Eosinophils in Haemonchus contortus-infected resistant and susceptible breeds of sheep: Abomasal tissue recruitment and in vitro functional state

Terefe, Getachew; Lacroux, Caroline; Prévot, Françoise; Grisez, Christelle; Bergeaud, Jean-Paul; Bleuart, Céline; Dorchies, P.; Foucras, Gilles; Jacquiet, P.

doi:10.1016/j.vetpar.2009.06.041

Cited by 29 publications

(23 citation statements)

References 19 publications

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“…A steady leukocyte count response in cases of gastrointestinal nematode infection, especially regarding abomasal eosinophil counts, was observed by Amarante et al, (2005) and Terefe et al, (2007) when studying sheep that were resistant and susceptible to H. contortus infection. This was seen because mobilization of effector cells is important in the early days of infection (TEREFE et al, 2009). This argument may also be an explanation for our findings, since the sheep had chronic infection.…”

Section: Discussionmentioning

confidence: 99%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

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Section: Discussionmentioning

confidence: 99%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Peripheral blood eosinophilia is associated with GIN parasite infections and has been consistently reported to be higher in sheep resistant to H. contortus , T. circumcincta , and T. colubriformis [70–73]. Such findings led to the evaluation of peripheral blood eosinophilia as a potential marker of GIN parasite resistance.…”

Section: Ideal Characteristics Of Diagnostic Markers For Gin Parasmentioning

confidence: 99%

Current Status for Gastrointestinal Nematode Diagnosis in Small Ruminants: Where Are We and Where Are We Going?

Preston

Sandeman

González

et al. 2014

Journal of Immunology Research

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Gastrointestinal nematode (GIN) parasites pose a significant economic burden particularly in small ruminant production systems. Anthelmintic resistance is a serious concern to the effective control of GIN parasites and has fuelled the focus to design and promote sustainable control of practices of parasite control. Many facets of sustainable GIN parasite control programs rely on the ability to diagnose infection both qualitatively and quantitatively. Diagnostics are required to determine anthelmintic efficacies, for targeted treatment programs and selection of animals for parasite resistant breeding. This review describes much of the research investigated to date to improve the current diagnostic for the above practices which is based on counting the number of parasite eggs in faeces.

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“…Mucus production hinders attachment of the GIN to the gut wall and accelerates expulsion of the parasite (Artis, 2006;Bancroft et al, 2000;Horsnell et al, 2007;McKenzie et al, 1998;Urban et al, 1998;Zhao et al, 2003). Very few studies have examined the immune protective role of IL-13 and its relationship to resistance of small ruminants to Haemonchus contortus, (Jasmer, Lahmers, & Brown, 2007;Lacroux et al, 2006;Robinson, Pleasance, Piedrafita, & Meeusen, 2011;Terefe et al, 2009) and the innate and or adaptive immune mechanism involved. Therefore, this study evaluated IL-13 expression in intestinal tissues of goats pasture exposed to Haemonchus contortus.…”

Section: Introductionmentioning

confidence: 99%

Interleukin 13 as a Biomarker for Parasite Resistance in Goats Naturally Exposed to Haemonchus contortus

Corley¹,

Jarmon²

2012

JAS

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Gut expulsion of some mammalian nematodes requires IL-13 secreted by Th2 cells. Interleukin 13 enhances gut contractions and glycoprotein hyper-secretion that propel parasites to detach from the gut wall. Haemonchus contortus is a gastrointestinal blood sucking nematode of small ruminants. This study evaluated expression of IL-13 in selected parasite resistant Spanish and Myotonic goats. Whole blood, and abomasal and intestinal tissues were harvested from goats exhibiting susceptibility and resistance to Haemonchus contortus through standard and molecular detection methods. An indirect Enzyme Linked Immunosorbent Assay was performed to determine IL-13 expression. Results showed that IL-13 was expressed 70% more in intestinal than abomasal tissues. Parasite resistant goats expressed more IL-13 than susceptible goats. These data indicate that IL-13 expression may be useful as a biomarker for resistance to Haemonchus contortus infection in goats, allowing IL-13 based anthelmintic drug development and goat producers the ability to select parasite resistant animals.

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Eosinophils in Haemonchus contortus-infected resistant and susceptible breeds of sheep: Abomasal tissue recruitment and in vitro functional state

Cited by 29 publications

References 19 publications

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Current Status for Gastrointestinal Nematode Diagnosis in Small Ruminants: Where Are We and Where Are We Going?

Interleukin 13 as a Biomarker for Parasite Resistance in Goats Naturally Exposed to Haemonchus contortus

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