Eosinophils as mediators of antibody-dependent damage to schistosomula

Butterworth, Anthony E.; Sturrock, R. F.; Houba, V.; Mahmoud, Adel A. F.; Sher, Alan; Rees, Philip

doi:10.1038/256727a0

Cited by 333 publications

(110 citation statements)

References 9 publications

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“…In previous studies, it was shown that antibody-dependent cell-mediated damage to schistosomula was ablated when the unpurified human blood leukocytes used as the effector cells were first treated with anti-eosinophil serum and complement, whereas treatment with antineutrophil serum had relatively little effect (13). This, together with the findings that mononuclear cells were not active, strongly suggested that the eosinophil was the cell primarily responsible for mediating damage in this reaction.…”

Section: Discussionmentioning

confidence: 58%

“…It can be seen that all but one of the cell preparations studied contained 83-98.5% eosinophils; the remaining cells were mainly lymphocytes and monocytes which have been shown to be inactive (see next section and Ref. 13). It is of note that four of the populations studied contained no detectable neutrophils, and three contained only 1% neutrophils.…”

Section: Cytotoxic Activity Of Eosinophil-enriched Cell Preparationsmentioning

confidence: 91%

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Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Butterworth¹,

David²,

Franks³

et al. 1977

The Journal of Experimental Medicine

174

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After earlier observations that antibody-dependent, cell-mediated damage to 51Cr-labeled schistosomula can be ablated by pretreatment of a mixed preparation of human peripheral blood leukocytes with an anti-eosinophil serum and complement, we investigated the cytotoxic effects of eosinophil-enriched cell preparations. Preparations containing up to 98.5% eosinophils and devoid of neutrophils were effective in mediating antibody-dependent damage to schistosomula. Preparations enriched in mononuclear cells or in neutrophils, and devoid of eosinophils, were inactive. Eosinophils from some patients with eosinophilia induced by schistosomiasis were less active on a cell-to-cell basis than cells from normal individuals. The possibility that such cells were initially blocked by immune complexes was considered, and it was found that reasonable cytotoxicity by purified eosinophils from patients with eosinophilia could be generated by overnight cultures. A possible requirement for cooperation between eosinophils and other cell types was also studied. Lymphocytes, neutrophils and monocytes failed to enhance eosinophil-mediated cytotoxicity. These results provide further evidence that the eosinophil is the only cell in man responsible for antibody-dependent, complement-independent damage to schistosomula in vitro. Eosinophils from individuals, however, differ in their cytotoxic potential by a mechanism yet to be elucidated. The possible relationship of these findings to immunity in vivo is discussed.

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Section: Discussionmentioning

confidence: 58%

Section: Cytotoxic Activity Of Eosinophil-enriched Cell Preparationsmentioning

confidence: 91%

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Butterworth¹,

David²,

Franks³

et al. 1977

The Journal of Experimental Medicine

174

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“…The anti-CD200R3 Ab has side effects as it engages the activating CD200R3 receptor that is expressed not only on basophils but also on mast cells, leading to systemic mast cell activation (50). Interestingly, eosinophil deficiency also had no impact on granuloma size and disease progression although eosinophils are abundant in granulomas and can kill schistosoma migratory larvae (or schistosomulae) in vitro (43,(51)(52)(53). At present, we cannot exclude the possibility that eosinophils and basophils have redundant functions so that lack of basophils is compensated by the presence of eosinophils and vice versa.…”

Section: Discussionmentioning

confidence: 99%

T Cell–Derived IL-4/IL-13 Protects Mice against Fatal Schistosoma mansoni Infection Independently of Basophils

Schwartz

Oeser

Costa

et al. 2014

The Journal of Immunology

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Schistosomiasis is a severe and chronic disease caused by the parasitic trematode Schistosoma mansoni after deposition of eggs in the liver and intestines. The immune response to S. mansoni eggs is characterized by increased Th2 cells, eosinophilia, and high serum IgE levels. Granulomas are formed around the eggs to protect the organs against tissue damage caused by toxic products that are secreted from the eggs. Egg-derived components have further been shown to activate the IgE-mediated release of IL-4 and IL-13 from basophils, suggesting that basophils could be involved in protection against a fatal course of infection. Using T cell–specific IL-4/IL-13–deficient mice and basophil-deficient Mcpt8Cre mice, we determined the contribution of Th2 cells and basophils for protective immunity against S. mansoni egg–induced pathology during the patent stage of infection. Our results demonstrate that T cell–derived IL-4/IL-13 was essential for granuloma formation, IgE production, basophilia, differentiation of alternatively activated macrophages, and protection against fatal infection. Although basophils were recruited into liver granulomas, they appeared to be dispensable as a source of IL-4/IL-13 both for differentiation of Th2 cells and for prevention of weight loss and mortality.

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“…However, the role played by these cells is not completely elucidated. In vitro studies have shown that eosinophils might be efficient anti-parasite effectors by their ability to kill schistosomula in the presence of immune sera (Butterworth et al 1975, Anwar et al 1979. However, in vivo evidence for such a role is sparse and equivocal , Sher et al 1990).…”

Section: Discussionmentioning

confidence: 99%

Involvement of regional lymph nodes after penetration of Schistosoma mansoni cercariae in naive and infected mice

Lozzi¹,

Machado²,

Gerken³

et al. 1996

Mem. Inst. Oswaldo Cruz

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Eosinophils as mediators of antibody-dependent damage to schistosomula

Cited by 333 publications

References 9 publications

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

Antibody-dependent eosinophil-mediated damage to 51Cr-labeled schistosomula of Schistosoma mansoni: damage by purieid eosinophils.

T Cell–Derived IL-4/IL-13 Protects Mice against Fatal Schistosoma mansoni Infection Independently of Basophils

Involvement of regional lymph nodes after penetration of Schistosoma mansoni cercariae in naive and infected mice

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