ratio ELISA. The results showed that the developed LFIT had high specificity with no cross-reactivity with other tested bacteria. The calculated sensitivity, specificity, and accuracy of LFIT and Rose Bengal test using the P04310-10 IDEXX brucellosis ovine/caprine Ab ELISA test (gold standard) were 74% and 89%, 81% and 59%, and 76.9% and 66%, respectively. Conclusion: The present results showed interesting results implying that the LFIA strip test could be used as a substantial diagnostic tool for field screening ovine Brucella as an essential step in the control of brucellosis. However, further studies for the validation of the present findings are necessary.

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Animal and Human Brucellosis in Pakistan

Cited by 33 publications

References 63 publications

Pediatric Brucellosis: A Challenging Diagnosis—Case Report

Pediatric Brucellosis: A Challenging Diagnosis—Case Report

Bilateral Prosthetic Knee Joint Infection Caused by Brucella melitensis: A Rare Case Report from Iran

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

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