Epidemiological aspects of visceral leishmaniasis in Jaciara, Mato Grosso, Brazil, 2003 to 2012

Brito, Veruska Nogueira de; Oliveira, Cenita Maria; Lazari, Patrícia; Sousa, Valéria Régia Franco

doi:10.1590/s1984-29612014008

Cited by 11 publications

(10 citation statements)

References 20 publications

(21 reference statements)

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“…Anti-Leishmania spp. antibodies in the states of Mato Grosso and Mato Grosso do Sul have been reported by other researchers, with frequencies higher than that observed in this work and according to the particular characteristics of each region under study (NUNES et al, 2001;CORTADA et al, 2004;ANDRADE et al, 2009;ALMEIDA et al, 2012;SOUSA et al, 2013;BRITO et al, 2014). This may be related to the opportunist feeding behavior of the vector.…”

Section: Discussioncontrasting

confidence: 47%

“…Positive IFA dogs were positive by ELISA using recombinant peptides of L. infantum chagasi and this is relevant because the ELISA is a specific test with a medium high sensitivity (GRAMICCIA, 2011), and is currently used for confirmation of seroreagent or undetermined immunochromatographic test dogs, which is recommended for screening by the Ministry of Health of Brazil (BRASIL, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Considering the observation of crossreactivity in IFA among different species of Leishmania spp., Trypanosoma cruzi (SOLANO-GALLEGO et al, 2009), and Trypanosoma caninum (ALVES et al, 2012), the presence of seroreagent dogs for more than one antigen suggests the possibility of a co-infection. Thus, although this test is no longer officially used by the Ministry of Health of Brazil (BRASIL, 2016), it is an important tool for the identification of L. infantum chagasi infection in the dogs from Pantanal region, where there are few studies about leishmaniasis…”

Section: Discussionmentioning

confidence: 99%

“…This parasite is of medical and veterinary public health importance because it infects numerous mammal species, including humans, and is transmitted by the bite of female phlebotomine sand flies of the genus Phlebotomus in the old world and Lutzomyia in the new world. The leishmaniases may have different clinical manifestations such as American visceral leishmaniasis (AVL) and American cutaneous leishmaniasis (ACL) (ASHFORD, 2000;CHAPPUIS et al, 2007;HEUSSER JÚNIOR et al, 2010;BRASIL, 2016). In Brazil the cutaneous form of the disease is usually caused by Leishmania braziliensis and Leishmania amazonensis (BRASIL, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The principal vectors are Lutzomyia longipalpis and Lutzomyia cruzi, and the latter is implicated in the transmission of AVL in Corumbá and Ladário, two cities situated in the Pantanal wetland biome in the state of Mato Grosso do Sul (SANTOS et al, 1998;LAINSON;RANGEL, 2005;BRASIL, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Rural area of the Brazilian Pantanal wetlands associated with the occurrence of anti-<i>Leishmania</i> spp. antibodies in dogs

Melo

Costa

Miyazaki

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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An evaluation was made of the presence of anti-Leishmania infantum chagasi antibodies in domestic dogs from the urban and rural areas of Brazil's Pantanal wetland region using serological techniques. A total of 429 dogs were sampled in three areas of the Pantanal biome, including the municipalities of Poconé, Santo Antônio de Leverger, and Barão de Melgaço, in the state of Mato Grosso, and in the municipality of Corumbá, in the state of Mato Grosso do Sul. The immunofluorescence assay (IFA) was used to detect antibodies (cut-off point 40) using Leishmania infantum chagasi antigen. Because of the possibility of cross-reactivity between species of the genus Leishmania, samples that were positive in the IFA against L. infantum chagasi were also tested by IFA in the same conditions, using L. amazonensis and L. braziliensis antigens. IFA-positive samples to L. infantum chagasi were also evaluated using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed the presence of antibodies against L. infantum chagasi in 23 (5.36%; 95% CI: 3.50%-8.05%) dogs and at least one seroreactive dog was found in each of the municipalities evaluated in this study. Antibody titers ranged from 40 to 5,120, and all IFA positive samples were positive in the ELISA. Among the 23 positive dogs, nine were also were seroreactive for L. amazonensis and L. braziliensis antigens. The occurrence of anti-L. infantum chagasi antibodies in dogs was higher in rural areas (7.06%) than in urban areas (2.50%) (P < 0.05). Based on this study, we concluded that dogs from rural areas of the Pantanal wetlands were in contact with Leishmania species, which is relevant information given their importance to public health. Keywords: Dog. Leishmania spp. Antibodies. IFA. ELISA. Pantanal. ResumoNeste trabalho foi realizada uma avaliação sobre a presença de anticorpos anti-Leishmania infantum chagasi em cães domésticos das áreas urbanas e rurais da região do Pantanal brasileiro usando técnicas sorológicas. Um total de 429 cães foram amostrados em três áreas do bioma do Pantanal, incluindo os municípios de Poconé, Santo Antônio de Leverger e Barão de Melgaço, em Mato Grosso, e o município de Corumbá, em Mato Grosso do Sul. A reação de imunofluorescência indireta (RIFI) foi utilizada para detectar anticorpos (ponto de corte de 40) de Leishmania infantum chagasi como antígeno. Devido à possibilidade de reação cruzada entre as espécies do gênero Leishmania, as amostras positivas na RIFI para L. infantum chagasi foram também avaliadas na RIFI utilizando L. amazonensis e L. braziliensis como antígenos. As amostras positivas na RIFI para L. infantum chagasi foram avaliadas utilizando o ensaio de imunoadsorção ligado à enzima (ELISA). Os resultados mostraram a presença de anticorpos contra L. infantum chagasi em 23 (5,36%; IC 95%: 3,50% -8,05%) cães e pelo menos um cão soro-reativo foi encontrado em todos os municípios avaliados neste estudo. Os títulos de anticorpos variaram de 40 a 5.120 e todas as amostras positivas na RIFI foram positivas no ELISA. Entre...

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